LOCUS       pTRE3G-ZsGreen1.        4700 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of a gene together with 
            the ZsGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-ZsGreen1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid actually contains ZsGreen with its original codons 
            rather than the mammalian codon-optimized ZsGreen1.
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     CDS             389..1081
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     misc_feature    1092..1679
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1686..1721
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          1852..1917
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2047..2067
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2492..2613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2870..3458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3632..4489)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4490..4594)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
       61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
      361 taccacttcc taccctcgta aagtcgacat ggctcagtca aagcacggtc taacaaaaga
      421 aatgacaatg aaataccgta tggaagggtg cgtcgatgga cataaatttg tgatcacggg
      481 agagggcatt ggatatccgt tcaaagggaa acaggctatt aatctgtgtg tggtcgaagg
      541 tggaccattg ccatttgccg aagacatatt gtcagctgcc tttatgtacg gaaacagggt
      601 tttcactgaa tatcctcaag acatagctga ctatttcaag aactcgtgtc ctgctggtta
      661 tacatgggac aggtcttttc tctttgagga tggagcagtt tgcatatgta atgcagatat
      721 aacagtgagt gttgaagaaa actgcatgta tcatgagtcc aaattttatg gagtgaattt
      781 tcctgctgat ggacctgtga tgaaaaagat gacagataac tgggagccat cctgcgagaa
      841 gatcatacca gtacctaagc aggggatatt gaaaggggat gtctccatgt acctccttct
      901 gaaggatggt gggcgtttac ggtgccaatt cgacacagtt tacaaagcaa agtctgtgcc
      961 aagaaagatg ccggactggc acttcatcca gcataagctc acccgtgaag accgcagcga
     1021 tgctaagaat cagaaatggc atctgacaga acatgctatt gcatccggat ctgcattgcc
     1081 ctgacggccg gcccctctcc ctcccccccc ccctaacgtt actggccgaa gccgcttgga
     1141 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     1201 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     1261 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     1321 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     1381 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     1441 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     1501 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     1561 ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     1621 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaaccg
     1681 ggccggatat cacgcgtcat atggctagcc tgcagggatc caatgtaact gtattcagcg
     1741 atgacgaaat tcttagctat tgtaatactc tagaggatct ttgtgaagga accttacttc
     1801 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     1861 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     1921 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     1981 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     2041 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     2101 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     2161 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     2221 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     2281 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     2341 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     2401 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2461 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgcggctcta gagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4681 cctttcgtct tcaagaattc
//
