LOCUS       40924_44137        6122 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTREXL-BSD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6122)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6122)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..811
                     /gene="ribosomal promoter"
                     /regulatory_class="promoter"
     gene            2..811
                     /gene="ribosomal promoter"
                     /label=ribosomal promoter
     intron          862..1033
                     /gene="HX1"
     gene            862..1033
                     /gene="HX1"
                     /label=HX1
     CDS             1063..1779
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1819..2331
                     /gene="IgapdhI-II"
     gene            1819..2331
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             2332..2727
                     /label=BSD
                     /note="blasticidin S deaminase"
     intron          2731..3248
                     /gene="IgapdhII"
     gene            2731..3248
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3285..3303)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3324..3340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3348..3364)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3372..3402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3417..3438)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3726..4314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4488..5345)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5346..5450)
                     /label=AmpR promoter
     rep_origin      complement(5476..5931)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6073..6089
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6096..6114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcggtg caccctggga tatttgcgca cccacctttc ccgtcgcaat cggcgccgtc
       61 cgaacgcgga aatgttccga gaataatacg gctgggctgc gccgcgcgtt ccccgcgtga
      121 accaactatt gtgcgatgtg cagcatccta ttgtggtgct ttaccgcaca ccagtttcct
      181 tgcggggctt atttggtgtg ttggattttt aattttttga tgattcggag gaattttttg
      241 caatttttgg gtacccctaa aaaaattttg gagtttccgg ggttcgatcc aacttgtgct
      301 tttttaaccg tccatttgtg tctagtacat catggagcgg tattctctcc ggtggcccag
      361 gtggcgtcgg aaggaatcgg tgtccctcag gatgtttggt ttttcgtttt tttttttgcg
      421 gtcttttgtt tcgcatcgcc gtatcgtgtg cggcgcgacc ttctttttgg ctcattggtc
      481 agtgaggttt ataataaagt ggatcatttg ggtttttggc cctttgcggc gcacccgcgg
      541 aatgggggag ggattttcat ataattgtag tgtacacatt gtgtgtgggt cgcaaaatgt
      601 catgccgtgt gcaatgttta tttgcgtgct gtgcgtgtcc ttttgtgatt ccaccacttg
      661 tgtgtatttt accttatcgt ctctccctgc cgttggctgt gcgtgaattg tgcttcgcat
      721 tcgtgggaaa tcaaaagggg aatcaacgtg ttcttttttt ttcagaaggg gagtcaaagc
      781 gccgtgtgga tgccaagtct gtgcaagagt tctagtggat cccccaacga gtttcttcaa
      841 aatatgcagc ggattcacta agaaacattt tcacgcacga aagcgaaatt attatgattg
      901 ttattataat actttttctt tgttgtttta tccacttatt attgttgtgt taaattgttt
      961 ttaccttttt tcttttccaa cttcttttat gatgtctttt cttttttttt tttgctctat
     1021 aagttgtctt gtctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag
     1081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1201 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1261 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1741 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1801 attcgatatc aagcttaggc gtggcgatga cttcaggtct ttcttttgcg aatagggatc
     1861 ttataataca cgatgcgtgt cccgtgatga tcgttaccgg tgctgccacg atccaactga
     1921 cacagcgtca agagcaaaac aattttactt ttccctttaa ggacaacaac aaaaaaatat
     1981 ataacttttt tttctttttt tttttttttg aaattatatt tatggtcatc tttgggaaac
     2041 aaaaagcagc aatttaatga tgcggaagga tgagtgaaat aatgtttaat caatgtacga
     2101 ggatttgggg tattgcaagg aaaatgtaga tgatttaatt gggtgtgtga tgcagcttgt
     2161 ggtaattttt gctcacttcc ctttttgcca catcttttta gtttttctgc ttttctttcc
     2221 ccattattcc acttgtctct cttttcccac gtttcctgca cgaatgcaga aagtgatatt
     2281 tttactttga aagccatcta ccaacaacaa ttacattgaa cagaatttaa aatggccaag
     2341 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     2401 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     2461 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     2521 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     2581 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     2641 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaactg
     2701 ctgccctctg gttatgtgtg ggagggctag gcgtggcgat gacttcaggt ctttcttttg
     2761 cgaataggga tcttataata cacgatgcgt gtcccgtgat ggtcgttacc ggtgctgcca
     2821 cgatccaagt gacacagtgt caagagcaaa acaattttac ttttcccttt aaggacaaca
     2881 acaaaaaaat atataacttt tttttctttt ttttttttga aattatattt atggtcatct
     2941 ttgggaaaca aaaagcagca atttaatgat gcggaaggat gagtgaaata atgtttaatc
     3001 aatgtacgag gatttggggt attgcaagga aaatgtagat gatttgattg ggtgtatgat
     3061 gcagcttgtg gtaatttttt acaactttgc gttattggaa gattttggtt gttcagtttc
     3121 tttttgttta taactgactg ttttaattta gtgtgatttt tttttttaag gatttaagct
     3181 gtttttcttt ttttttcttt ttcttcactg atggtgttta aggtgtagtt tagtgtttgt
     3241 gcattatgca tgcaagctag ccgtgtgggt accagctttt gttcccttta gtgagggtta
     3301 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3361 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3421 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3481 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3541 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4141 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5461 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     5521 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5581 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5641 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5701 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5761 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5821 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     5881 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     5941 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     6001 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     6061 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     6121 tg
//
