LOCUS       40924_43843        7404 bp DNA     circular SYN 18-DEC-2018
DEFINITION  AAV expression vector pTR-UF50-BC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Marsic D, Govindasamy L, Currlin S, Markusic DM, Tseng YS, Herzog 
            RW, Agbandje-McKenna M, Zolotukhin S.
  TITLE     Vector design Tour de Force: integrating combinatorial and rational 
            approaches to derive novel adeno-associated virus variants
  JOURNAL   Mol. Ther. 22 (11), 1900-1909 (2014)
  PUBMED    25048217
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Zolotukhin S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2013) Pediatrics, University of Florida, 2033 
            Mowry Rd, CGRC 235, Gainesville, FL 32610, USA
REFERENCE   3  (bases 1 to 7404)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Ther."; date: "2014"; volume: "22"; issue: "11"; pages: "1900-1909"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2013) Pediatrics, University of Florida, 2033 Mowry Rd, CGRC
            235, Gainesville, FL 32610, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..151
                     /rpt_family="AAV2 ITR"
     enhancer        345..724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1003
                     /label=chicken beta-actin promoter
     intron          1004..2022
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2084..3733
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3734..3745
                     /label=Region: Furin cleavage sequence
                     /note="Region: Furin cleavage sequence"
     misc_feature    3746..3817
                     /label=Region: 2A
                     /note="Region: 2A"
     CDS             3752..3817
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3818..4525
                     /label=mApple
                     /note="photostable monomeric derivative of DsRed (Shaner et
                     al., 2008)"
     misc_feature    4535..4540
                     /label=Barcode
                     /note="Barcode"
     polyA_signal    4559..4680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4729..4874
                     /rpt_family="AAV2 ITR"
     rep_origin      complement(5116..5704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5878..6735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6736..6840)
                     /label=AmpR promoter
     rep_origin      6867..7295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 agatctttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
       61 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag
      121 ggagtggcca actccatcac taggggttcc tggaggggtg gagtcgtgac gtgaattacg
      181 tcatagggtt agggaggtcc tggatcttca atattggcca ttagccatat tattcattgg
      241 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
      301 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
      361 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      421 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      481 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      541 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      601 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      661 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      721 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      901 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      961 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct
     1021 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     1081 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     1141 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     1201 gggagggccc tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
     1261 gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     1321 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1381 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1441 cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt
     1501 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1561 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1621 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1681 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1741 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1801 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1861 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1921 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1981 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     2041 gttattgtgc tgtctcatca ttttggcaaa gaattccgcc accatggagg atgccaagaa
     2101 tattaagaaa ggccctgccc cattctaccc tctggaagat ggcactgctg gtgagcaact
     2161 gcacaaggcc atgaagaggt atgccctggt ccctggcacc attgccttca ctgatgctca
     2221 cattgaggtg gacatcacct atgctgaata ctttgagatg tctgtgaggc tggcagaagc
     2281 catgaaaaga tatggactga acaccaacca caggattgtg gtgtgctctg agaactctct
     2341 ccagttcttc atgcctgtgt taggagccct gttcattgga gtggctgtgg cccctgccaa
     2401 tgacatctac aatgagagag agctcctgaa cagcatgggc atcagccagc caactgtggt
     2461 ctttgtgagc aagaagggcc tgcaaaagat cctgaatgtg cagaagaagc tgcccatcat
     2521 ccagaagatc atcatcatgg acagcaagac tgactaccag ggcttccaga gcatgtatac
     2581 ctttgtgacc agccacttac cccctggctt caatgagtat gactttgtgc ctgagagctt
     2641 tgacagggac aagaccattg ctctgattat gaacagctct ggctccactg gactgcccaa
     2701 aggtgtggct ctgccccaca gaactgcttg tgtgagattc agccatgcca gagaccccat
     2761 ctttggcaac cagatcatcc ctgacactgc catcctgtct gtggttccat tccatcatgg
     2821 ctttggcatg ttcacaacac tggggtacct gatctgtggc ttcagagtgg tgctgatgta
     2881 taggtttgag gaggagctgt ttctgaggag cctacaagac tacaagatcc agtctgccct
     2941 gctggtgccc actctgttca gcttctttgc caagagcacc ctcattgaca agtatgacct
     3001 gagcaacctg catgagattg cctctggagg agcacccctg agcaaggagg tgggtgaggc
     3061 tgtggcaaag aggttccatc tcccaggaat cagacagggc tatggcctga ctgagaccac
     3121 ctctgccatc ctcatcaccc ctgaaggaga tgacaagcct ggtgctgtgg gcaaggtggt
     3181 tccctttttt gaggccaagg tggtggacct ggacactggc aagaccctgg gagtgaacca
     3241 gaggggtgag ctgtgtgtga ggggtcccat gatcatgtct ggctatgtga acaaccctga
     3301 ggccaccaat gccctgattg acaaggatgg ctggctgcac tctggtgaca ttgcctactg
     3361 ggatgaggat gagcactttt tcattgtgga caggctgaag agcctcatca agtacaaagg
     3421 ctaccaagtg gcacctgctg agctagagag catcctgctc cagcacccca acatctttga
     3481 tgctggtgtg gctggcctgc ctgatgatga tgctggagag ctgcctgctg ctgttgtggt
     3541 tctggagcat ggaaagacca tgactgagaa ggagattgtg gactatgtgg ccagtcaggt
     3601 gaccactgcc aagaagctga ggggaggtgt ggtgtttgtg gatgaggtgc caaagggtct
     3661 gactggcaag ctggatgcca gaaagatcag agagatcctg atcaaggcca agaagggtgg
     3721 caaaatcgcc gtccgcgcga aacgcgcgcc ggtgaaacag accctgaact ttgatctgct
     3781 gaaactggcg ggcgatgtgg aaagcaaccc gggcccgatg gtgagcaagg gcgaggagaa
     3841 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3901 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac
     3961 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4021 tcagttcatg tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt
     4081 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg
     4141 cattattcac gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa
     4201 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4261 ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa
     4321 gaggctgaag ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc
     4381 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc
     4441 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4501 cggcggcatg gacgagctgt acaagtaaat cgatnnnnnn ctcgagccgc ggactagtaa
     4561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4681 tcatgtctta cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag
     4741 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4801 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagag
     4861 agggagtggc caaagatctc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5281 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     5341 gtaggtcgtt cgctccaagc tggctgtgtt gcacgaaccc cccgttcagc ccgaccgctg
     5401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5521 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5641 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6841 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     6901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7021 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7081 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7201 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7261 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa caaaatatta
     7321 acgtttacaa tttcccatta gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7381 cgggcctctt cgctattacg ccag
//
