LOCUS       pTRE3G-Hom1.        3775 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer
            domain, for creating soluble fusion proteins that can be dimerized 
            with a drug.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-Hom1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     misc_feature    383..422
                     /label=5' MCS
                     /note="5' MCS"
                     /note="multiple cloning site upstream of DmrB"
     CDS             423..425
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             435..755
                     /label=DmrB
                     /note="F36V mutant of FK506-binding protein FKBP12"
     misc_feature    756..785
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          927..992
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1122..1142
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1567..1688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1945..2533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2707..3564)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3565..3669)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
       61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
      361 taccacttcc taccctcgta aagtcgacac cggggcccag atctatcgat cggccggata
      421 tcatggcttc tagaggagtg caggtggaga ctatctcccc aggagacggg cgcaccttcc
      481 ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaag
      541 ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
      601 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
      661 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
      721 ctctcgtctt cgatgtggag cttctaaaac tggaaacgcg tcatatggct agcctgcagg
      781 gatcctgact gactgaaatg taactgtatt cagcgatgac gaaattctta gctattgtaa
      841 tactctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      901 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      961 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     1021 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1081 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1141 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1201 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1261 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     1321 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     1381 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     1441 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     1501 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1561 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1621 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1681 gtatcttatc atgtctgcgg ctctagagct gcattaatga atcggccaac gcgcggggag
     1741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3721 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattc
//
