LOCUS       40924_44132        6098 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTREXL-ble, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6098)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..811
                     /gene="ribosomal promoter"
                     /regulatory_class="promoter"
     gene            2..811
                     /gene="ribosomal promoter"
                     /label=ribosomal promoter
     intron          862..1033
                     /gene="HX1"
     gene            862..1033
                     /gene="HX1"
                     /label=HX1
     CDS             1063..1779
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1819..2331
                     /gene="IgapdhI-II"
     gene            1819..2331
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             2332..2703
                     /label=BleoR
                     /note="antibiotic-binding protein"
     intron          2707..3224
                     /gene="IgapdhII"
     gene            2707..3224
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3261..3279)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3300..3316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3324..3340)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3348..3378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3393..3414)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3702..4290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4464..5321)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5322..5426)
                     /label=AmpR promoter
     rep_origin      complement(5452..5907)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6049..6065
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6072..6090
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcggtg caccctggga tatttgcgca cccacctttc ccgtcgcaat cggcgccgtc
       61 cgaacgcgga aatgttccga gaataatacg gctgggctgc gccgcgcgtt ccccgcgtga
      121 accaactatt gtgcgatgtg cagcatccta ttgtggtgct ttaccgcaca ccagtttcct
      181 tgcggggctt atttggtgtg ttggattttt aattttttga tgattcggag gaattttttg
      241 caatttttgg gtacccctaa aaaaattttg gagtttccgg ggttcgatcc aacttgtgct
      301 tttttaaccg tccatttgtg tctagtacat catggagcgg tattctctcc ggtggcccag
      361 gtggcgtcgg aaggaatcgg tgtccctcag gatgtttggt ttttcgtttt tttttttgcg
      421 gtcttttgtt tcgcatcgcc gtatcgtgtg cggcgcgacc ttctttttgg ctcattggtc
      481 agtgaggttt ataataaagt ggatcatttg ggtttttggc cctttgcggc gcacccgcgg
      541 aatgggggag ggattttcat ataattgtag tgtacacatt gtgtgtgggt cgcaaaatgt
      601 catgccgtgt gcaatgttta tttgcgtgct gtgcgtgtcc ttttgtgatt ccaccacttg
      661 tgtgtatttt accttatcgt ctctccctgc cgttggctgt gcgtgaattg tgcttcgcat
      721 tcgtgggaaa tcaaaagggg aatcaacgtg ttcttttttt ttcagaaggg gagtcaaagc
      781 gccgtgtgga tgccaagtct gtgcaagagt tctagtggat cccccaacga gtttcttcaa
      841 aatatgcagc ggattcacta agaaacattt tcacgcacga aagcgaaatt attatgattg
      901 ttattataat actttttctt tgttgtttta tccacttatt attgttgtgt taaattgttt
      961 ttaccttttt tcttttccaa cttcttttat gatgtctttt cttttttttt tttgctctat
     1021 aagttgtctt gtctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag
     1081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1201 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1261 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1741 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1801 attcgatatc aagcttaggc gtggcgatga cttcaggtct ttcttttgcg aatagggatc
     1861 ttataataca cgatgcgtgt cccgtgatga tcgttaccgg tgctgccacg atccaactga
     1921 cacagcgtca agagcaaaac aattttactt ttccctttaa ggacaacaac aaaaaaatat
     1981 ataacttttt tttctttttt tttttttttg aaattatatt tatggtcatc tttgggaaac
     2041 aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat caatgtacga
     2101 ggatttgggg tattgcaagg aaaatgtaga tgatttaatt gggtgtgtga tgcagcttgt
     2161 ggtaattttt gctcacttcc ctttttgcca catcttttta gtttttctgc ttttctttcc
     2221 ccattattcc acttgtctct cttttcccac gtttcctgca cgaatgcaga aagtgatatt
     2281 tttactttga aagccatcta ccaacaacaa ttacattgaa cagaatttaa aatggccaag
     2341 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     2401 accgaccggc tcgggttctc ccgggacttc gtggagaacg acttcgccgg tgtggtccgg
     2461 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     2521 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     2581 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     2641 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     2701 gactaggcgt ggcgatgact tcaggtcttt cttttgcgaa tagggatctt ataatacacg
     2761 atgcgtgtcc cgtgatggtc gttaccggtg ctgccacgat ccaagtgaca cagtgtcaag
     2821 agcaaaacaa ttttactttt ccctttaagg acaacaacaa aaaaatatat aacttttttt
     2881 tctttttttt ttttgaaatt atatttatgg tcatctttgg gaaacaaaaa gcagcaattt
     2941 aatgatgcgg aaggatgagt gaaataatgt ttaatcaatg tacgaggatt tggggtattg
     3001 caaggaaaat gtagatgatt tgattgggtg tatgatgcag cttgtggtaa ttttttacaa
     3061 ctttgcgtta ttggaagatt ttggttgttc agtttctttt tgtttataac tgactgtttt
     3121 aatttagtgt gatttttttt tttaaggatt taagctgttt ttcttttttt ttctttttct
     3181 tcactgatgg tgtttaaggt gtagtttagt gtttgtgcat tatgcatgca agctagccgt
     3241 gtgggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     3301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4141 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     5461 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5521 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     5581 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5641 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5701 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5761 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5821 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5881 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     5941 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     6001 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     6061 ccagtgaatt gtaatacgac tcactatagg gcgaattg
//
