LOCUS       pTRE-Cycle3.        3872 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing ZsGreen1 while cycling the level of a second 
            protein by alternating expression and rapid degradation.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Cycle3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             352..675
                     /label=DD
                     /note="destabilization domain that can be stabilized by
                     Shield1 in the ProteoTuner(TM) system"
     misc_feature    676..737
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    851..932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1114..1702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1876..2733)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2734..2838)
                     /label=AmpR promoter
     polyA_signal    complement(2871..2952)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3094..3786)
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     promoter        3803..3872
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagatttcga gctcggtacc cggggatcac catgggagtg
      361 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgt
      421 gtggtgcact acaccgggat gcttgaagat ggaaagaaag tcgattcctc ccgggacaga
      481 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
      541 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
      601 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
      661 cttctaaaac cggaaggatc ctctagtcag ctgacgcgtg ctagcgcggc cgcatcgata
      721 agcttgtcga cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt
      781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      901 aaatttcaca aataaagcat ttttttcact gccccgagct tcctcgctca ctgactcgct
      961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2461 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     2521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg gcagtgaaaa
     2881 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2941 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3001 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     3061 ctagactgca gcctcaggag atctgggccc tcagggcaag gcggagccgg aggcgatggc
     3121 gtgctcggtc aggtgccact tctggttctt ggcgtcgctg cggtcctcgc gggtcagctt
     3181 gtgctggatg aagtgccagt cgggcatctt gcggggcacg gacttggcct tgtacacggt
     3241 gtcgaactgg cagcgcaagc ggccaccgtc cttcagcagc aggtacatgc tcacgtcgcc
     3301 cttcaagatg ccctgcttgg gcacggggat gatcttctcg caggagggct cccagttgtc
     3361 ggtcatcttc ttcatcacgg ggccgtcggc ggggaagttc acgccgtaga acttggactc
     3421 gtggtacatg cagttctcct ccacgctcac ggtgatgtcg gcgttgcaga tgcacacggc
     3481 gccgtcctcg aacaggaagg agcggtccca ggtgtagccg gcggggcagg agttcttgaa
     3541 gtagtcgacg atgtcctggg ggtactcggt gaacacgcgg ttgccgtaca tgaaggcggc
     3601 ggacaagatg tcctcggcga agggcaaggg gccgccctcc accacgcaca ggttgatggc
     3661 ctgcttgccc ttgaaggggt agccgatgcc ctcgccggtg atcacgaact tgtggccgtc
     3721 cacgcagccc tccatgcggt acttcatggt catctccttg gtcaggccgt gcttggactg
     3781 ggccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct ctgcttatat
     3841 aggcctccca ccgtacacgc cacctcgaca ta
//
