LOCUS       40924_44038       10140 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRE-TALER21-4xTarget_FF3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 10140)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    66..84
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    137..155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    173..191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    244..262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        274..312
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    352..376
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    429..3764
                     /label=similar to TALER21
                     /note="similar to TALER21"
     CDS             3774..3794
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    3840..3860
                     /label=FF3
                     /note="FF3"
     misc_feature    3866..3886
                     /label=FF3
                     /note="FF3"
     misc_feature    3892..3912
                     /label=FF3
                     /note="FF3"
     misc_feature    3918..3938
                     /label=FF3
                     /note="FF3"
     protein_bind    complement(3974..3998)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4153..4208
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4569..4585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4593..4609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4617..4647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4662..4683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4856..5692
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(5722..5740)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5761..5777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5785..5801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5809..5839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5854..5875)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6163..6751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6925..7782)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7783..7887)
                     /label=AmpR promoter
     rep_origin      complement(7913..8368)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8510..8526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8536..8554
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8569..9372
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9451..9476
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat tcgcccttca ggtccgaggt tctagacgag
       61 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
      121 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      181 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
      241 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
      301 ataagcagag ctcgtttagt gaaccgtcag atcgcaaagg gcgaattcga cccaagtttg
      361 tacaaaaaag caggctgatt accggagaat tcgtgagcaa gggcgccacc atgaccggtt
      421 tgtcgaccat tcgtccgcgc aggccaagtc ctgcccgcga gcttctgccc ggaccccaac
      481 cggatagggt tcagccgact gcagatcgtg gggtgtctgc gcctgctggc agccctctgg
      541 atggcttgcc cgctcggcgg acggtgtccc ggacccggct gccatctccc cctgcgccct
      601 cacctgcgtt ctcggcgggc agcttcagcg atctgctccg tccgttcgat ccgtcgcttc
      661 ttgatacatc gcttcttgat tcgatgcctg ccgtcggcac gccgcataca gcggctgccc
      721 cagcagagtg ggatgaggcg caatcggctc tgcgtgcagc cgatgacccg ccacccaccg
      781 tgcgtgtcgc tgtcactgcc gcgcggccgc cgcgcgccaa gccggccccg cgacggcgtg
      841 ctgcgcaacc ctccgacgct tcgccggccg cgcaggtgga tctacgcacg ctcggctaca
      901 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
      961 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
     1021 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
     1081 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
     1141 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
     1201 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
     1261 cactgacggg tgcccccctg aacctgaccc cggaccaagt ggtggctatc gccagcaaca
     1321 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     1381 atggcctgac cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc
     1441 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc
     1501 aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc
     1561 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     1621 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     1681 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc
     1741 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc
     1801 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
     1861 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     1921 tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     1981 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     2041 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     2101 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     2161 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     2221 tggctatcgc cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2281 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca
     2341 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     2401 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     2461 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     2521 aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc
     2581 tgttgccggt gctgtgccag gaccatggcc tgactccgga ccaagtggtg gctatcgcca
     2641 gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     2701 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc
     2761 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     2821 cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc
     2881 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     2941 tcgccagcaa cattggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     3001 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     3061 gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg gcgttggccg
     3121 cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct gccatggatg
     3181 cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat cgccgtattg
     3241 gcgaacgcac gtcccatcgc gttgccgact acgcgcaagt ggttcgcgtg ctggagtttt
     3301 tccagtgcca ctcccaccca gcgtacgcat ttgatgaggc catgacgcag ttcgggatga
     3361 gcaggaacgg gttggtacag ctctttcgca gagtgggcgt caccgaactc gaagcccgcg
     3421 gtggaacgct ccccccagcc tcgcagcgtt gggaccgtat cctccaggca tcagggatga
     3481 aaagggccaa accgtcccct acttcagctc aaacaccgga tcaggcgtct ttgcatgcat
     3541 tcgccgattc gctggagcgt gaccttgatg cgcccagccc aatgcacgag ggagatcaga
     3601 cgcgggcaag cagccgtaaa cggtcccgat cggatcgtgc tgtcaccggc ccctccgcac
     3661 agcaggctgt cgaggtgcgc gttcccgaac agcgcgatgc gctgcatttg cccctcagct
     3721 ggagggtaaa acgcccgcgt accaggatct ggggcggcct cccggatccc agccccaaga
     3781 agaagagaaa ggtggaggcc agcggtggcg gctcaaagct tactagttaa gcggccgcaa
     3841 acgatatggg ctgaatacaa taccaaacga tatgggctga atacaatacc aaacgatatg
     3901 ggctgaatac aataccaaac gatatgggct gaatacaata caagcttgag gtgatctaga
     3961 taactgatct accacccagc tttcttgtac aaagtggtac gcgtgaattc actcctcagg
     4021 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     4081 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     4141 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     4201 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     4261 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     4321 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     4381 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     4441 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     4501 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     4561 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4621 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4681 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     4741 gacagtacta agcttggtgc gtttttatgc ttgtagtatt gtataatgtt tttaagatcc
     4801 ttaattaata gggataacag ggtaatctcg aggtttaaac gtcgacgcgg ccgctactag
     4861 ggacaggatt ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat
     4921 attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt
     4981 tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag
     5041 aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg
     5101 cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg
     5161 gctgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag
     5221 aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc
     5281 acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag
     5341 gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa
     5401 actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc
     5461 ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc
     5521 ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt
     5581 ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct
     5641 catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgct ctccaccgcg
     5701 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     5761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6601 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     7921 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7981 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     8041 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     8101 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     8161 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     8221 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     8281 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     8341 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     8401 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8461 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     8521 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctg ctttctctga
     8581 ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac
     8641 ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc
     8701 cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc
     8761 ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct
     8821 ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt
     8881 ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat
     8941 gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac
     9001 aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg
     9061 cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc
     9121 tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat
     9181 cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac
     9241 ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta
     9301 tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc
     9361 ccacagtggg gcaagcttat taccctgtta tccctaaccg gtttaccgag ctcttattgg
     9421 ttttcaaact tcattgactg tgccaagctt ctgacctctt ctcttcctcc cacagggcct
     9481 cgagacaatt gattaacatc gatacggtac cgagttggcg cgcctgggag ctcacgggga
     9541 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     9601 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     9661 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     9721 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa
     9781 agagagattt agaatgacag gcgcgcctgg ccatacatcg atacggtacc gagttggcgc
     9841 gcctgggagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     9901 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     9961 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
    10021 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
    10081 gaaaaagctt taggctgaaa gagagattta gaatgacagg cgcgcctggt ggcgaacgat
//
