LOCUS       40924_42464        9068 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac-Pg6MSAS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 9068)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..5330
                     /note="6-methylsalicylic acid synthase from Penicillium
                     patulum. Accession#: P22367"
     terminator      5588..5674
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5766..5793
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        5813..5904
                     /label=AmpR promoter
     primer_bind     complement(6217..6233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6476..7288
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7820..8365
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        9002..9030
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    9038..9054
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagaattcat gcattccgct gcaacttcta catacccctc tgggaaaaca tccccagcac
       61 cagtcggaac ccctgggact gagtacagtg aatatgaatt ctccaacgat gtggcggtag
      121 tgggaatggc ctgccgcgta gcgggaggta accacaaccc tgaacttctc tggcaatcgc
      181 tcctcagcca gaagagtgca atgggtgaga ttccacccat gcggtgggag ccttactatc
      241 gtcgggatgc acgaaatgag aaattcctca agaacacaac gtcgcgtggc tatttcctag
      301 atcgcttgga ggactttgac tgtcagttct ttggcatctc ccctaaggag gccgagcaga
      361 tggaccccca acagcgagtc tctctggaag tggcatcgga agccctggaa gatgccggta
      421 ttcctgccaa gagtctgtct gggagtgaca cagcagtctt ctggggtgtt aactcagacg
      481 actactccaa gctagtgcta gaggatctgc caaatgtcga ggcgtggatg ggcattggca
      541 ctgcatactg tggcgtgccc aatcgcatct catatcacct caacttgatg ggtcctagca
      601 ccgccgtgga tgcggcgtgt gcttcgtctc tcgtcgccat acatcatgga gttcaggcca
      661 tccgactggg agaatcaaag gtggctattg tcggaggtgt caatgccctc tgcggcccag
      721 gactgacccg cgtcctcgac aaagccggcg ccatctcgtc tgacggatct tgcaagtcct
      781 tcgatgatga cgcccatggg tacgctagag gtgaaggtgc cggcgccctc gtcctcaaaa
      841 gtctgcaccg tgcattgctc gaccacgata atgtcctggc agtcattaag ggcagtgcgg
      901 tatgccagga tggcaagacc aacggcatca tggcccccaa ttccgtggcg caacagctag
      961 cagcaaacaa tgctctctct gctgcaaata tcgaccctca caccgtgcgc tatgtggaag
     1021 cacatgccac atccacgccc ttgggcgacc caactgaaat atccgccatt gcaagtgtct
     1081 acggcgccga ccgccccgcg gatgatccat gctacatcgg gtccatcaag cccaatattg
     1141 gtcacctgga ggctggtgcg ggagtcatgg gcttcatcaa agcagttctg gccatccaaa
     1201 agggcgtcct gccaccgcag gccaacttga caaagctcaa tagccggatc gactggaaga
     1261 cagccggtgt taaggtcgtt caagaggcca ccccgtggcc cgaatcagac cccatccgta
     1321 gagcaggtgt ctgctcgtat gggtacggag gtactgtttc tcacgcggtg atcgaagaat
     1381 tcagccctat cctgcagcca gacccactag gtaatggagc agtcagcgga ccaggccttc
     1441 ttttattgtc cggaccccag gagaaacgtt tagccttgca ggccaaaaca ttgcgcgact
     1501 ggatgactgc cgaaggaaag gatcacaatt tgagtgatat tctcactact ctagctactc
     1561 gacgagatca ccacgactac cgagcggcgc ttgtagttga tgactaccgt gatgctgagc
     1621 aagtcctgca atcactggcc aatggggtcg accatacatt cacgacccag agccgtgtac
     1681 tgggctcaga catcagcaag gacgttgtct gggtgttttc tgggcacggt gcgcagtggc
     1741 cggacatggg caagcaactt attcacaacc cggtcttctt cgcagccatc cagccactcg
     1801 atgagttgat ccaagccgag attgggctgt cccctattga gctactccgg acgggtgact
     1861 ttgagtcgtc tgatcgggta cagatcctta cttatgtcat gcaaattggt ctcagcgccc
     1921 tgcttcaaag caatggcatc actccacagg ccgtgattgg acactccgtg ggcgaaatcg
     1981 cggccagtgt tgtggctgga gcactatccc cagcagaggg tgcgcttatt gtcacccgca
     2041 gagcgttgct gtaccgccag gttatgggca agggcggcat gatccttgtc aacctcccta
     2101 gtgccgagac tgaagagatc ttaggttccc gatcagacct ggttgtggcc attgactcgt
     2161 cgccatcttc ctgtgtggtg gctggagata aggagcttgt ggccgaaaca gcagaggctc
     2221 tcaaggcacg aggcgtgaag acattcactg tgaagagtga tattgccttc cacagtccaa
     2281 ccttgaatgg gctggtcgat cctcttcggg atgtgctcgc agagactctg tcccctgtga
     2341 gtcccaacgt caagttgtat tccaccgcac tggcagaccc ccgcggccaa gacctgcgcg
     2401 acgtggaata ctgggccggc aacatggtca accgcgtgcg tctcacttcg gctgtcaagg
     2461 ccgctgttga ggacggatat cgtctcttcc ttgaagtctc aacccaccca gtggtgtctc
     2521 actctatcaa cgagacacta atggatgcag gcatggaaga ctttgcggtc atcccaactc
     2581 tccttcggaa aaagccaact gagaagcata tcctacacag cattgcacag cttcactgcc
     2641 gcggtgcgga agtgaactgg gcggcacaga tgcccggacg ctgggccact ggagttccca
     2701 ccacgacctg gatgcataaa cccatctggc gcaagataga gactgcgccg cttcacactg
     2761 gtctcacgca tgatgtcgag aagcacacac ttctgggaca gcgtatccca gtgcccggca
     2821 ccgacaccta tgtctacaca acccggctgg ataacgacac aaagcccttc ccgggcagcc
     2881 accctctgca cggcaccgag atcgttccgg ctgcaggttt gatcaatact ttcttgaagg
     2941 gcacaggcgg ccagatgctg cagaacgttg ttctccgcgt tcccgttgct attaacgcac
     3001 cacgatccgt ccaggtcgta gtgcagcagg atcaagtcaa ggttgtgtct cgactgatcc
     3061 cgagtgagcc ctcgcagttg gatgacgatg cctcctgggt cactcacacc actgcatact
     3121 gggatcggaa ggttgctggt tccgaagatc gcatcgactt cgctgccgtc aaatcgcgac
     3181 tggtaaccaa actagcagac aacttttcga ttgactacct ggacaaggtg ggtgtatctg
     3241 ccatgggatt cccatgggcg gtaacagagc actaccgcaa tgacaaggag atgctggcac
     3301 gtgttgatgt caatcctgcc atctcgggag atgcccccct gccctgggat tcttcatcct
     3361 gggccccagt tctcgacgct gccacctccg tcggttcaac catcttcccg acacctgcac
     3421 ttcggatgcc cgcccagatt gagcgggtag aggtcttcac ctcgcaggat ccgcccaaga
     3481 tcagctggct ctacgtccag gaggcctccg attcggtgcc cacctctcac gttagcgtgg
     3541 tgagcgaggc gggtgaggtc cttgccaaat tcacagccat gcggttctcc gagattgaag
     3601 gcactcctgg cgtcagtggc agcatggaga gtctcgtgca ccagatcgct tggccaccag
     3661 ccaccccggc cgaagagccc ttgtccatcg agacagtgat cctggtctcg cccgatgcta
     3721 ccaccagggc cctgtatgcc gcaagtctgc cgacccgggt taactccttc cagttctcct
     3781 ctacacagga gttcttcagc aacgcttcat cccttcccct ggagaaggga accgtggtga
     3841 cctacatccc cggtgaggtc gcctcgctgg ccgaggttcc cgccgcatcc gagtcattca
     3901 cttggaactt gctcgaacta attaaattca ccgtcaatgg ctcactgcct atcaaagtct
     3961 tcactcttac cgcgaacatc ggcgagggtc aaacacctac cgctctagcc cagtccccat
     4021 tgtacggcct ggcacgggtc atcgccagtg agcaccctga tctaggaacc ctcatcgacg
     4081 ttgaagagcc cgtcatcccc ctgtcgacga tgcgatacat ccagggggca gacatcatcc
     4141 gaatcaacga cggaatcgct cgcacctccc ggttccgcag cctgccgcgc aacaagctgc
     4201 tccctgccag cgaaggtcct cgcctgcttc cccgccccga gggcacatac ctgatcaccg
     4261 gtggtcttgg cgtcctcggt ctcgaggtcg ctgacttcct ggtcgagaag ggtgccagac
     4321 gtctgctgct catctcgcga cgcgcccttc cccctcgccg aacctgggac caggttagcg
     4381 aggatctcca gccgaccatc gccaagatcc gcctcctgga gagtcgcggc gcctcggtcc
     4441 atgtcctgcc cctggacatc accaaaccgg atgcggtcga gcagcttacc acggcgttgg
     4501 accgactttc cctaccttcg gtccagggtg tcgttcacgc tgccggtgtg ctggacaacg
     4561 agctagtgat gcagaccact cgcgacgcct tcaaccgggt tctcgcaccc aagattgcgg
     4621 gcgcgctagc cctgcatgaa gtcttccccc ctaagagcgt cgacttcttc gtcatgttct
     4681 cctcttgtgg aaacctcgtc gggttcactg gtcaagcttc ctacggcagt ggtaacgcct
     4741 tcttggatac actagccacc caccgtgcgc gcctaggtga cgcagccgtg tccttccagt
     4801 ggacatcgtg gcgtggcctt ggcatgggtg ccagcacaga ctttatcaac gctgaattgg
     4861 aatccaaagg aatcacggac gtgacgcgcg atgaggcctt tgctgcatgg cagcatctcg
     4921 ccaagtatga tatggaccac ggagttgttt tgcgaagccg tgcctttgag gacggggaac
     4981 cgattccagt gtctattctc aatgatatcg cggtgcgacg cgtgggcact gtgtctaaca
     5041 cctcgccggc ggctgccggt tccagcgacg cggtgcctac gtctggaccg gagcttaagg
     5101 cttatttgga cgagaagatc aggggctgtg tggccaaggt gttacagatg actgctgagg
     5161 atgtggattc aaaggcagct ctggccgatc tgggtgttga ctcagtcatg acagtcactc
     5221 tgcgtcgcca gctgcagcta acactgaaga ttgcggtgcc ccctacattg acgtggagtc
     5281 atccgactgt cagccatctg gcggtgtggt ttgctgagaa gcttgccaaa taatgagctc
     5341 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggctg ttttggcgga
     5401 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     5461 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     5521 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     5581 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     5641 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     5701 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     5761 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc tttttgttta
     5821 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5881 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5941 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6001 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6061 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6121 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     6181 atacactatt ctcagaatga cttggttgag taattcactg gccgtcgttt tacaacgtcg
     6241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcggggggg
     6361 gggggaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     6421 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     6481 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
     6541 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     6601 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     6661 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     6721 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     6781 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     6841 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     6901 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     6961 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     7021 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     7081 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     7141 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     7201 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     7261 gtttcatttg atgctcgatg agtttttcta agcattggta acctggtatg agtcagcaac
     7321 accttcttca cgaggcagac ctcagcgccc cccccccccg caaactatta actggcgaac
     7381 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7441 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7501 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7561 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7621 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7681 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7741 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7801 ccttaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa
     7861 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga
     7921 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt
     7981 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt
     8041 ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     8101 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     8161 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt
     8221 aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta
     8281 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     8341 gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta
     8401 agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc
     8461 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat
     8521 cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac
     8581 tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc aaggagatgg
     8641 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     8701 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     8761 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatccgga
     8821 gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
     8881 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
     8941 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
     9001 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
     9061 acaggaaa
//
