LOCUS       40924_44033        9919 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRE-TALER14-4xTarget_FF4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 9919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    66..84
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    137..155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    173..191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    244..262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        274..312
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    352..376
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    429..3560
                     /label=similar to TALER14
                     /note="similar to TALER14"
     CDS             3570..3590
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    3639..3660
                     /label=FF4
                     /note="FF4"
     misc_feature    3661..3682
                     /label=FF4
                     /note="FF4"
     misc_feature    3683..3704
                     /label=FF4
                     /note="FF4"
     misc_feature    3711..3732
                     /label=FF4
                     /note="FF4"
     protein_bind    complement(3753..3777)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3932..3987
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4348..4364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4372..4388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4396..4426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4441..4462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4635..5471
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(5501..5519)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5540..5556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5564..5580)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5588..5618)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5633..5654)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5942..6530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6704..7561)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7562..7666)
                     /label=AmpR promoter
     rep_origin      complement(7692..8147)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8289..8305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8315..8333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8348..9151
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9230..9255
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat tcgcccttca ggtccgaggt tctagacgag
       61 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
      121 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      181 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
      241 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
      301 ataagcagag ctcgtttagt gaaccgtcag atcgcaaagg gcgaattcga cccaagtttg
      361 tacaaaaaag caggctgatt accggagaat tcgtgagcaa gggcgccacc atgaccggtt
      421 tgtcgaccat tcgtccgcgc aggccaagtc ctgcccgcga gcttctgccc ggaccccaac
      481 cggatagggt tcagccgact gcagatcgtg gggtgtctgc gcctgctggc agccctctgg
      541 atggcttgcc cgctcggcgg acggtgtccc ggacccggct gccatctccc cctgcgccct
      601 cacctgcgtt ctcggcgggc agcttcagcg atctgctccg tccgttcgat ccgtcgcttc
      661 ttgatacatc gcttcttgat tcgatgcctg ccgtcggcac gccgcataca gcggctgccc
      721 cagcagagtg ggatgaggcg caatcggctc tgcgtgcagc cgatgacccg ccacccaccg
      781 tgcgtgtcgc tgtcactgcc gcgcggccgc cgcgcgccaa gccggccccg cgacggcgtg
      841 ctgcgcaacc ctccgacgct tcgccggccg cgcaggtgga tctacgcacg ctcggctaca
      901 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
      961 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
     1021 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
     1081 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
     1141 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
     1201 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
     1261 cactgacggg tgcccccctg aacctgaccc cggaccaagt ggtggctatc gccagcaaca
     1321 ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     1381 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     1441 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc
     1501 aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc
     1561 tgttgccggt gctgtgccag gaccatggcc tgactccgga ccaagtggtg gctatcgcca
     1621 gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     1681 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     1741 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     1801 cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc
     1861 agcggctgtt gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta
     1921 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     1981 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     2041 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     2101 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     2161 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg
     2221 tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2281 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca
     2341 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     2401 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     2461 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     2521 aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc
     2581 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     2641 gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     2701 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     2761 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     2821 cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaagcattg
     2881 tggcccagct gagccggcct gatccggcgt tggccgcgtt gaccaacgac cacctcgtcg
     2941 ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga ttgccgcacg
     3001 cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc catcgcgttg
     3061 ccgactacgc gcaagtggtt cgcgtgctgg agtttttcca gtgccactcc cacccagcgt
     3121 acgcatttga tgaggccatg acgcagttcg ggatgagcag gaacgggttg gtacagctct
     3181 ttcgcagagt gggcgtcacc gaactcgaag cccgcggtgg aacgctcccc ccagcctcgc
     3241 agcgttggga ccgtatcctc caggcatcag ggatgaaaag ggccaaaccg tcccctactt
     3301 cagctcaaac accggatcag gcgtctttgc atgcattcgc cgattcgctg gagcgtgacc
     3361 ttgatgcgcc cagcccaatg cacgagggag atcagacgcg ggcaagcagc cgtaaacggt
     3421 cccgatcgga tcgtgctgtc accggcccct ccgcacagca ggctgtcgag gtgcgcgttc
     3481 ccgaacagcg cgatgcgctg catttgcccc tcagctggag ggtaaaacgc ccgcgtacca
     3541 ggatctgggg cggcctcccg gatcccagcc ccaagaagaa gagaaaggtg gaggccagcg
     3601 gtggcggctc aaagcttact agttaagtcg acggtacccc gcttgaagtc tttaattaaa
     3661 ccgcttgaag tctttaatta aaccgcttga agtctttaat taaacccggg ccgcttgaag
     3721 tctttaatta aagtctagat aactgatcta ccacccagct ttcttgtaca aagtggtacg
     3781 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3841 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3901 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3961 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     4021 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     4081 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     4141 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     4201 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     4261 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     4321 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4501 cctgtcgtgc cagcggatcg acagtactaa gcttggtgcg tttttatgct tgtagtattg
     4561 tataatgttt ttaagatcct taattaatag ggataacagg gtaatctcga ggtttaaacg
     4621 tcgacgcggc cgctactagg gacaggattg gtgacagaaa agccccatcc ttaggcctcc
     4681 tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca gattccttat
     4741 ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc tctaaggttt
     4801 gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg tgggagggaa
     4861 gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc tctcccagaa
     4921 cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc tgcagcttcc
     4981 ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag ttggtcctga
     5041 gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct ccgtgcgtca
     5101 gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg tggtgaggag
     5161 gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt gttcaccagg
     5221 tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa gagtccccag
     5281 tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa ggccctgctc
     5341 tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct tccctgtccc
     5401 ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac aggggttcct
     5461 ggctctgctc tccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
     5521 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5581 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5641 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5701 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5761 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5821 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5881 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5941 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6001 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6061 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6121 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6181 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6241 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6301 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6361 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6421 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6481 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6541 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6601 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6661 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6721 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6781 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6841 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6901 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6961 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7021 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7081 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7141 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7201 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7261 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7321 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7381 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7441 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7501 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7561 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7621 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7681 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     7741 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7801 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     7861 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     7921 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     7981 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     8041 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     8101 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     8161 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     8221 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     8281 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     8341 gggtacctgc tttctctgac cagcattctc tcccctgggc ctgtgccgct ttctgtctgc
     8401 agcttgtggc ctgggtcacc tctacggctg gcccagatcc ttccctgccg cctccttcag
     8461 gttccgtctt cctccactcc ctcttcccct tgctctctgc tgtgttgctg cccaaggatg
     8521 ctctttccgg agcacttcct tctcggcgct gcaccacgtg atgtcctctg agcggatcct
     8581 ccccgtgtct gggtcctctc cgggcatctc tcctccctca cccaacccca tgccgtcttc
     8641 actcgctggg ttcccttttc cttctccttc tggggcctgt gccatctctc gtttcttagg
     8701 atggccttct ccgacggatg tctcccttgc gtcccgcctc cccttcttgt aggcctgcat
     8761 catcaccgtt tttctggaca accccaaagt accccgtctc cctggcttta gccacctctc
     8821 catcctcttg ctttctttgc ctggacaccc cgttctcctg tggattcggg tcacctctca
     8881 ctcctttcat ttgggcagct cccctacccc ccttacctct ctagtctgtg ctagctcttc
     8941 cagccccctg tcatggcatc ttccaggggt ccgagagctc agctagtctt cttcctccaa
     9001 cccgggcccc tatgtccact tcaggacagc atgtttgctg cctccaggga tcctgtgtcc
     9061 ccgagctggg accaccttat attcccaggg ccggttaatg tggctctggt tctgggtact
     9121 tttatctgtc ccctccaccc cacagtgggg caagcttatt accctgttat ccctaaccgg
     9181 tttaccgagc tcttattggt tttcaaactt cattgactgt gccaagcttc tgacctcttc
     9241 tcttcctccc acagggcctc gagacaattg attaacatcg atacggtacc gagttggcgc
     9301 gcctgggagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     9361 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     9421 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     9481 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     9541 gaaaaagctt taggctgaaa gagagattta gaatgacagg cgcgcctggc catacatcga
     9601 tacggtaccg agttggcgcg cctgggagct cacggggaca gccccccccc aaagccccca
     9661 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     9721 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     9781 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     9841 acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacaggc
     9901 gcgcctggtg gcgaacgat
//
