LOCUS       62056_20925        4767 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(972..1049)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1279..1307
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1315..1331
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1344..2051
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    complement(2055..2111)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2112..2128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2421..2449
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2497..3684
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      4010..4598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggaagcgga agagccaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
       61 caagcggtcc acgtggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      121 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
      181 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
      241 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
      301 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
      361 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
      421 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
      481 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
      541 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
      601 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
      661 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
      721 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
      781 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
      841 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
      901 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
      961 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1021 cgaaaggttt tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga
     1081 attgcaagct gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1141 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1201 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1261 atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
     1321 gcggataaca atttcacaca ggaatggtga gcaagggcga ggaggataac atggccatca
     1381 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     1441 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1501 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     1561 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1621 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1681 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1741 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1801 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1861 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1921 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1981 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     2041 agctgtacaa gtaaaagctt gcatgcatgc aggtcgactc tagaggatcc ccgggtaccg
     2101 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2161 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2221 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcca cctgacgtct
     2341 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2401 gtcttcaaga attctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     2461 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     2521 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     2581 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     2641 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     2701 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     2761 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     2821 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     2881 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     2941 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     3001 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     3061 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     3121 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     3181 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     3241 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     3301 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     3361 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     3421 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     3481 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     3541 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     3601 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     3661 gcatggagcc gggccacctc gacctgaatg gaaggtctcg cggtatcatt gcagcactgg
     3721 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3781 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4141 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4741 cgaccgagcg cagcgagtca gtgagcg
//
