LOCUS       pTRE3G.        3431 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of a gene in cells 
            containing the Tet-On(R) 3G transactivator protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     misc_feature    383..452
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          583..648
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             778..798
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1223..1344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1601..2189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2363..3220)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3221..3325)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
       61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
      361 taccacttcc taccctcgta aagtcgacac cggggcccag atctatcgat cggccggata
      421 tcacgcgtca tatggctagc ctgcagggat ccaatgtaac tgtattcagc gatgacgaaa
      481 ttcttagcta ttgtaatact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg
      541 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
      601 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      661 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      721 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      781 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      841 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      901 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      961 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     1021 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     1081 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     1141 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     1201 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1321 gtccaaactc atcaatgtat cttatcatgt ctgcggctct agagctgcat taatgaatcg
     1381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3361 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     3421 ttcaagaatt c
//
