LOCUS       40924_42504        4915 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac-VvSTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4915)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4915)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..1183
                     /note="Stilbene synthase 2 from Vitis vinifera. Accession#:
                     P51070"
     terminator      1435..1521
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1613..1640
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1660..1751
                     /label=AmpR promoter
     primer_bind     complement(2064..2080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2323..3135
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3667..4212
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4849..4877
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4885..4901
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagacatatg gcaagtgtcg aggaattccg caatgctcag cgcgcaaagg gtcccgccac
       61 gatcctggcc attggaacag caacgcccga tcattgcgtg taccaatcgg actacgcgga
      121 tttttacttc cgcgtgacaa agtcagaaca catgactgca cttaaaaaga aattcaaccg
      181 tatttgcgat aagtccatga tcaagaaacg ctacatccac cttacagaag aaatgttaga
      241 ggaacatcct aacattggag cctatatggc accttcatta aacattcgcc aggaaattat
      301 taccgcggag gtccccaaac ttgggaagga ggccgcttta aaagctctta aggaatgggg
      361 tcagcctaag tctaaaatca cgcatttggt cttctgcact acatctgggg tggagatgcc
      421 cggagcagat tacaaattag cgaacttgtt gggacttgaa ccctccgtcc gtcgcgtcat
      481 gctgtaccat cagggctgct atgccggagg gactgtgctg cgcactgcta aagacttagc
      541 agaaaacaat gctggggctc gtgtcctggt tgtttgttcc gagatcaccg ttgtgacttt
      601 ccgcgggcca agtgaggatg ctcttgattc cttagtcggg caagctcttt tcggcgatgg
      661 ctcagcagcg gttattgtcg gctcagatcc agacattagc atcgagcgtc cactgtttca
      721 actggtgtca gcggctcaaa cctttatccc gaacagcgca ggagccattg caggcaactt
      781 acgcgaagtt ggattaacgt tccatctgtg gccgaatgtg cccaccctga ttagcgaaaa
      841 tattgaaaag tgcttaactc aggcatttga tccgttgggc atcagcgact ggaattcctt
      901 gttttggatc gcccatccag gggggcctgc gattttggac gccgtagaag ctaaactgaa
      961 tcttgacaag aaaaaattgg aggcaacacg tcatgtgctg tccgagtatg gcaacatgtc
     1021 cagtgcatgc gtgcttttca tcttggacga gatgcgtaag aaaagtttaa aaggggaacg
     1081 tgctacgacc ggggagggtt tagactgggg agtgttgttt ggttttggtc ccgggctgac
     1141 tatcgaaaca gtggtacttc attccattcc gatggttacc aattaatggt acccggggat
     1201 cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga gagaagattt
     1261 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     1321 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     1381 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     1441 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1501 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     1561 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     1621 atcctgacgg atggcctttt tgcgtttcta caaactcttt ttgtttattt ttctaaatac
     1681 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1741 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1801 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1861 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1921 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1981 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2041 agaatgactt ggttgagtaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2101 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2161 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gggggggggg ggaaagccac
     2221 gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     2281 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     2341 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2401 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     2461 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2521 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2581 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
     2641 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2701 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2761 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2821 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
     2881 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2941 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3001 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3061 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3121 ctcgatgagt ttttctaagc attggtaacc tggtatgagt cagcaacacc ttcttcacga
     3181 ggcagacctc agcgcccccc ccccccgcaa actattaact ggcgaactac ttactctagc
     3241 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3301 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3361 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3421 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3481 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3541 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3601 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacccct taataagatg
     3661 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc
     3721 cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg
     3781 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga
     3841 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat
     3901 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
     3961 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     4021 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     4081 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     4141 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     4201 agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg
     4261 catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga
     4321 ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct
     4381 gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc
     4441 agtgccaaca tagtaagcca gtatacactc cgctagcaag gagatggcgc ccaacagtcc
     4501 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     4561 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     4621 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atccggagct tatcgactgc
     4681 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     4741 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     4801 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
     4861 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaa
//
