LOCUS       pTRE-Dual1.        3199 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing two genes with the Tet-On(R) Advanced or 
            Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Dual1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    323..366
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     misc_feature    368..954
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    955..1005
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     polyA_signal    1113..1194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1376..1964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2138..2995)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2996..3100)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattcct gcagcccggg ttaattaaca gctgacgcgt
      361 gctagcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      421 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      481 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      541 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      601 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      661 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      721 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      781 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      841 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      901 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccgcggcc
      961 gctagatctg gatccatcga tgtcgacgat atccatatgt ctagaggatc ataatcagcc
     1021 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1081 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1141 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcctcgag
     1201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     2761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3121 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3181 cgaggccctt tcgtcttca
//
