LOCUS       40924_44152        6323 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTREXL-PAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6323)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..811
                     /gene="ribosomal promoter"
                     /regulatory_class="promoter"
     gene            2..811
                     /gene="ribosomal promoter"
                     /label=ribosomal promoter
     intron          862..1033
                     /gene="HX1"
     gene            862..1033
                     /gene="HX1"
                     /label=HX1
     CDS             1063..1779
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1819..2331
                     /gene="IgapdhI-II"
     gene            1819..2331
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             2332..2928
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     intron          2932..3449
                     /gene="IgapdhII"
     gene            2932..3449
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3486..3504)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3525..3541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3549..3565)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3573..3603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3618..3639)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3927..4515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4689..5546)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5547..5651)
                     /label=AmpR promoter
     rep_origin      complement(5677..6132)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6274..6290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6297..6315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcggtg caccctggga tatttgcgca cccacctttc ccgtcgcaat cggcgccgtc
       61 cgaacgcgga aatgttccga gaataatacg gctgggctgc gccgcgcgtt ccccgcgtga
      121 accaactatt gtgcgatgtg cagcatccta ttgtggtgct ttaccgcaca ccagtttcct
      181 tgcggggctt atttggtgtg ttggattttt aattttttga tgattcggag gaattttttg
      241 caatttttgg gtacccctaa aaaaattttg gagtttccgg ggttcgatcc aacttgtgct
      301 tttttaaccg tccatttgtg tctagtacat catggagcgg tattctctcc ggtggcccag
      361 gtggcgtcgg aaggaatcgg tgtccctcag gatgtttggt ttttcgtttt tttttttgcg
      421 gtcttttgtt tcgcatcgcc gtatcgtgtg cggcgcgacc ttctttttgg ctcattggtc
      481 agtgaggttt ataataaagt ggatcatttg ggtttttggc cctttgcggc gcacccgcgg
      541 aatgggggag ggattttcat ataattgtag tgtacacatt gtgtgtgggt cgcaaaatgt
      601 catgccgtgt gcaatgttta tttgcgtgct gtgcgtgtcc ttttgtgatt ccaccacttg
      661 tgtgtatttt accttatcgt ctctccctgc cgttggctgt gcgtgaattg tgcttcgcat
      721 tcgtgggaaa tcaaaagggg aatcaacgtg ttcttttttt ttcagaaggg gagtcaaagc
      781 gccgtgtgga tgccaagtct gtgcaagagt tctagtggat cccccaacga gtttcttcaa
      841 aatatgcagc ggattcacta agaaacattt tcacgcacga aagcgaaatt attatgattg
      901 ttattataat actttttctt tgttgtttta tccacttatt attgttgtgt taaattgttt
      961 ttaccttttt tcttttccaa cttcttttat gatgtctttt cttttttttt tttgctctat
     1021 aagttgtctt gtctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag
     1081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1201 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1261 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1741 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1801 attcgatatc aagcttaggc gtggcgatga cttcaggtct ttcttttgcg aatagggatc
     1861 ttataataca cgatgcgtgt cccgtgatga tcgttaccgg tgctgccacg atccaactga
     1921 cacagcgtca agagcaaaac aattttactt ttccctttaa ggacaacaac aaaaaaatat
     1981 ataacttttt tttctttttt tttttttttg aaattatatt tatggtcatc tttgggaaac
     2041 aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat caatgtacga
     2101 ggatttgggg tattgcaagg aaaatgtaga tgatttaatt gggtgtgtga tgcagcttgt
     2161 ggtaattttt gctcacttcc ctttttgcca catcttttta gtttttctgc ttttctttcc
     2221 ccattattcc acttgtctct cttttcccac gtttcctgca cgaatgcaga aagtgatatt
     2281 tttactttga aagccatcta ccaacaacaa ttacattgaa cagaatttaa aatgaccgag
     2341 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     2401 gcagccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     2461 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     2521 gtgtgggtcg cggacgacgg cgccgcggtg gaggtctgga ccacgccgga gagcgtcgaa
     2581 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     2641 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     2701 ttcctggcca ccgtcggcgt ctcgcccgaa caccagggca agggtctggg cagcgccgtc
     2761 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     2821 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     2881 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta ggcgtggcga
     2941 tgacttcagg tctttctttt gcgaataggg atcttataat acacgatgcg tgtcccgtga
     3001 tggtcgttac cggtgctgcc acgatccaag tgacacagtg tcaagagcaa aacaatttta
     3061 cttttccctt taaggacaac aacaaaaaaa tatataactt ttttttcttt tttttttttg
     3121 aaattatatt tatggtcatc tttgggaaac aaaaagcagc aatttaatga tgcggaagga
     3181 tgagtgaaat aatgtttaat caatgtacga ggatttgggg tattgcaagg aaaatgtaga
     3241 tgatttgatt gggtgtatga tgcagcttgt ggtaattttt tacaactttg cgttattgga
     3301 agattttggt tgttcagttt ctttttgttt ataactgact gttttaattt agtgtgattt
     3361 ttttttttaa ggatttaagc tgtttttctt tttttttctt tttcttcact gatggtgttt
     3421 aaggtgtagt ttagtgtttg tgcattatgc atgcaagcta gccgtgtggg taccagcttt
     3481 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     3541 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3601 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3661 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3721 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5641 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     5701 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5761 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5821 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5881 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5941 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6001 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6061 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6121 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6181 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6241 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     6301 acgactcact atagggcgaa ttg
//
