LOCUS       Exported                8513 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Exported.
ACCESSION   V000751
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8513)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8513)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1326..2060
                     /gene="ermBP"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             3647..5134
                     /codon_start=1
                     /label=repS
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=97.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     promoter        complement(5847..5949)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6475..7020)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        7132..7160
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(7506..8219)
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     promoter        complement(8227..8511)
                     /label=ldhL promoter
                     /note="L. acidophilus lactate dehydrogenase gene (ldhL) 
                     promoter"
ORIGIN
        1 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
       61 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
      121 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
      181 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
      241 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
      301 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
      361 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
      421 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
      481 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
      541 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
      601 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
      661 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
      721 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcggtc
      781 gagtctagaa tcgatacgat tttgaagtgg caacagataa aaaaaagcag tttaaaattg
      841 ttgctgaact tttaaaacaa gcaaatacaa tcattgtcgc aacagatagc gacagagaag
      901 gcgaaaacat tgcctggtcg atcattcata aagcaaatgc cttttctaaa gataaaacgt
      961 ataaaagact atggatcaat agtttagaaa aagatgtgat ccgtagcggt tttcaaaatt
     1021 tgcaaccagg aatgaattac tatccctttt atcaagaagc gcacaaaaag aaaaacgaaa
     1081 tgatacacca atcagtgcaa aaaaagatat aatgggagat aagacggttc gtgttcgtgc
     1141 tgacttgcac catatcataa aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt
     1201 tatggaaata agacttagaa gcaaacttaa gagtgtgttg atagtgcagt atcttaaaat
     1261 tttgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggagtg
     1321 attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca
     1381 accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag
     1441 gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat
     1501 tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt
     1561 taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg
     1621 ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccatg
     1681 cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc
     1741 gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag
     1801 cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata
     1861 ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca
     1921 atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca
     1981 aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc
     2041 tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg aaagttacac
     2101 gttactaaag ggaatgtaga taaattatta ggtatactac tgacagcttc caaggagcta
     2161 aagaggtccc tagcgctctt atcatgggga agctcggatc atatgcaaga caaaataaac
     2221 tcgcaacagc acttggagaa atgggacgaa tcgagaaaac cctctttacg ctggattaca
     2281 tatctaataa agccgtaagg agacgggttc aaaaaggttt aaataaagga gaagcaatca
     2341 atgcattagc tagaattata ttttttggac aacgtggaga atttagagaa cgtgctctcc
     2401 aagaccagtt acaaagagct agtgcactaa acataattat taacgctata agtgtgtgga
     2461 acactgtata tatggaaaaa gccgtagaag aattaaaagc aagaggagaa tttagagaag
     2521 atttaatgcc atatgcgtgg ccgttaggat gggaacatat caattttctt ggagaataca
     2581 aatttgaagg attacatgac actgggcaaa tgaatttacg tcctttacgt ataaaagagc
     2641 cgttttattc ttaatataac ggctcttttt atagaaaaaa tccttagcgt ggtttttttc
     2701 cgaaatgctg gcggtacccc aagaattaga aatgagtaga tcaaattatt cacgaataga
     2761 atcaggaaaa tcagatccaa ccataaaaac actagaacaa attgcaaagt taactaactc
     2821 aacgctagta gtggatttaa tcccaaatga gccaacagaa ccagaaccag aaacagaatc
     2881 agaacaagta acattggatt tagaaatgga agaagaaaaa agcaatgact tcgtgtgaat
     2941 aatgcacgaa atcgttgctt attttttttt aaaagcggta tactagatat aacgaaacaa
     3001 cgaactgaat agaaacgaaa aaagagccat gacacattta taaaatgttt gacgacattt
     3061 tataaatgca tagcccgata agattgccaa accaacgctt atcagttagt cagatgaact
     3121 cttccctcgt aagaagttat ttaattaact ttgtttgaag acggtatata accgtactat
     3181 cattatatag ggaaatcaga gagttttcaa gtatctaagc tactgaattt aagaattgtt
     3241 aagcaatcaa tcggaaatcg tttgattgct ttttttgtat tcatttatag aaggtggagt
     3301 ttgtatgaat catgatgaat gtaaaactta tataaaaaat agtttattgg agataagaaa
     3361 attagcaaat atctatacac tagaaacgtt taagaaagag ttagaaaaga gaaatatcta
     3421 cttagaaaca aaatcagata agtatttttc ttcggagggg gaagattata tatataagtt
     3481 aatagaaaat aacaaaataa tttattcgat tagtggaaaa aaattgactt ataaaggaaa
     3541 aaaatctttt tcaaaacatg caatattgaa acagttgaat gaaaaagcaa accaagttaa
     3601 ttaaacaacc tattttatag gatttatagg aaaggagaac agctgaatga atatcccttt
     3661 tgttgtagaa actgtgcttc atgacggctt gttaaagtac aaatttaaaa atagtaaaat
     3721 tcgctcaatc actaccaagc caggtaaaag caaaggggct atttttgcgt atcgctcaaa
     3781 atcaagcatg attggcggtc gtggtgttgt tctgacttcc gaggaagcga ttcaagaaaa
     3841 tcaagataca tttacacatt ggacacccaa cgtttatcgt tatggaacgt atgcagacga
     3901 aaaccgttca tacacgaaag gacattctga aaacaattta agacaaatca ataccttctt
     3961 tattgatttt gatattcaca cggcaaaaga aactatttca gcaagcgata ttttaacaac
     4021 cgctattgat ttaggtttta tgcctactat gattatcaaa tctgataaag gttatcaagc
     4081 atattttgtt ttagaaacgc cagtctatgt gacttcaaaa tcagaattta aatctgtcaa
     4141 agcagccaaa ataatttcgc aaaatatccg agaatatttt ggaaagtctt tgccagttga
     4201 tctaacgtgt aatcattttg gtattgctcg cataccaaga acggacaatg tagaattttt
     4261 tgatcctaat taccgttatt ctttcaaaga atggcaagat tggtctttca aacaaacaga
     4321 taataagggc tttactcgtt caagtctaac ggttttaagc ggtacagaag gcaaaaaaca
     4381 agtagatgaa ccctggttta atctcttatt gcacgaaacg aaattttcag gagaaaaggg
     4441 tttaataggg cgtaataacg tcatgtttac cctctcttta gcctacttta gttcaggcta
     4501 ttcaatcgaa acgtgcgaat ataatatgtt tgagtttaat aatcgattag atcaaccctt
     4561 agaagaaaaa gaagtaatca aaattgttag aagtgcctat tcagaaaact atcaaggggc
     4621 taatagggaa tacattacca ttctttgcaa agcttgggta tcaagtgatt taaccagtaa
     4681 agatttattt gtccgtcaag ggtggtttaa attcaagaaa aaaagaagcg aacgtcaacg
     4741 tgttcatttg tcagaatgga aagaagattt aatggcttat attagcgaaa aaagcgatgt
     4801 atacaagcct tatttagtga cgaccaaaaa agagattaga gaagtgctag gcattcctga
     4861 acggacatta gataaattgc tgaaggtact gaaggcgaat caggaaattt tctttaagat
     4921 taaaccagga agaaatggtg gcattcaact tgctagtgtt aaatcattgt tgctatcgat
     4981 cattaaagta aaaaaagaag aaaaagaaag ctatataaag gcgctgacaa attcttttga
     5041 cttagagcat acattcattc aagagacttt aaacaagcta gcagaacgcc ctaaaacgga
     5101 cacacaactc gatttgttta gctatgatac aggctgaaaa taaaacccgc actatgccat
     5161 tacatttata tctatgatac gtgtttgttt tttctttgct gtttagcgaa tgattagcag
     5221 aaatatacag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
     5281 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
     5341 gtgagagcga agcgaacact tgatttttta attttctatc ttttataggt cattagagta
     5401 tacttatttg tcctataaac tatttagcag cataatagat ttattgaata ggtcatttaa
     5461 gttgagcata ttagaggagg aaaatcttgg agaaatattt gaagaacccg attacatgga
     5521 ttggattagt tcttgtggtt acgtggtttt taactaaaag tagtgaattt ttgatttttg
     5581 gtgtgtgtgt cttgttgtta gtatttgcta gtcaaagtga ttaaatagaa ttccggatga
     5641 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     5701 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     5761 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     5821 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     5881 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     5941 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     6001 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     6061 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     6121 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     6181 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     6241 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     6301 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     6361 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     6421 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     6481 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     6541 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     6601 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     6661 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     6721 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6781 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     6841 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     6901 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     6961 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     7021 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     7081 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg tttgacagct
     7141 tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     7201 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     7261 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     7321 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     7381 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     7441 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     7501 ccttatttgt atagttcatc catgccatgt gtaatcccag cagccgttac aaactcaaga
     7561 aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg tgtggacagg
     7621 taatggttgt ctggtaaaag gacagggtca tcgccaattg gagtattttg ttgataatga
     7681 tcagcgagtt gcacgccgcc gtcttcgatg ttgtggcggg tcttgaagtt ggctttgatg
     7741 ccgttctttt gcttgtcggc catgatgtat acgttgtggg agttgtagtt gtattccaac
     7801 ttgtggccga ggatgtttcc gtcctccttg aaatcgattc ccttaagctc gatcctgttg
     7861 acgagggtgt ctccctcaaa cttgacttca gcacgtgtct tgtagttccc gtcgtccttg
     7921 aagaagatgg tcctctcctg cacgtatccc tcaggcatgg cgctcttgaa gaagtcgtgc
     7981 cgcttcatat gatctggata tcttgaaaag cattgaacac cataagtgag agtagtgaca
     8041 agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag ggtaagtttt
     8101 ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtaccc attaacatca
     8161 ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc tttactcatg
     8221 aattcaagtc tcctttttta ttagtgataa ttttaacaaa gaaaattata ccatgttgaa
     8281 gagcattaat aaaattatta ttttgtgttt gtgctattat agttgagatt attattaatg
     8341 aggggtaaat aagatgaaga taattgcagg tttgggtaat ccgggtcaaa aatatgataa
     8401 gaccaaacat aatactggtt tcatgacaat ggatcactac cttgataaaa aaggtttgac
     8461 tttaaataaa gataaatttg aagggcattg gactaaaaag cttatcgata ccg
//
