LOCUS       pTRE-Tight-BI-Ds        3491 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing DsRed-Express and another gene with the 
            Tet-On(R) Advanced or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Tight-BI-DsRed-Express
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    335..405
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    519..600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1544..2401)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2402..2506)
                     /label=AmpR promoter
     polyA_signal    complement(2539..2620)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2737..3411)
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     promoter        3422..3491
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagatttcga gctcggtacc cggggatcct ctagtcagct
      361 gacgcgtgct agcgcggccg catcgataag cttgtcgacg atatctccag aggatcataa
      421 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      481 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      601 cccgagcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     2161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2521 aagtgccacc tgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2581 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2641 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     2701 caaatgtggt atggctgatt atgatcctct agactacagg aacaggtggt ggcggccctc
     2761 ggcgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     2821 ggagtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatat agatagactt
     2881 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt
     2941 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tagtcttctt
     3001 ctgcattacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa
     3061 ggagccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     3121 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
     3181 ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca
     3241 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     3301 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     3361 gcgcaccttg aagcgcatga actccttgat gacgtcctcg gaggaggcca tgaattctcc
     3421 aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc
     3481 acctcgacat a
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