LOCUS       pTRE3G-mCherry.        4715 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of a gene together with 
            the mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     CDS             389..1096
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1107..1694
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1701..1736
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          1867..1932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2062..2082
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2507..2628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2885..3473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3647..4504)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4505..4609)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
       61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
      361 taccacttcc taccctcgta aagtcgacat ggtgagcaag ggcgaggagg ataacatggc
      421 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      481 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      541 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      601 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      661 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      721 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      781 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      841 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      901 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      961 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1021 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1081 ggacgagctg tacaagtagc ggccggcccc tctccctccc ccccccccta acgttactgg
     1141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     1201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     1261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     1321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     1441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     1501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     1561 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa
     1621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     1681 atatggccac aaccgggccg gatatcacgc gtcatatggc tagcctgcag ggatccaatg
     1741 taactgtatt cagcgatgac gaaattctta gctattgtaa tactctagag gatctttgtg
     1801 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     1861 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     1921 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     1981 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     2041 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     2101 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     2161 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     2221 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     2281 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     2341 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     2401 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     2461 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2521 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2581 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg
     2641 ctctagagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2701 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3541 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3601 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3661 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3721 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3781 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3841 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3901 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3961 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4021 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4081 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4141 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4201 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4261 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4321 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4381 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4441 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4501 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4561 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4621 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4681 ggcgtatcac gaggcccttt cgtcttcaag aattc
//
