LOCUS       40924_44013       11185 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRE-EYFP-2A-TALER9-4xTarget_FF5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 11185)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        4242..4556
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4629..4653
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4676..5392
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5399..5452
                     /label=2A
                     /note="2A"
     CDS             5399..5452
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5468..9007
                     /label=similar to TALER9
                     /note="similar to TALER9"
     CDS             9017..9037
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    9097..9118
                     /label=FF5
                     /note="FF5"
     misc_feature    9119..9140
                     /label=FF5
                     /note="FF5"
     misc_feature    9141..9162
                     /label=FF5
                     /note="FF5"
     misc_feature    9163..9184
                     /label=FF5
                     /note="FF5"
     protein_bind    complement(9218..9242)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9397..9452
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9813..9829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9837..9853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9861..9891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9906..9927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    10100..10936
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(10966..10984)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11005..11021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11029..11045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11053..11083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11098..11119)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
       61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac
     2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc
     2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc
     2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct
     2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc
     2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc
     2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg
     3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca
     3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc
     3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct
     3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc
     3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact
     3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat
     3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc
     3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tgcgttttta tgcttgtagt
     3481 attgtataat gtttttaaga tccaagcttc tgacctcttc tcttcctccc acagggcctc
     3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag
     4201 tgatatcaac tttgtataga aaagttggct ccgaatttct cgagtttact ccctatcagt
     4261 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac
     4321 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     4381 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga
     4441 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc
     4501 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4561 gatttcgagc tcggtacccg gggatcctct agtcagctga cgcgtgctag tggcgcgccg
     4621 aattcgaccc aagtttgtac aaaaaagcag gctgattacc ggagaattcg ccaccatggt
     4681 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4741 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4801 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4861 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4921 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4981 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5041 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5101 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5161 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5221 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5281 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5341 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agcaattgga
     5401 gggccgcggc agcctgctga cctgcggcga cgtggaggaa aaccccggcc ccaccggttt
     5461 gtcgaccatt cgtccgcgca ggccaagtcc tgcccgcgag cttctgcccg gaccccaacc
     5521 ggatagggtt cagccgactg cagatcgtgg ggtgtctgcg cctgctggca gccctctgga
     5581 tggcttgccc gctcggcgga cggtgtcccg gacccggctg ccatctcccc ctgcgccctc
     5641 acctgcgttc tcggcgggca gcttcagcga tctgctccgt ccgttcgatc cgtcgcttct
     5701 tgatacatcg cttcttgatt cgatgcctgc cgtcggcacg ccgcatacag cggctgcccc
     5761 agcagagtgg gatgaggcgc aatcggctct gcgtgcagcc gatgacccgc cacccaccgt
     5821 gcgtgtcgct gtcactgccg cgcggccgcc gcgcgccaag ccggccccgc gacggcgtgc
     5881 tgcgcaaccc tccgacgctt cgccggccgc gcaggtggat ctacgcacgc tcggctacag
     5941 tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga
     6001 ggcactggtg ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc
     6061 agcgttaggg accgtcgctg tcacgtatca gcacataatc acggcgttgc cagaggcgac
     6121 acacgaagac atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt
     6181 gctcacggat gcgggggagt tgagaggtcc gccgttacag ttggacacag gccaacttgt
     6241 gaagattgca aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcat cgcgcaatgc
     6301 actgacgggt gcccccctga acctgacccc ggaccaagtg gtggctatcg ccagcaacat
     6361 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     6421 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     6481 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     6541 agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct
     6601 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     6661 caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     6721 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca
     6781 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     6841 ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca
     6901 gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat
     6961 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     7021 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     7081 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     7141 gaccccggac caagtggtgg ctatcgccag caacaatggc ggcaagcaag cgctcgaaac
     7201 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     7261 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     7321 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     7381 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     7441 tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct
     7501 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ctccggacca
     7561 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     7621 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     7681 caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     7741 ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     7801 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
     7861 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
     7921 gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat
     7981 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     8041 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acattggcgg
     8101 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     8161 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac
     8221 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     8281 ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa agcattgtgg cccagctgag
     8341 ccggcctgat ccggcgttgg ccgcgttgac caacgaccac ctcgtcgcct tggcctgcct
     8401 cggcggacgt cctgccatgg atgcagtgaa aaagggattg ccgcacgcgc cggaattgat
     8461 cagaagagtc aatcgccgta ttggcgaacg cacgtcccat cgcgttgccg actacgcgca
     8521 agtggttcgc gtgctggagt ttttccagtg ccactcccac ccagcgtacg catttgatga
     8581 ggccatgacg cagttcggga tgagcaggaa cgggttggta cagctctttc gcagagtggg
     8641 cgtcaccgaa ctcgaagccc gcggtggaac gctcccccca gcctcgcagc gttgggaccg
     8701 tatcctccag gcatcaggga tgaaaagggc caaaccgtcc cctacttcag ctcaaacacc
     8761 ggatcaggcg tctttgcatg cattcgccga ttcgctggag cgtgaccttg atgcgcccag
     8821 cccaatgcac gagggagatc agacgcgggc aagcagccgt aaacggtccc gatcggatcg
     8881 tgctgtcacc ggcccctccg cacagcaggc tgtcgaggtg cgcgttcccg aacagcgcga
     8941 tgcgctgcat ttgcccctca gctggagggt aaaacgcccg cgtaccagga tctggggcgg
     9001 cctcccggat cccagcccca agaagaagag aaaggtggag gccagcggtg gcggctcaaa
     9061 gcttggtggc ggctcaacta gttaagcggc cgcaaaaagc actctgattt gacaattaaa
     9121 gcactctgat ttgacaatta aagcactctg atttgacaat taaagcactc tgatttgaca
     9181 attaaagctt cgaattctgc atctagataa ctgatctacc cagctttctt gtacaaagtg
     9241 gtacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     9301 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     9361 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     9421 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     9481 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     9541 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     9601 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     9661 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     9721 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     9781 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9841 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9901 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9961 ggaaacctgt cgtgccagcg gatcgacagt actaagcttg gtctaatacc caatctctcg
    10021 tcttatccag atgttttata cgccttaatt aatagggata acagggtaat ctcgaggttt
    10081 aaacgtcgac gcggccgcta ctagggacag gattggtgac agaaaagccc catccttagg
    10141 cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc
    10201 cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa
    10261 ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga
    10321 gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc
    10381 cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag
    10441 cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt
    10501 cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg
    10561 cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg
    10621 aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca
    10681 ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc
    10741 cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc
    10801 tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct
    10861 gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg
    10921 ttcctggctc tgctctccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
    10981 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11161 gtcgtgccag ctgcattaat gaatc
//
