LOCUS       V002832                 4828 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002832
VERSION     V002832
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..203
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    211..227
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             249..266
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             267..1379
                     /gene="rppA"
                     /label="1,3,6,8-tetrahydroxynaphthalene synthase"
                     /note="1,3,6,8-tetrahydroxynaphthalene synthase from
                     Streptomyces griseus. Accession#: Q54240"
     regulatory      1426..1573
                     /label="rrnBT1T2"
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     CDS             complement(1747..2535)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(2536..2627)
                     /label="AmpR promoter"
     rep_origin      complement(2675..3413)
                     /direction=LEFT
                     /label="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(3589..3610)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3626..4705)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4706..4783)
                     /label="lacI promoter"
ORIGIN
        1 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
       61 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
      181 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagaatgca ccatcaccat caccatgcga ccctgtgccg accggccatc gctgtgcccg
      301 agcacgtcat cacgatgcag cagaccctgg acctggcccg ggagacccat gccgggcacc
      361 cgcagcgcga cctcgtcctg aggctcatcc agaacaccgg cgtccagacc cggcacctcg
      421 tgcagcccat cgagaagacc ctggcgcacc ccggattcga ggtgcgcaac caggtgtacg
      481 aggccgaggc caagacccgg gtccccgaag tcgtccggcg ggcgctcgcc aacgccgaga
      541 ccgagccgtc cgagatcgac ctgatcgtct acgtctcctg cacgggtttc atgatgccct
      601 cgctgaccgc gtggatcatc aacagcatgg gcttccggcc cgagacccgc caactgccca
      661 tcgcccagct cggctgtgcg gcgggcggcg cggcgatcaa ccgcgcgcac gacttctgcg
      721 tggcctaccc cgactccaac gtcctcatcg tgtcctgcga gttctgctcg ctgtgctacc
      781 agcccaccga catcggggtc ggttccctgc tctccaacgg actcttcggc gacgcgctct
      841 ccgcggccgt cgtacgggga cagggcggca ccggcatgcg cctggagcgc aacggctccc
      901 acctggtgcc cgacaccgag gactggatct cctacgcggt ccgcgacacc gggttccact
      961 tccagctgga caagcgggtc ccgggcacca tggagatgct cgccccggtg ctcctggacc
     1021 tggtcgacct gcacggctgg tccgtcccga acatggactt cttcatcgtc cacgcgggcg
     1081 gaccgcgcat cctggacgac ctctgccact tcctcgacct gccgcccgag atgttccgct
     1141 acagccgggc caccctcacc gaacgcggca acatcgcgag ctccgtcgtc ttcgacgcgc
     1201 tggcgcgcct cttcgacgac ggcggcgccg ccgagtccgc gcaggggctc atcgccggct
     1261 tcggtcccgg catcaccgcc gaggtggccg tggggagttg ggccaaggaa ggcctcgggg
     1321 cggacgtcgg acgcgacctc gacgagctgg agctgaccgc cggcgttgcg ctgtccggct
     1381 aatggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc
     1441 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     1501 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     1561 gaagtgaaac gccctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt
     1621 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
     1681 accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag
     1741 acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa
     1801 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc
     1861 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     1921 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     1981 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     2041 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     2101 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     2161 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     2221 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     2281 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     2341 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     2401 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     2461 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     2521 caccgcttcc ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2581 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact
     2641 cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc
     2701 cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg
     2761 gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc
     2821 ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga
     2881 caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc
     2941 gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt
     3001 tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct
     3061 gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg
     3121 agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt
     3181 tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg
     3241 ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag
     3301 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
     3361 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3421 ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     3481 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct
     3541 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
     3601 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     3721 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     3781 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     3841 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     3901 agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     3961 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     4021 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     4081 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     4141 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     4201 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     4261 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     4321 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     4381 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     4441 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     4501 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     4561 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     4621 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     4681 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
     4741 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
     4801 cccttatgcg actcctgcat taggaaat
//
