LOCUS       pTRE3G-BI-Het1.        3524 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for doxycycline-inducible dual expression of FKBP- 
            and FRB-tagged proteins, allowing drug-inducible heterodimerization.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-BI-Het1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On(R) 3G"
     misc_feature    452..485
                     /label=5' MCS-1
                     /note="5' MCS-1"
                     /note="multiple cloning site upstream of DmrA"
     CDS             486..488
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             495..815
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     misc_feature    816..831
                     /label=3' MCS-1
                     /note="3' MCS-1"
                     /note="multiple cloning site downstream of DmrA"
     polyA_signal    967..1048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1230..1818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1992..2849)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2850..2954)
                     /label=AmpR promoter
     polyA_signal    complement(2987..3068)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3199..3210
                     /label=3' MCS-2
                     /note="3' MCS-2"
                     /note="multiple cloning site downstream of DmrC"
     CDS             complement(3211..3489)
                     /label=FRB
                     /note="FKBP-rapamycin binding domain of human FRAP"
     CDS             complement(3499..3501)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    3502..3519
                     /label=5' MCS-2
                     /note="5' MCS-2"
                     /note="multiple cloning site upstream of DmrC"
ORIGIN
        1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
       61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
      121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
      181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
      421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctccgcggg
      481 gatccatgtc tagaggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc
      541 ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat
      601 ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
      661 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
      721 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
      781 ctctcgtctt cgatgtggag cttctaaaac tggaaatcga tccgcggccg ccggcgtgac
      841 tgactgagat atctccagag gatcataatc agccatacca catttgtaga ggttttactt
      901 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      961 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1021 ttcacaaata aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc
     1081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1141 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1201 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1261 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1321 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1381 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1441 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1501 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1561 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1621 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1681 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1741 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1801 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1861 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1921 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1981 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2041 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2101 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2161 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2221 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2281 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2341 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2401 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2461 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2521 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2581 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2641 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2701 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2761 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2821 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2941 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat
     3001 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3061 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     3121 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     3181 acatatgtca gtcagtcact gcagacgcgt ctttgagatt cgtcggaaca catgataata
     3241 gaggtcccag gcttggagga ggtccttgac attccctgat ttcatgtact tcctgcacca
     3301 ctcttgggcc tccattaaat ctcgaccata ggcctgatta aaggatgttt ccttcagagt
     3361 ctgggggccc cgttccatca tagcatgcaa gggctccagc acctcaaaca tgcctttcac
     3421 gttcctttcc ccaaagtaca aacgagatgc ctcttccagg ccttcatgcc acatctcatg
     3481 ccagaggatt ctagaagcca tggtaccccc ggggaattct ccag
//
