LOCUS       62056_20890        4776 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(972..1049)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1279..1307
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1315..1331
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1344..2060
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(2064..2120)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2121..2137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2430..2458
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2506..3693
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      4019..4607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggaagcgga agagccaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
       61 caagcggtcc acgtggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      121 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
      181 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
      241 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
      301 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
      361 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
      421 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
      481 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
      541 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
      601 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
      661 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
      721 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
      781 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
      841 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
      901 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
      961 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1021 cgaaaggttt tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga
     1081 attgcaagct gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1141 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1201 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1261 atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
     1321 gcggataaca atttcacaca ggaatggtga gcaagggcga ggagctgttc accggggtgg
     1381 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1441 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1501 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg
     1561 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1621 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1681 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1741 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1801 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1861 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1921 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     1981 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2041 gcatggacga gctgtacaag taaaagcttg catgcctgca ggtcgactct agaggatccc
     2101 cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2161 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2221 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2281 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgccac
     2341 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2401 ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc
     2461 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat
     2521 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
     2581 ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat
     2641 ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca
     2701 ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc
     2761 gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg
     2821 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2881 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     2941 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     3001 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     3061 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     3121 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     3181 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     3241 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     3301 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     3361 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     3421 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     3481 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     3541 ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     3601 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     3661 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aaggtctcgc ggtatcattg
     3721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4741 gcagccgaac gaccgagcgc agcgagtcag tgagcg
//
