LOCUS       V017011                 5437 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017011
VERSION     V017011
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..951)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(999..1221)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(3090..3113)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(3459..3743)
                     /gene="CI"
                     /label="Repressor protein CI"
                     /note="Repressor protein CI from Enterobacteria phage 434.
                     Accession#: P16117"
     terminator      complement(3945..3972)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4064..4150)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4369..5226)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5227..5331)
                     /label="AmpR promoter"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgactc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg taattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattaaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgcttttcc agtctgacca cttcggctta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggg ctcatcaaca
     1681 ggcttacccg tcgtacggtc aagaggacat ccgggcaaat gcaacgaaag gctcagtcga
     1741 aagactgggc ctttcgttgc gctgcggaga cttagggacc ctaaaacaag atacattgta
     1801 tgaaaataca agaaagtttg ttcattaggc accccgggct ttactcgtaa agcttccggc
     1861 gcgtatgttg tgtcgaccga cctgcaggtg cagtaaaaaa ggaaaaaaat gaaaaaatta
     1921 ttattcgcaa ttcctttatg tctcagctac gaaaccgaaa tcttgaccgt cgaatatggt
     1981 ctgctgccaa tcggcaagat tgttgaaaaa cgtattgaat gtacggtcta ctcagtggat
     2041 aacaacggca atatctacac ccagccggtg gcccagtggc atgaccgtgg tgaacaggaa
     2101 gtgttcgaat attgtctgga agacggatct ttaatccgtg ccacaaagga tcacaaattt
     2161 atgactgtag atggtcagat gctcccaatc gacgaaattt ttgaacgcga attagacctg
     2221 atgcgcgtgg ataatctccc gaattaaacg gagccaatgt acgcaaaaaa ccccgcttca
     2281 gcggggtttt ttcgccaaaa aaaaccccgc cctgtcaggg gcggggtttt tttttagggc
     2341 cctactgact gttagctgga gaccgtgaca agagtgccct gcccccagta gtcaaaaagt
     2401 ccggtgacac agtagtataa cgccgtatca tccgagcgaa ctttgctcat ttgcaaatag
     2461 acagtgtttt cgcaatcgtc tttgctgatg ataaagcgat ccttcaaggc actgttataa
     2521 tcagtggttc cgtcgcccca gatgacgcca atccactcca agccacgccc cggtgcctgg
     2581 cgtacccaat tgaccccata atccgtcaac gagaaaccac tcacagcgca gctcagcttt
     2641 aacgagcctc caggctgcac aagaccacca ccactttcaa gaagcttaac ctcactccct
     2701 ccacccgaag accctccgcc gcctgaaccc ccacctccgg agccaccacc cccgcgttta
     2761 agttcaacct tcgtgccctg accgaagacc caatggttgg agtaccacaa tgcacaatag
     2821 tatgtagcga aatcctctgg ttgcagactg ctgatagtaa gtgtggcctt gtcaccaatc
     2881 aaactccccg aaaaacggct agggacgccc ggtgcgcggt tattagtacc gccaatcaaa
     2941 cccttgaaca acttacccgg tttttcctgg acccacgaag cgtaattact ggttgtgact
     3001 gccccagtgc tcgaacggca tgtaattgta acgcgatctc ccactgacgc tgataagctt
     3061 gatggcgact gcgtcataac aatgtcgcgc ttgtcatcgt cgtccttgta gtctacgaat
     3121 tttaccctcg cttccacgac aacaccgata atcttgcagt ttccgttgat aggagtcata
     3181 ggccatgaag gattcaggcc tttcaggtac ttctgaccgc catctatgac cagtttcttg
     3241 aatgttgctt cgttcgcgtc agtcagtttg gctacaacaa ggcttccatt cactggctcc
     3301 cgtccagtat ctactaacac catatgacct tcagggatgc tttgacctac aggtgaggtc
     3361 atggaatcac cttcaacctt cagccagaat ccattgccta ataagttaac gtcactgtca
     3421 taccattcat caatgtcctt gatatcgtag ggttcacaag cttcacacca cgaacgagct
     3481 ctaaccatgc taatcaatgg atatttccct ttgggctcaa cgtgcccaac aaatctaaca
     3541 ttcgaatcag aggtgccatt gagcagccag tcaacactta cgccaagagc tgacgcaagt
     3601 tctggtaaaa agcgtggtcg cttagtttta ccgttttcga gctgctctat agactgctgg
     3661 gtagtcccca ccttttgagc aagttcagcc tggttaagtc caagctgaat tcttttgctt
     3721 tttaccctgc tgctgatact catttttttt tcctccttac tctagtaaaa gttaaacaaa
     3781 attatttgta gagggaaacc gttgtggtct ccctgaatat agataccgag ccttatgcat
     3841 gcccgtaaag ttatccagca accactcata gacctagggc agcagatagg gacgacgtgg
     3901 tgttagctgt gctctagaat cctcagcgag tttgtagaaa cgcaaaaagg acatccgtca
     3961 ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4021 ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4081 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     4141 gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg
     4201 gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg
     4261 ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg
     4321 ctgaaaatct tctctcatcc gccaaaacag ccatgcccat ggcgtttacc aatgcttaat
     4381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccagcgtc
     4981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5401 taggcgtatc acgaggccct taggccaaat aggccgt
//
