LOCUS       40924_42434        6685 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac-At4CL1m-opr-VvSTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 6685)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6685)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..1690
                     /gene="4CL1"
                     /label=4CL1
                     /note="4-coumarate--CoA ligase 1 from Arabidopsis thaliana.
                     Accession#: Q42524"
     CDS             1757..2932
                     /note="Stilbene synthase 2 from Vitis vinifera. Accession#:
                     P51070"
     terminator      3205..3291
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3383..3410
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3430..3521
                     /label=AmpR promoter
     primer_bind     complement(3834..3850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4093..4905
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5437..5982
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      6544..6572
                     /label=Tac promoter
                     /note="Tac promoter"
                     /regulatory_class="promoter"
     promoter        6619..6647
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6655..6671
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagacatatg gcgccacaag aacaagcagt ttctcaggtg atggagaaac agagcaacaa
       61 caacaacagt gacgtcattt tccgatcaaa gttaccggat atttacatcc cgaaccacct
      121 atctctccac gactacatct tccaaaacat ctccgaattc gccactaagc cttgcctaat
      181 caacggacca accggccacg tgtacactta ctccgacgtc cacgtcatct cccgccaaat
      241 cgccgccaat tttcacaaac tcggcgttaa ccaaaacgac gtcgtcatgc tcctcctccc
      301 aaactgtccc gaattcgtcc tctctttcct cgccgcctcc ttccgcggcg caaccgccac
      361 cgccgcaaac cctttcttca ctccggcgga gatagctaaa caagccaaag cctccaacac
      421 caaactcata atcaccgaag ctcgttacgt cgacaaaatc aaaccacttc aaaacgacga
      481 cggagtagtc atcgtctgca tcgacgacaa cgaatccgtg ccaatccctg aaggctgcct
      541 ccgcttcacc gagttgactc agtcgacaac cgaggcatca gaagtcatcg actcggtgga
      601 gatttcaccg gacgacgtgg tggcactacc ttactcctct ggcacgacgg gattaccaaa
      661 aggagtgatg ctgactcaca agggactagt cacgagcgtt gctcagcaag tcgacggcga
      721 gaacccgaat ctttatttcc acagcgatga cgtcctactc tgtgttttgc ccatgtttca
      781 tatctacgct ttgaactcga tcatgttgtg tggtcttaga gttggtgcgg cgattctgat
      841 aatgccgaag tttgagatca atctgctatt ggagctgatc cagaggtgta aagtgacggt
      901 ggctccgatg gttccgccga ttgtgttggc cattgcgaag tcttcggaga cggagaagta
      961 tgatttgagc tcgataagag tggtgaaatc tggtgctgct cctcttggta aagaacttga
     1021 agatgccgtt aatgccaagt ttcctaatgc caaactcggt cagggatacg gaatgacgga
     1081 agcaggtcca gtgctagcaa tgtcgttagg ttttgcaaag gaaccttttc cggttaagtc
     1141 aggagcttgt ggtactgttg taagaaatgc tgagatgaaa atagttgatc cagacaccgg
     1201 agattctctt tcgaggaaac aacccggtga gatttgtatt cgtggtcacc agatcatgaa
     1261 aggttacctc aacaatccgg cagctacagc agagaccatt gataaagacg gttggcttca
     1321 tactggagat attggattga tcgatgacga tgacgagctt ttcatcgttg atcgattgaa
     1381 agaacttgtc aagtataaag gttttcaggt agctccggct gagctagagg ctttgctcat
     1441 cggtcatcct gacattactg atgttgctgt tgtcgcaatg aaagaagaag cagctggtga
     1501 agttcctgtt gcatttgtgg tgaaatcgaa ggattcggag ttatcagaag atgatgtgaa
     1561 gcaattcgtg tcgaaacagg ttgtgtttta caagagaatc aacaaagtgt tcttcactga
     1621 atccattcct aaagctccat cagggaagat attgaggaaa gatctgaggg caaaactagc
     1681 aaatggattg taatggtacc cggggatcct ctagagtcga cctgcaggca tgcaatttca
     1741 cacaggaaac agacatatgg caagtgtcga ggaattccgc aatgctcagc gcgcaaaggg
     1801 tcccgccacg atcctggcca ttggaacagc aacgcccgat cattgcgtgt accaatcgga
     1861 ctacgcggat ttttacttcc gcgtgacaaa gtcagaacac atgactgcac ttaaaaagaa
     1921 attcaaccgt atttgcgata agtccatgat caagaaacgc tacatccacc ttacagaaga
     1981 aatgttagag gaacatccta acattggagc ctatatggca ccttcattaa acattcgcca
     2041 ggaaattatt accgcggagg tccccaaact tgggaaggag gccgctttaa aagctcttaa
     2101 ggaatggggt cagcctaagt ctaaaatcac gcatttggtc ttctgcacta catctggggt
     2161 ggagatgccc ggagcagatt acaaattagc gaacttgttg ggacttgaac cctccgtccg
     2221 tcgcgtcatg ctgtaccatc agggctgcta tgccggaggg actgtgctgc gcactgctaa
     2281 agacttagca gaaaacaatg ctggggctcg tgtcctggtt gtttgttccg agatcaccgt
     2341 tgtgactttc cgcgggccaa gtgaggatgc tcttgattcc ttagtcgggc aagctctttt
     2401 cggcgatggc tcagcagcgg ttattgtcgg ctcagatcca gacattagca tcgagcgtcc
     2461 actgtttcaa ctggtgtcag cggctcaaac ctttatcccg aacagcgcag gagccattgc
     2521 aggcaactta cgcgaagttg gattaacgtt ccatctgtgg ccgaatgtgc ccaccctgat
     2581 tagcgaaaat attgaaaagt gcttaactca ggcatttgat ccgttgggca tcagcgactg
     2641 gaattccttg ttttggatcg cccatccagg ggggcctgcg attttggacg ccgtagaagc
     2701 taaactgaat cttgacaaga aaaaattgga ggcaacacgt catgtgctgt ccgagtatgg
     2761 caacatgtcc agtgcatgcg tgcttttcat cttggacgag atgcgtaaga aaagtttaaa
     2821 aggggaacgt gctacgaccg gggagggttt agactgggga gtgttgtttg gttttggtcc
     2881 cgggctgact atcgaaacag tggtacttca ttccattccg atggttacca attaatggta
     2941 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt gcatgcaagc
     3001 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
     3061 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     3121 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     3181 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3241 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3301 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3361 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3421 caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3601 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3661 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3721 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtaa ttcactggcc
     3841 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3901 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3961 caacagttgc gggggggggg ggaaagccac gttgtgtctc aaaatctctg atgttacatt
     4021 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     4081 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat
     4141 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     4201 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     4261 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     4321 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     4381 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     4441 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     4501 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     4561 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     4621 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     4681 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     4741 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     4801 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     4861 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaagc attggtaacc
     4921 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgcccccc ccccccgcaa
     4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5401 ccactgagcg tcagacccct taataagatg atcttcttga gatcgttttg gtctgcgcgt
     5461 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     5521 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
     5581 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
     5641 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
     5701 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     5761 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     5821 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
     5881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     5941 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
     6001 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
     6061 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
     6121 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
     6181 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
     6241 cgctagcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     6301 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     6361 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     6421 ggcgtagagg atccggagct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca
     6481 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     6541 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     6601 atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
     6661 gcggataaca atttcacaca ggaaa
//
