LOCUS       62056_20920        3379 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      287..675
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    710..726
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        793..821
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     RBS             836..858
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             869..1576
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1586..1632
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2307..3098)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ccataaaccg cccagtctag ctatcgtcat gtaagccccc tgcaagctac ctctttctct
       61 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtagca
      121 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
      181 tgcttgcggc agcgcgtttc cgagaaccgc gcgaacgaca tggagcggca cgcgggcgtg
      241 gaaagcctgg tcggctggat ccggccacga tgcgtccggc gtagaggatc tgaagatcag
      301 cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg
      361 tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac gtactaagct
      421 ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat
      481 aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa
      541 aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg
      601 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
      661 acttaacggc tgacatggga attccacatg tggaattcca catgtggaat tgtgagcgga
      721 taacaatttg tggaattccc gggagagcta ctgactctta tacacaagtt cggccacggc
      781 ctaggcccat ggttgacaat taatcatcgg ctcgtataat gcctctagaa ataattttgt
      841 ttaactttaa gaaggagata tactcgagat ggtgagcaag ggcgaggagg ataacatggc
      901 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      961 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1021 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     1081 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     1141 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     1201 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1261 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1321 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1381 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1441 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1501 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1561 ggacgagctg tacaagtagg agctccaaat aaaacgaaaa gctcagtcga aagactgggc
     1621 ctttcgtttt atgcggccgc agcttgtcga cgaattcccg gggatccggt gattgattga
     1681 gcaagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct
     1741 ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     1801 cggatcaatt cccctgctca cacagactgg gtaccaagct ctcggtaaca tcaaggcccg
     1861 atccttggag cccttgccct cccgacgatg atcgtgccgt gatcgaaatc cagatccttg
     1921 acccacagtt gcaaaccctc actgatccgt cccgtccggg tcgaccaaag cggccatcgt
     1981 gcctccccac tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc tggtttcctg
     2041 gatgccgacg gatttgcact gccggtagaa ctccgcgagg tcgtccagcc tcaggcagca
     2101 gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
     2161 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     2221 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     2281 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     2341 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     2401 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     2461 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2521 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2581 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2641 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2701 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2761 actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat
     2821 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     2881 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     2941 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     3001 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     3061 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     3121 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     3181 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     3241 cttgctgtcc ataaaaccgc ccagtctagc tatcgtcatg taagcccact gcaagctacc
     3301 tctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagaagctg acattcatcc
     3361 cggggtagca ccggctttc
//
