LOCUS       V002827                 4810 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002827
VERSION     V002827
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4810)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4810)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..203
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    211..227
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             246..1361
                     /gene="rppA"
                     /label="1,3,6,8-tetrahydroxynaphthalene synthase"
                     /note="1,3,6,8-tetrahydroxynaphthalene synthase from
                     Streptomyces griseus. Accession#: Q54240"
     CDS             complement(1729..2517)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(2518..2609)
                     /label="AmpR promoter"
     rep_origin      complement(2657..3395)
                     /direction=LEFT
                     /label="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(3571..3592)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3608..4687)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4688..4765)
                     /label="lacI promoter"
ORIGIN
        1 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
       61 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
      181 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagaatggc gaccctgtgc cgaccggcca tcgctgtgcc cgagcacgtc atcacgatgc
      301 agcagaccct ggacctggcc cgggagaccc atgccgggca cccgcagcgc gacctcgtcc
      361 tgaggctcat ccagaacacc ggcgtccaga cccggcacct cgtgcagccc atcgagaaga
      421 ccctggcgca ccccggattc gaggtgcgca accaggtgta cgaggccgag gccaagaccc
      481 gggtccccga agtcgtccgg cgggcgctcg ccaacgccga gaccgagccg tccgagatcg
      541 acctgatcgt ctacgtctcc tgcacgggtt tcatgatgcc ctcgctgacc gcgtggatca
      601 tcaacagcat gggcttccgg cccgagaccc gccaactgcc catcgcccag ctcggctgtg
      661 cggcgggcgg cgcggcgatc aaccgcgcgc acgacttctg cgtggcctac cccgactcca
      721 acgtcctcat cgtgtcctgc gagttctgct cgctgtgcta ccagcccacc gacatcgggg
      781 tcggttccct gctctccaac ggactcttcg gcgacgcgct ctccgcggcc gtcgtacggg
      841 gacagggcgg caccggcatg cgcctggagc gcaacggctc ccacctggtg cccgacaccg
      901 aggactggat ctcctacgcg gtccgcgaca ccgggttcca cttccagctg gacaagcggg
      961 tcccgggcac catggagatg ctcgccccgg tgctcctgga cctggtcgac ctgcacggct
     1021 ggtccgtccc gaacatggac ttcttcatcg tccacgcggg cggaccgcgc atcctggacg
     1081 acctctgcca cttcctcgac ctgccgcccg agatgttccg ctacagccgg gccaccctca
     1141 ccgaacgcgg caacatcgcg agctccgtcg tcttcgacgc gctggcgcgc ctcttcgacg
     1201 acggcggcgc cgccgagtcc gcgcaggggc tcatcgccgg cttcggtccc ggcatcaccg
     1261 ccgaggtggc cgtggggagt tgggccaagg aaggcctcgg ggcggacgtc ggacgcgacc
     1321 tcgacgagct ggagctgacc gccggcgttg cgctgtccgg ctaatggtac ccggggatcc
     1381 tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc
     1441 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     1501 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccctgaa
     1561 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     1621 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc
     1681 gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac
     1741 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     1801 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     1861 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     1921 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     1981 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     2041 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     2101 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     2161 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     2221 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     2281 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     2341 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     2401 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     2461 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatact
     2521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2581 atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct acgctccggg
     2641 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     2701 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
     2761 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
     2821 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     2881 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     2941 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
     3001 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     3061 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
     3121 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     3181 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     3241 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     3301 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     3361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
     3421 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3481 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     3541 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     3601 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3661 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     3721 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     3781 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     3841 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     3901 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     3961 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     4021 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     4081 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     4141 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     4201 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     4261 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     4321 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     4381 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     4441 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     4501 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     4561 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     4621 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     4681 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     4741 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     4801 attaggaaat
//
