LOCUS       40924_44142        6749 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTREXL-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..811
                     /gene="ribosomal promoter"
                     /regulatory_class="promoter"
     gene            2..811
                     /gene="ribosomal promoter"
                     /label=ribosomal promoter
     intron          862..1033
                     /gene="HX1"
     gene            862..1033
                     /gene="HX1"
                     /label=HX1
     CDS             1063..1779
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1819..2331
                     /gene="IgapdhI-II"
     gene            1819..2331
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             2332..3354
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          3358..3875
                     /gene="IgapdhII"
     gene            3358..3875
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3912..3930)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3951..3967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3975..3991)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3999..4029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4044..4065)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4353..4941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5115..5972)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5973..6077)
                     /label=AmpR promoter
     rep_origin      complement(6103..6558)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6700..6716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6723..6741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcggtg caccctggga tatttgcgca cccacctttc ccgtcgcaat cggcgccgtc
       61 cgaacgcgga aatgttccga gaataatacg gctgggctgc gccgcgcgtt ccccgcgtga
      121 accaactatt gtgcgatgtg cagcatccta ttgtggtgct ttaccgcaca ccagtttcct
      181 tgcggggctt atttggtgtg ttggattttt aattttttga tgattcggag gaattttttg
      241 caatttttgg gtacccctaa aaaaattttg gagtttccgg ggttcgatcc aacttgtgct
      301 tttttaaccg tccatttgtg tctagtacat catggagcgg tattctctcc ggtggcccag
      361 gtggcgtcgg aaggaatcgg tgtccctcag gatgtttggt ttttcgtttt tttttttgcg
      421 gtcttttgtt tcgcatcgcc gtatcgtgtg cggcgcgacc ttctttttgg ctcattggtc
      481 agtgaggttt ataataaagt ggatcatttg ggtttttggc cctttgcggc gcacccgcgg
      541 aatgggggag ggattttcat ataattgtag tgtacacatt gtgtgtgggt cgcaaaatgt
      601 catgccgtgt gcaatgttta tttgcgtgct gtgcgtgtcc ttttgtgatt ccaccacttg
      661 tgtgtatttt accttatcgt ctctccctgc cgttggctgt gcgtgaattg tgcttcgcat
      721 tcgtgggaaa tcaaaagggg aatcaacgtg ttcttttttt ttcagaaggg gagtcaaagc
      781 gccgtgtgga tgccaagtct gtgcaagagt tctagtggat cccccaacga gtttcttcaa
      841 aatatgcagc ggattcacta agaaacattt tcacgcacga aagcgaaatt attatgattg
      901 ttattataat actttttctt tgttgtttta tccacttatt attgttgtgt taaattgttt
      961 ttaccttttt tcttttccaa cttcttttat gatgtctttt cttttttttt tttgctctat
     1021 aagttgtctt gtctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag
     1081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1201 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1261 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1741 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1801 attcgatatc aagcttaggc gtggcgatga cttcaggtct ttcttttgcg aatagggatc
     1861 ttataataca cgatgcgtgt cccgtgatga tcgttaccgg tgctgccacg atccaactga
     1921 cacagcgtca agagcaaaac aattttactt ttccctttaa ggacaacaac aaaaaaatat
     1981 ataacttttt tttctttttt tttttttttg aaattatatt tatggtcatc tttgggaaac
     2041 aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat caatgtacga
     2101 ggatttgggg tattgcaagg aaaatgtaga tgatttaatt gggtgtgtga tgcagcttgt
     2161 ggtaattttt gctcacttcc ctttttgcca catcttttta gtttttctgc ttttctttcc
     2221 ccattattcc acttgtctct cttttcccac gtttcctgca cgaatgcaga aagtgatatt
     2281 tttactttga aagccatcta ccaacaacaa ttacattgaa cagaatttaa aatgaaaaag
     2341 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     2401 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     2461 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     2521 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     2581 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     2641 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     2701 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     2761 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     2821 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     2881 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     2941 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     3001 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     3061 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     3121 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     3181 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     3241 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     3301 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggactaggcg
     3361 tggcgatgac ttcaggtctt tcttttgcga atagggatct tataatacac gatgcgtgtc
     3421 ccgtgatggt cgttaccggt gctgccacga tccaagtgac acagtgtcaa gagcaaaaca
     3481 attttacttt tccctttaag gacaacaaca aaaaaatata taactttttt ttcttttttt
     3541 tttttgaaat tatatttatg gtcatctttg ggaaacaaaa agcagcaatt taatgatgcg
     3601 gaaggatgag tgaaataatg tttaatcaat gtacgaggat ttggggtatt gcaaggaaaa
     3661 tgtagatgat ttgattgggt gtatgatgca gcttgtggta attttttaca actttgcgtt
     3721 attggaagat tttggttgtt cagtttcttt ttgtttataa ctgactgttt taatttagtg
     3781 tgattttttt ttttaaggat ttaagctgtt tttctttttt tttctttttc ttcactgatg
     3841 gtgtttaagg tgtagtttag tgtttgtgca ttatgcatgc aagctagccg tgtgggtacc
     3901 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
     3961 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4021 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4081 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4141 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4201 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4981 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5041 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5101 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5161 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5221 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5281 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5341 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5401 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5461 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5521 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5581 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5641 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5701 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5761 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5821 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5881 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5941 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6001 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6061 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6121 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6181 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6241 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6301 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6361 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6421 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6481 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6541 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6601 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6661 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     6721 tgtaatacga ctcactatag ggcgaattg
//
