LOCUS       pTRE-Tight-BI-Ac        3533 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing AcGFP1 and another gene with the Tet-On(R) 
            Advanced or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Tight-BI-AcGFP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    335..405
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    519..600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1544..2401)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2402..2506)
                     /label=AmpR promoter
     polyA_signal    complement(2539..2620)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2737..3453)
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
     promoter        3464..3533
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagatttcga gctcggtacc cggggatcct ctagtcagct
      361 gacgcgtgct agcgcggccg catcgataag cttgtcgacg atatctccag aggatcataa
      421 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      481 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      601 cccgagcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     2161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2521 aagtgccacc tgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2581 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2641 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     2701 caaatgtggt atggctgatt atgatcctct agatcacttg tacagctcat ccatgccgtg
     2761 ggtgatggcg gcggcggtca cgaagccgaa gtagatcatg tgatcgcgct tctcgttggg
     2821 gtccttggac agggcgctct gggtggacag gtagtggtta tcgggcagca gcacagggcc
     2881 atcgccgatg ggggtattct gctggtagtg gtcggccagc tgcacgctgc catcctcgat
     2941 gttgtggcgg atcttgaagt tcaccttgat gccattcttg gccttgtcgg tcatgatgta
     3001 cacattgtgg gcgttgtagt tgtactccat cttattgccc aggatgttgc catcctcctt
     3061 gaaatcggtg ccggtcagct cgatgcgatt caccagggta tcgccctcga acttcacctc
     3121 ggcgcgcgac ttgtagttgc cgtcatcctc gaagaagatg gtgcgctcct ggatgtagcc
     3181 ctcaggcatg gcgctcttga agaagtcgtg ctgcttcatg tgatcggggt agcgtgagaa
     3241 gcactgcacg ccgtagctca gggtggtcac cagggtgggc cagggcacag gcagcttgcc
     3301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     3361 gctcacgctg aacttgtggc cattcacatc gccattcagc tcgatcagga tgggcacgat
     3421 gccggtgaac agctcggcgc ccttgctcac catgaattct ccaggcgatc tgacggttca
     3481 ctaaacgagc tctgcttata taggcctccc accgtacacg ccacctcgac ata
//
