LOCUS       62056_21445        4653 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        119..149
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    157..173
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1535..2123
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2309..2449)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2554..2742)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3384..4571)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(4619..4647)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 gaattgagcg aacgtcaaaa ataagtgcct tcccatcaaa aaaatattga caacataaaa
       61 aactttgtgt tatacttgta acgctacatg gagattaact caatctagct agagaggctt
      121 tacactttat gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacggat tcactggaac tctagaccaa agagaggaca
      241 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
      301 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
      361 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
      421 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
      481 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
      541 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
      601 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
      661 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgagttc
      721 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
      781 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
      841 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
      901 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
      961 ctgaagtgaa agaagagaaa ccagaggcgg ccggatccgc ggccgcaaga attcagcctc
     1021 aacagcagca aatgcaacct cccaattcaa gtgcggacaa caaccctttg caacagcaat
     1081 catcacaaaa taccgtacca aacgtcctca accaaattaa ccaaatcttt tctccagagg
     1141 agcaacgcag cttattacaa gaagccatcg aaacctgcaa gaattttgaa aaaacacaat
     1201 tgggtagtac gatgacggaa cctgtcaagc aaagttttat taggaaatac attgtgcaaa
     1261 aggccctgag aaaaatccaa gctttgtgac tcgagtaatt aattaattaa tgaactagtg
     1321 agatcctcta gagtcgacct gcaggcatgc aagcttggca gtttatggcg ggcgacgaat
     1381 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1441 attcaaatat gtatccgctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1501 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1561 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1621 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1681 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1741 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1801 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1861 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1921 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1981 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2041 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2101 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2161 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2221 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2281 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2341 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2401 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2461 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2521 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2581 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     2641 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     2701 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     2761 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     2821 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     2881 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     2941 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     3001 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     3061 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     3121 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     3181 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
     3241 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     3301 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     3361 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     3421 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     3481 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
     3541 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     3601 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     3661 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     3721 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     3781 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     3841 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     3901 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     3961 cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc tcccttatgc
     4021 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     4081 aggaatggtg catgtaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     4141 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     4201 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     4261 atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
     4321 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     4381 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
     4441 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
     4501 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     4561 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
     4621 taaagctaat cgatgataag ctgtcaaaca tga
//
