LOCUS       40924_42419        4820 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTac-5'UTR-Sgr_rppA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   2  (bases 1 to 4820)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (12-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..203
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    211..227
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           231..255
                     /gene="rppA"
     CDS             256..1371
                     /gene="rppA"
                     /label=rppA
                     /note="1,3,6,8-tetrahydroxynaphthalene synthase from 
                     Streptomyces griseus. Accession#: Q54240"
     regulatory      1418..1565
                     /label=rrnBT1T2
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     CDS             complement(1739..2527)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(2528..2619)
                     /label=AmpR promoter
     rep_origin      complement(2667..3405)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(3581..3602)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3618..4697)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4698..4775)
                     /label=lacI promoter
ORIGIN
        1 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
       61 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
      181 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt caataacaag
      241 aaaggagcat cacaaatggc gaccctgtgc cgaccggcca tcgctgtgcc cgagcacgtc
      301 atcacgatgc agcagaccct ggacctggcc cgggagaccc atgccgggca cccgcagcgc
      361 gacctcgtcc tgaggctcat ccagaacacc ggcgtccaga cccggcacct cgtgcagccc
      421 atcgagaaga ccctggcgca ccccggattc gaggtgcgca accaggtgta cgaggccgag
      481 gccaagaccc gggtccccga agtcgtccgg cgggcgctcg ccaacgccga gaccgagccg
      541 tccgagatcg acctgatcgt ctacgtctcc tgcacgggtt tcatgatgcc ctcgctgacc
      601 gcgtggatca tcaacagcat gggcttccgg cccgagaccc gccaactgcc catcgcccag
      661 ctcggctgtg cggcgggcgg cgcggcgatc aaccgcgcgc acgacttctg cgtggcctac
      721 cccgactcca acgtcctcat cgtgtcctgc gagttctgct cgctgtgcta ccagcccacc
      781 gacatcgggg tcggttccct gctctccaac ggactcttcg gcgacgcgct ctccgcggcc
      841 gtcgtacggg gacagggcgg caccggcatg cgcctggagc gcaacggctc ccacctggtg
      901 cccgacaccg aggactggat ctcctacgcg gtccgcgaca ccgggttcca cttccagctg
      961 gacaagcggg tcccgggcac catggagatg ctcgccccgg tgctcctgga cctggtcgac
     1021 ctgcacggct ggtccgtccc gaacatggac ttcttcatcg tccacgcggg cggaccgcgc
     1081 atcctggacg acctctgcca cttcctcgac ctgccgcccg agatgttccg ctacagccgg
     1141 gccaccctca ccgaacgcgg caacatcgcg agctccgtcg tcttcgacgc gctggcgcgc
     1201 ctcttcgacg acggcggcgc cgccgagtcc gcgcaggggc tcatcgccgg cttcggtccc
     1261 ggcatcaccg ccgaggtggc cgtggggagt tgggccaagg aaggcctcgg ggcggacgtc
     1321 ggacgcgacc tcgacgagct ggagctgacc gccggcgttg cgctgtccgg ctaatggtac
     1381 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga
     1441 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     1501 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     1561 acgccctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     1621 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     1681 ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt
     1741 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     1801 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     1861 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     1921 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     1981 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     2041 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     2101 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     2161 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     2221 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     2281 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     2341 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     2401 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     2461 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     2521 ccctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2581 gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct
     2641 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
     2701 ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt
     2761 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc
     2821 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
     2881 aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
     2941 cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag
     3001 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
     3061 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
     3121 ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     3181 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     3241 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     3301 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     3361 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     3421 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     3481 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     3541 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     3601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     3721 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     3781 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     3841 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc
     3901 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     3961 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     4021 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     4081 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     4141 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     4201 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     4261 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     4321 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     4381 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     4441 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     4501 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     4561 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     4621 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     4681 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     4741 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     4801 cgactcctgc attaggaaat
//
