LOCUS       Exported               19369 bp DNA     circular SYN 29-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        375..720
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             758..1309
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      1347..1547
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    1843..1864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1879..1909
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1917..1933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1941..1957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2505..2850
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9704..9956
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9965..9981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10599..10623
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12219..13367)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13666..14460)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14652..15419
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16042..16753
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(16880..17251)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            17284..17393
                     /note="incP origin of transfer"
     CDS             complement(18074..18724)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    19189..19213
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
       61 gtcgcctaaa gctagcttgc atgcctgcag gtcactattc cagtatggac gattcaaggc
      121 ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt agttcctact
      181 gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact cgccgtgaag
      241 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
      301 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      361 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      421 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      481 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc cgacagtggt
      541 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      601 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
      661 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
      721 cgctgaaatc accagtctct ctctacaaat cggatccatg agcccagaac gacgcccggc
      781 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta
      841 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga
      901 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt
      961 cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact ggacggccga
     1021 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac
     1081 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct
     1141 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct
     1201 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt
     1261 cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgag atcacgcgga
     1321 ctctagagga tcccccgatg agctaagcta gctatatcat caatttatgt attacacata
     1381 atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca gttattgaaa
     1441 tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact ataatacctg
     1501 acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatggg aattcatcga
     1561 tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg gtgattttgc
     1621 tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt taatgaataa
     1681 tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt ttgtctttgg
     1741 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1801 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     1861 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     1921 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcatggg
     1981 acgtcgacct gaggtaatta taacccgggc cctatatatg gatccaattg caatgatcat
     2041 catgacagat ctgcgcgcga tcgatatcag cgctttaaat ttgcgcatgc ctgcaggtca
     2101 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     2161 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     2221 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     2281 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     2341 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     2401 cttcaaagca agtggattga tgtgatggtc aacatggtgg agcacgacac tctcgtctac
     2461 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
     2521 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
     2581 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
     2641 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
     2701 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     2761 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     2821 taaggaagtt catttcattt ggagaggata aaacatttca atcctttgaa cgcggtagaa
     2881 cgtgctaatt ggattttggt gagaacgcgg tagaacgtac ttatcaccta cagttttatt
     2941 ttgtttttct ttttggttta atctatccag cttagtaccg agtgggggaa agtgactggt
     3001 gtgcctaaaa ccttttcttt gatactttgt aaaaatacat acagatacaa tggcgaacgg
     3061 taacttcaag ttgtctcaat tgctcaatgt ggacgagatg tctgctgagc agaggagtca
     3121 tttctttgac ttgatgctga ctaaacctga ttgtgagatc gggcaaatga tgcaaagagt
     3181 tgttgttgat aaagtcgatg acatgattag agaaagaaag actaaagatc cagtgattgt
     3241 tcatgaagtt ctttctcaga aggaacagaa caagttgatg gaaatttatc ctgaattcaa
     3301 tatcgtgttt aaagacgaca aaaacatggt tcatgggttt gcggctgctg agcgaaaact
     3361 acaagcttta ttgcttttag atagagttcc tgctctgcaa gaggtggatg acatcggtgg
     3421 tcaatggtcg ttttgggtaa ctagaggtga gaaaaggatt cattcctgtt gtccaaatct
     3481 agatattcgg gatgatcaga gagaaatttc tcgacagata tttcttactg ctattggtga
     3541 tcaagctaga agtggtaaga gacagatgtc ggagaatgag ctgtggatgt atgaccaatt
     3601 tcgtgaaaat attgctgcgc ctaacgcggt taggtgcaat aatacatatc agggttgtac
     3661 atgtaggggt ttttctgatg gtaagaagaa aggcgcgcag tatgcgatag ctcttcacag
     3721 cctgtatgac ttcaagttga aagacttgat ggctactatg gttgagaaga aaactaaagt
     3781 ggttcatgct gctatgcttt ttgctcctga aagtatgtta gtggacgaag gtccattacc
     3841 ttctgttgac ggttactaca tgaagaagaa cgggaagatc tatttcggtt ttgagaaaga
     3901 tccttccttt tcttacattc atgactggga agagtacaag aagtatctac tggggaagcc
     3961 agtgagttac caagggaatg tgttctactt cgaaccgtgg caggtgagag gagacacaat
     4021 gcttttttcg atctacagga tagctggagt tccgaggagg tctctatcat cgcaagagta
     4081 ctaccgaaga atatatatca gtagatggga aaacatggtt gttgtcccaa ttttcgatct
     4141 ggtcgaatca acgcgagagt tggtcaagaa agacctgttt gtagagaaac aattcatgga
     4201 caagtgtttg gattacatag ctaggttatc tgaccagcag ctgaccataa gcaatgttaa
     4261 atcatacttg agttcaaata attgggtctt attcataaac ggggcggccg tgaagaacaa
     4321 gcaaagtgta gattctcgag atttacagtt gttggctcaa actttgctag tgaaggaaca
     4381 agtggcgaga cctgtcatga gggagttgcg tgaagcaatt ctgactgaga cgaaacctat
     4441 cacgtcattg actgatgtgc tgggtttaat atcaagaaaa ctgtggaagc agtttgctaa
     4501 caagatcgca gtcggcggat tcgttggcat ggttggtact ctaattggat tctatccaaa
     4561 gaaggtacta acctgggcga aggacacacc aaatggtcca gaactatgtt acgagaactc
     4621 gcacaaaacc aaggtgatag tatttctgag tgttgtgtat gccattggag gaatcacgct
     4681 tatgcgtcga gacatccgag atggactggt gaaaaaacta tgtgatatgt ttgatatcaa
     4741 acggggggcc catgtcttag acgttgagaa tccgtgccgc tattatgaaa tcaacgattt
     4801 ctttagcagt ctgtattcgg catctgagtc cggtgagacc gttttaccag atttatccga
     4861 ggtaaaagcc aagtctgata agctattgca gcagaagaaa gaaatcgctg acgagtttct
     4921 aagtgcaaaa ttctctaact attctggcag ttcggtgaga acttctccac catcggtggt
     4981 cggttcatct cgaagcggac tgggtctgtt gttggaagac agtaacgtgc tgacccaagc
     5041 tagagttgga gtttcaagaa aggtagacga tgaggagatc atggagcagt ttctgagtgg
     5101 tcttattgac actgaagcag aaattgacga ggttgtttca gccttttcag ctgaatgtga
     5161 aagaggggaa acaagcggta caaaggtgtt gtgtaaacct ttaacgccac caggatttga
     5221 gaacgtgttg ccagctgtca aacctttggt cagcaaagga aaaacggtca aacgtgtcga
     5281 ttacttccaa gtgatgggag gtgagagatt accaaaaagg ccggttgtca gtggagacga
     5341 ttctgtggac gctagaagag agtttctgta ctacttagat gcggagagag tcgctcaaaa
     5401 tgatgaaatt atgtctctgt atcgtgacta ttcgagagga gttattcgaa ctggaggtca
     5461 gaattacccg cacggactgg gagtgtggga tgtggagatg aagaactggt gcatacgtcc
     5521 agtggtcact gaacatgctt atgtgttcca accagacaaa cgtatggatg attggtcggg
     5581 atacttagaa gtggctgttt gggaacgagg tatgttggtc aacgacttcg cggtcgaaag
     5641 gatgagtgat tatgtcatag tttgcgatca gacgtatctt tgcaataaca ggttgatctt
     5701 ggacaattta agtgccctgg atctaggacc agttaactgt tcttttgaat tagttgacgg
     5761 tgtacctggt tgtggtaagt cgacaatgat tgtcaactca gctaatcctt gtgtcgatgt
     5821 ggttctctct actgggagag cagcaaccga cgacttgatc gagagattcg cgagcaaagg
     5881 ttttccatgc aaattgaaaa ggagagtgaa gacggttgat tcttttttga tgcattgtgt
     5941 tgatggttct ttaaccggag acgtgttgca tttcgatgaa gctctcatgg cccatgctgg
     6001 tatggtgtac ttttgcgctc agatagctgg tgctaaacga tgtatctgtc aaggagatca
     6061 gaatcaaatt tctttcaagc ctagggtatc tcaagttgat ttgaggtttt ctagtctggt
     6121 cggaaagttt gacattgtta cagaaaaaag agaaacttac agaagtccag cagatgtggc
     6181 tgccgtattg aacaagtact atactggaga tgtcagaaca cataacgcga ctgctaattc
     6241 gatgacggtg aggaagattg tgtctaaaga acaggtttct ttgaagcctg gtgctcagta
     6301 cataactttc cttcagtctg agaagaagga gttggtaaat ttgttggcat tgaggaaagt
     6361 ggcagctaaa gtgagtacag tacacgagtc gcaaggagag acattcaaag atgtagtcct
     6421 agtcaggacg aaacctacgg atgactcaat cgctagaggt cgggagtact taatcgtggc
     6481 gttgtcgcgt cacacacaat cacttgtgta tgaaactgtg aaagaggacg atgtaagcaa
     6541 agagatcagg gaaagtgccg cgcttacgaa ggcggctttg gcaagatttt ttgttactga
     6601 gaccgtctta tgacggtttc ggtctaggtt tgatgtcttt agacatcatg aagggccttg
     6661 cgccgttcca gattcaggta cgattacgga cttggagatg tggtacgacg ctttgtttcc
     6721 gggaaattcg ttaagagact caagcctaga cgggtatttg gtggcaacga ctgattgcaa
     6781 tttgcgatta gacaatgtta cgatcaaaag tggaaactgg aaagacaagt ttgctgaaaa
     6841 agaaacgttt ctgaaaccgg ttattcgtac tgctatgcct gacaaaagga agactactca
     6901 gttggagagt ttgttagcat tgcagaaaag gaaccaagcg gcacccgatc tacaagaaaa
     6961 tgtgcacgcg acagttctaa tcgaagagac gatgaagaag ctgaaatctg ttgtctacga
     7021 tgtgggaaaa attcgggctg atcctattgt caatagagct caaatggaga gatggtggag
     7081 aaatcaaagc acagcggtac aggctaaggt agtagcagat gtgagagagt tacatgaaat
     7141 agactattcg tcttacatgt atatgatcaa atctgacgtg aaacctaaga ctgatttaac
     7201 accgcaattt gaatactcag ctctacagac tgttgtgtat cacgagaagt tgatcaactc
     7261 gttgttcggt ccaattttca aagaaattaa tgaacgcaag ttggatgcta tgcaaccaca
     7321 ttttgtgttc aacacgagaa tgacatcgag tgatttaaac gatcgagtga agttcttaaa
     7381 tacggaagcg gcttacgact ttgttgagat agacatgtct aaattcgaca agtcggcaaa
     7441 tcgcttccat ttacaactgc agctggagat ttacaggtta tttgggctag atgagtgggc
     7501 ggccttcctt tgggaggtgt cgcacactca aactactgtg agagatattc aaaatggtat
     7561 gatggcgcat atttggtacc aacaaaagag tggagatgct gatacttata atgcaaattc
     7621 agatagaaca ctgtgtgcac tcttgtctga attaccattg gagaaagcag tcatggttac
     7681 atatggagga gatgactcac tgattgcgtt tcctagagga acgcagtttg ttgatccgtg
     7741 tccaaagttg gctactaagt ggaatttcga gtgcaagatt tttaagtacg atgtcccaat
     7801 gttttgtggg aagttcttgc ttaagacgtc atcgtgttac gagttcgtgc cagatccggt
     7861 aaaagttctg acgaagttgg ggaaaaagag tataaaggat gtgcaacatt tagccgagat
     7921 ctacatctcg ctgaatgatt ccaatagagc tcttgggaac tacatggtgg tatccaaact
     7981 gtccgagtct gtttcagacc ggtatttgta caaaggtgat tctgttcatg cgctttgtgc
     8041 gctatggaag catattaaga gttttacagc tctgtgtaca ttattccgag acgaaaacga
     8101 taaggaattg aacccggcta aggttgattg gaagaaggca cagagagctg tgtcaaactt
     8161 ttacgactgg taatatggaa gacaagtcat tggtcacctt gaagaagaag actttcgaag
     8221 tctcaaaatt ctcaaatcta ggggccattg aattgtttgt ggacggtagg aggaagagac
     8281 cgaagtattt tcacagaaga agagaaactg tcctaaatca tgttggtggg aagaagagtg
     8341 aacacaagtt agacgttttt gaccaaaggg attacaaaat gattaaatct tacgcgtttc
     8401 taaagatagt aggtgtacaa ctagttgtaa catcacatct acctgcagat acgcctgggt
     8461 tcattcaaat cgatctgttg gattcgagac ttactgagaa aagaaagaga ggaaagacta
     8521 ttcagagatt caaagctcga gcttgcgata actgttcagt tgcgcagtac aaggttgaat
     8581 acagtatttc cacacaggag aacgtacttg atgtctggaa ggtgggttgt atttctgagg
     8641 gcgttccggt ctgtgacggt acataccctt tcagtatcga agtgtcgcta atatgggttg
     8701 ctactgattc gactaggcgc ctcaatgtgg aagaactgaa cagttcggat tacattgaag
     8761 gcgattttac cgatcaagag gttttcggtg agttcatgtc tttgaaacaa gtggagatga
     8821 agacgattga ggcgaagtac gatggtcctt acagaccagc tactactaga cctaagtcat
     8881 tattgtcagg tgaagatgtt aagagagcgc ctaataagaa aaactcgtct taatgcataa
     8941 agaaatttat tgtcaatatg acgtgtgtac tcaagggttg tgtgaatgaa gtcactgttc
     9001 ttggtcacga gacgtgtagt atcggtcatg ctaacaaatt gcgaaagcaa gttgctgaca
     9061 tggttggtgt cacacgtagg tgtgcggaaa ataattgtgg atggtttgtc tgtgttgtta
     9121 tcaatgattt tacttttgat gtgtataatt gttgtggccg tagtcacctt gaaaagtgtc
     9181 gtaaacgtgt tgaaacaaga aatcgagaaa tttggaaaca aattcgacga aatcaagctg
     9241 aaaacatgtc tgcgacagct aaaaagtctc ataattcgaa gacctctaag aagaaattca
     9301 aagaggacag agaatttggg acaccaaaaa gatttttaag agatgatgtt cctttcggga
     9361 ttgatcgttt gtttgctttt tgattttatt ttatattgtt atctgtttct gtgtatagac
     9421 tgtttgagat tggcgcttgg ccgactcatt gtcttaccat aggggaacgg actttgtttg
     9481 tgttgttatt ttatttgtat tttattaaaa ttctcaatga tctgaaaagg cctcgaggct
     9541 aagagattat tggggggtga gtaagtactt ttaaagtgat gatggttaca aaggcaaaag
     9601 gggtaaaacc cctcgcctac gtaagcgtta ttacgcccgt ctgtacttat atcagtacac
     9661 tgacgagtcc ctaaaggacg aaacgggccc ctcgaatttc cccgatcgtt caaacatttg
     9721 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     9781 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     9841 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     9901 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     9961 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    10021 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    10081 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
    10141 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10201 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
    10261 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10321 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
    10381 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
    10441 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    10501 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
    10561 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    10621 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    10681 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    10741 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    10801 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    10861 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
    10921 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
    10981 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
    11041 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
    11101 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
    11161 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    11221 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    11281 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    11341 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    11401 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    11461 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    11521 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    11581 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    11641 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    11701 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    11761 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    11821 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    11881 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    11941 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    12001 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    12061 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    12121 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    12181 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    12241 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    12301 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    12361 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    12421 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    12481 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    12541 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    12601 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    12661 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    12721 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    12781 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    12841 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    12901 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    12961 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    13021 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    13081 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    13141 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    13201 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    13261 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    13321 tcgatcagtt cttgcctgta tgtcttccgg tcaaacgtcc gattcattca ccctccttgc
    13381 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    13441 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    13501 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    13561 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    13621 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    13681 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    13741 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    13801 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    13861 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    13921 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    13981 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    14041 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    14101 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    14161 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    14221 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    14281 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    14341 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    14401 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    14461 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    14521 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    14581 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    14641 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    14701 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    14761 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    14821 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    14881 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    14941 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    15001 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    15061 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    15121 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    15181 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    15241 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    15301 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    15361 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    15421 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    15481 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    15541 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    15601 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    15661 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    15721 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    15781 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    15841 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    15901 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    15961 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    16021 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    16081 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    16141 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    16201 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    16261 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    16321 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    16381 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    16441 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    16501 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    16561 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    16621 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    16681 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    16741 gtcggaaagg cgctcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa
    16801 gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc
    16861 gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct
    16921 tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac
    16981 cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc
    17041 ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga
    17101 ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg
    17161 ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc
    17221 ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg
    17281 tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
    17341 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
    17401 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
    17461 cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
    17521 cggaaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    17581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    17641 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    17701 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    17761 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    17821 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    17881 agcgaggaag cggaagagcg ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct
    17941 tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg
    18001 tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg ttgcgctccg
    18061 gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc aacgttcctg acaacgagcc
    18121 tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct gcgtccggac
    18181 cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa
    18241 cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc acggccccaa
    18301 cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa aaacccgcct
    18361 cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc
    18421 cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat
    18481 tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc gtatgattct
    18541 ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt
    18601 aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta
    18661 cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc tgcaactttg
    18721 tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat cagtgataaa
    18781 gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg tgaaacccaa
    18841 catacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca tcggcctgat
    18901 tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg tcaccgccca
    18961 ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt gcctgcgcac ctgtgctggg
    19021 cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc gtctcgctgg ccggcgccag
    19081 atctggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    19141 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    19201 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatcatg
    19261 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
    19321 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcg
//
