LOCUS       62056_21590        5330 bp DNA     circular SYN 09-MAY-2021
DEFINITION  Cloning vector pTS7, complete sequence.
ACCESSION   MW835297
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Schnabel T.
  TITLE     Engineering post-translational regulation of glutamine synthetase 
            for controllable ammonia production in the plant-symbiont A. 
            brasilense
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Schnabel T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2021) Bioengineering, Stanford University, 443 Via
            Ortega, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2021) Bioengineering, Stanford University, 443 Via Ortega, 
            Stanford, CA 94305, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..632)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(725..1870)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(1871..2160)
                     /label=pOri
                     /note="pOri"
                     /regulatory_class="promoter"
     regulatory      2243..2355
                     /label=pKan
                     /note="pKan"
                     /regulatory_class="promoter"
     oriT            complement(2243..2294)
                     /direction=LEFT
                     /note="oriT"
     CDS             2362..3153
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    3180..3240
                     /label=multiple cloning site
                     /note="multiple cloning site"
     oriT            complement(3332..3441)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        3613..3717
                     /label=AmpR promoter
     CDS             3718..4575
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4591..5179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
       61 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
      121 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
      181 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
      241 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
      301 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
      361 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
      421 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
      481 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
      541 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
      601 cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt gccttgctcg
      661 tcggtgatgt acactagtcg ctggctgctg aacccccagc cggaactgac cccacaaggc
      721 cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc
      781 ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat
      841 ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc
      901 tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga
      961 atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc
     1021 aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt
     1081 ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc
     1141 attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct
     1201 cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct
     1261 gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca
     1321 cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt
     1381 tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg
     1441 gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt
     1501 tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt
     1561 ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg
     1621 ccgcctcctg ttcgagacga cgtgaacgct ccacggcggc cgatggcgcg ggcagggcag
     1681 ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc
     1741 cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg
     1801 catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg
     1861 tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt
     1921 attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga
     1981 agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct
     2041 gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa
     2101 aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca
     2161 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     2221 ttgatcggcg cgccccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact
     2281 caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga
     2341 caccaaaagg gagggtacgt aatgattgaa caagatggat tgcacgcagg ttctccggcc
     2401 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2461 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2521 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     2581 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2641 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2701 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2761 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2821 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2881 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     2941 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3001 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3061 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3121 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatgt
     3181 taattaagat tacgccaagc ttgcatgcct gcaggtcgag gatccccggg taccgagctc
     3241 gaattcatgg catagatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     3301 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     3361 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     3421 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     3481 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     3541 ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttcgagcagg tggcactttt
     3601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4561 ctgattaagc attggtaact ggctagccac ttgagatcct ttttttctgc gcgtaatctg
     4621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt gtttaaactc tccctcgcag
     5221 agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacaga gtagacaggc
     5281 tgtctcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt
//
