LOCUS       V012830                 6966 bp    DNA     circular SYN 13-DEC-2023
DEFINITION  Exported.
ACCESSION   V012830
VERSION     V012830
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             480..2129
                     /label="luciferase"
                     /note="firefly luciferase"
     intron          2195..2767
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2771..2893
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2964..3019
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3581..4169)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4343..5200)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5201..5305)
                     /label="AmpR promoter"
     polyA_signal    complement(5406..5487)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(5607..6629)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(join(6638..6966,1))
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatccacca
      481 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgcta gaggatggaa
      541 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      601 cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg
      661 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      721 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      781 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt
      841 cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
      901 aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat taccagggat
      961 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     1021 ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaac tcctctggat
     1081 ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc
     1141 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     1201 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     1261 gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca
     1321 aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga
     1381 ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga
     1441 aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg
     1501 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     1561 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     1621 cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt
     1681 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     1741 gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt
     1801 taattaaata caaaggatac caggtggccc ccgctgaatt ggagtcgata ttgttacaac
     1861 accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc
     1921 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     1981 acgtcgccag tcaagtaaca accgccaaaa agttgcgcgg aggagttgtg tttgtggacg
     2041 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     2101 aggccaagaa gggcggaaag tccaaattgt aaaatgctag cgcggccgca tcgataagct
     2161 tgtcgacgat atctctagag ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     2221 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     2281 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     2341 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     2401 ttttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact ttcgtttatt
     2461 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtaattata
     2521 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     2581 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     2641 ggtaatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     2701 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     2761 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt
     2821 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2881 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2941 ccttgagcat ctgacttctg ggtaataaag gaaatttatt ttcattgcaa tagtgtgtgg
     3001 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     3061 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa
     3121 ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3181 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3241 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3301 gactactccc agtcatagct gtccctcttc tcttatgaac tcgactgcat taatgaatcg
     3361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     3781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4021 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5341 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5401 tcgaggcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5461 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5521 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     5581 gctgattatg atcctctaga actctattcc tttgccctcg gacgagtgct ggggcgtcgg
     5641 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     5701 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     5761 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     5821 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     5881 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     5941 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     6001 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     6061 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     6121 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     6181 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     6241 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
     6301 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
     6361 caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
     6421 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     6481 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     6541 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     6601 gacgtcgcgg tgagttcagg ctttttcatg gaagcttttt gcaaaagcct aggcctccaa
     6661 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     6721 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     6781 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
     6841 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
     6901 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
     6961 gacaca
//
