LOCUS       40924_44374        8903 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTSaraSZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial 
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 8903)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     regulatory      complement(976..1025)
                     /label=araC promoter
                     /note="araC promoter"
                     /regulatory_class="promoter"
     terminator      complement(1218..1245)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(1298..1426)
                     /label=similar to SynZIP17
                     /note="similar to SynZIP17"
     misc_feature    complement(1427..1450)
                     /label=similar to linker
                     /note="similar to linker"
     misc_feature    complement(1451..1987)
                     /label=similar to T7RNAP
                     /note="similar to T7RNAP"
     regulatory      complement(1988..2022)
                     /label=N-terminal 500 RU, from Shis et al. 2013
                     /note="N-terminal 500 RU, from Shis et al. 2013"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2029..2159)
                     /label=pBAD
                     /note="pBAD"
                     /regulatory_class="promoter"
     regulatory      2457..2587
                     /label=pBAD
                     /note="pBAD"
                     /regulatory_class="promoter"
     regulatory      2594..2624
                     /label=C-terminal 500 RU, from Shis et al. 2013
                     /note="C-terminal 500 RU, from Shis et al. 2013"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    2625..2750
                     /label=similar to SynZIP18
                     /note="similar to SynZIP18"
     misc_feature    2751..2768
                     /label=similar to linker
                     /note="similar to linker"
     misc_feature    2769..4883
                     /label=similar to T7RNAP
                     /note="similar to T7RNAP"
     terminator      5116..5202
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5294..5321
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        5340..5431
                     /label=AmpR promoter
     rep_origin      5885..6340
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6898..7554)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7555..7657)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(8183..8728)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aatttcaaca cgagccaaaa taataaaaaa gccggattaa taatctggct ttttatattc
     1201 tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagtgt gatccaagcc
     1261 agctcgatat ccgtcggctt gaacgaattg ttagacatta tttgtaagct tcgatttctt
     1321 tacgcaggtt cgcgattttc tgcttcagtt gttcacgttt ctgtttcagc tgttcgatac
     1381 ggttgcgcag ttccgctttt ttggatttca gttcttcttt ttcgttggat ccaccacctg
     1441 aacctccgtt cttgtagacg tgccctacgc gcttgttgag ttgttcctca acgtttttct
     1501 tgaagtgctt agcttcaagg tcacggatac gaccgaagcg agcctcgtcc tcaatggccc
     1561 gaccgattgc gcttgctaca gcctgaacgg ttgtattgtc agcactggtt aggcaagcca
     1621 gagtggtctt aatggtgatg tacgctacgg cttccggctt gatttcttgc aggaactgga
     1681 aggctgtcgg gcgcttgccg cgcttagctt tcacttcctc aaaccagtcg ttgatgcgtg
     1741 caatcatctt agggagtagg gtagtgatga gaggcttggc ggcagcgtta tccgcaacct
     1801 caccagcttt aagttgacgc tcaaacatct tgcggaagcg tgcttcaccc atctcgtaag
     1861 actcatgctc aagggccaac tgttcgcgag ctaaacgctc accgtaatgg tcagccagag
     1921 tgttgaacgg gatagcagcc agttcgatgt cagagaagtc gttcttagcg atgttaatcg
     1981 tgttcatatt agaaaaagaa tgttattaag taagtctttc tagagctcgc tagcccaaaa
     2041 aaacgggtat ggagaaacag tagagagttg cgataaaaag cgtcaggtag gatccgctaa
     2101 tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtc
     2161 ctaggacttt tctgccgtga ttatagacac ttttgtcgcg atgttgtgtc taattttgaa
     2221 gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
     2281 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
     2341 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt
     2401 cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca ctcgagacat
     2461 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
     2521 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg
     2581 ggctagcact agtggagccc tatatataaa gatcagggcc aggtatgagc atcgcggcga
     2641 ccctggagaa cgatctggcg cgtctggaaa acgaaaacgc tcgtctcgaa aaagacatcg
     2701 cgaacctgga acgtgacctg gcgaaactgg agcgtgaaga agcgtacttc ggaggttcag
     2761 gtggtaagaa agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg
     2821 gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca
     2881 tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag
     2941 taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc
     3001 gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc
     3061 cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg
     3121 tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt
     3181 acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg
     3241 tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc
     3301 gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt
     3361 ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca
     3421 gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc
     3481 cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta
     3541 acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt
     3601 actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg
     3661 agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac
     3721 tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg
     3781 agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg
     3841 acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc
     3901 gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga
     3961 aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg
     4021 tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg
     4081 ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc
     4141 tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc
     4201 cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca
     4261 tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga
     4321 actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag
     4381 agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc
     4441 aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct
     4501 tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg
     4561 gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt
     4621 gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca
     4681 ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg
     4741 agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc
     4801 aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag
     4861 agtcggactt cgcgttcgcg taatctagag tcgacctgca ggcatgcaag cttggctgtt
     4921 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     4981 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     5041 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     5101 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     5161 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     5221 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     5281 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     5341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5521 agtaaaagat gctgaagatc agttggggca aactattaac tggcgaacta cttactctag
     5581 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5641 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5701 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5761 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5821 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5881 atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5941 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6001 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6061 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     6121 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6181 agtggactct tgttccaaac ttgaacaaca ctcaacccta tctcgggcta ttcttttgat
     6241 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6301 tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat
     6361 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6421 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6481 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6541 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6601 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6661 cgctctgcta atcctgttac cagtcaggca tttgagaagc acacggtcac actgcttccg
     6721 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     6781 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     6841 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     6901 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     6961 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     7021 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     7081 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     7141 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     7201 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     7261 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     7321 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     7381 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     7441 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     7501 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     7561 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     7621 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     7681 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     7741 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     7801 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     7861 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
     7921 agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca
     7981 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     8041 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     8101 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     8161 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     8221 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8281 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     8341 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     8401 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     8461 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     8521 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     8581 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     8641 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     8701 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
     8761 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     8821 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     8881 ctgctcatgt ttgacagctt atc
//
