LOCUS       40924_44157        4392 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 4392)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2001) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      47..76
                     /label=Ipp
                     /note="Ipp"
                     /regulatory_class="promoter"
     promoter        119..149
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    157..173
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    243..992
                     /label=RNAP alpha subunit
                     /note="RNAP alpha subunit"
     primer_bind     957..974
                     /label=forward
                     /note="forward"
     misc_feature    993..1058
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1102..1119)
                     /label=reverse
                     /note="reverse"
     rep_origin      1274..1862
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2048..2188)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2293..2481)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3123..4310)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(4358..4386)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 gaattgagcg aacgtcaaaa ataagtgcct tcccatcaaa aaaatattga caacataaaa
       61 aactttgtgt tatacttgta acgctacatg gagattaact caatctagct agagaggctt
      121 tacactttat gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacggat tcactggaac tctagaccaa agagaggaca
      241 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
      301 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
      361 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
      421 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
      481 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
      541 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
      601 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
      661 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgagttc
      721 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
      781 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
      841 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
      901 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
      961 ctgaagtgaa agaagagaaa ccagaggcgg ccggatccgc ggccgcaaga attcagtctg
     1021 agctggcgct cgagtaatta attaattaat gaactagtga gatcctctag agtcgacctg
     1081 caggcatgca agcttggcag tttatggcgg gcgacgaatc aggtggcact tttcggggaa
     1141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1381 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1981 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2041 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     2101 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     2161 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     2221 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2281 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     2341 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     2401 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     2461 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     2521 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     2581 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     2641 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     2701 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     2761 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     2821 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     2881 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     2941 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc
     3001 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     3061 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     3121 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     3181 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     3241 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     3301 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     3361 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     3421 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
     3481 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     3541 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     3601 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     3661 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     3721 aaggctctca agggcatggg tcggcgctct cccttatgcg actcctgcat taggaagcag
     3781 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgtaaggag
     3841 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     3901 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     3961 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagagaatc
     4021 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
     4081 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga
     4141 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa
     4201 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
     4261 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
     4321 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctaatc gatgataagc
     4381 tgtcaaacat ga
//
