LOCUS       V015489                 5494 bp    DNA     circular SYN 26-JUN-2019
DEFINITION  Exported.
ACCESSION   V015489
VERSION     V015489
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   O'Connor DM, Lutomski C, Jarrold MF, Boulis NM, Donsante A.
  TITLE     Lot-to-lot Variation in Adeno-associated Virus Serotype 9 (AAV9)
            Preparations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   O'Connor DM, Lutomski C, Jarrold MF, Boulis NM, Donsante A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2018) Neurosurgery, Emory University, 101 Woodruff
            Circle, Room 6339, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 5494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-NOV-2018) Neurosurgery, Emory University, 101 Woodruff Circle,
            Room 6339, Atlanta, GA 30322, USA"
FEATURES             Location/Qualifiers
     source          1..5494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    44..251
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   264..404
     misc_feature    complement(426..920)
                     /label="similar to pGFIB-1 in INSD accession LC121522.1"
                     /note="similar to pGFIB-1 in INSD accession LC121522.1"
     promoter        1202..1306
                     /label="AmpR promoter"
     CDS             1307..2164
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2338..2926
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      3533..4046
                     /direction=RIGHT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   4090..4195
                     /label="mutant"
                     /note="mutant"
     regulatory      4242..4521
                     /label="CMV enhancer"
                     /note="CMV enhancer"
                     /regulatory_class="enhancer"
     regulatory      4528..4797
                     /label="derived from chicken B-actin"
                     /note="derived from chicken B-actin"
                     /regulatory_class="promoter"
     intron          4863..4959
                     /label="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
     CDS             5031..5489
                     /gene="IGF1"
                     /label="Insulin-like growth factor I"
                     /note="Insulin-like growth factor I from Ailuropoda
                     melanoleuca. Accession#: Q6JLX1"
ORIGIN
        1 agcttatcga taccgtcgac tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
       61 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      121 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
      181 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
      241 catgctgggg agagatcgat ctgaggaacc cctagtgatg gagttggcca ctccctctct
      301 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
      361 ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga gtggcccccc cccccccccc
      421 cccggcgatt ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagag
      481 acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc
      541 atattgatgg tgatttgact gtctccggcc tttctcaccc gtttgaatct ttacctacac
      601 attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg
      661 aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt
      721 tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg
      781 atttattgga tgttggaatc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      841 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      901 gccccgacac ccgccaacac tatggtgcac tctcagtaca atctgctctg atgccgcata
      961 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     1021 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1081 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     1141 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1201 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1261 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1321 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1381 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1441 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1501 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1561 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1621 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1681 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1741 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1801 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1861 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1921 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1981 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2041 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2101 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2161 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2221 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2461 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2761 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2821 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2881 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2941 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3001 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3061 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3121 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcgta atagcgaaga
     3181 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgattccg
     3241 ttgcaatggc tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt
     3301 cttctactca ggcaagtgat gttattacta atcaaagaag tattgcgaca acggttaatt
     3361 tgcgtgatgg acagactctt ttactcggtg gcctcactga ttataaaaac acttctcagg
     3421 attctggcgt accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct
     3481 ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc
     3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3961 tttaacaaaa tattaacgct tacaatttaa atatttgctt atacaatctt cctgtttttg
     4021 gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg
     4081 ttcatcgccc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg
     4141 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggaattc
     4201 acgcgtggat ctgaattcaa ttcacgcgtg gtacctctgg tcgttacata acttacggta
     4261 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4321 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4381 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4441 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4501 cctacttggc agtacatcta ctcgaggcca cgttctgctt cactctcccc atctcccccc
     4561 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     4621 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     4681 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     4741 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagcgg
     4801 gatcagccac cgcggtggcg gcctagagtc gacgaggaac tgaaaaacca gaaagttaac
     4861 tggtaagttt agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc
     4921 aaagaactgc tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt
     4981 ctgctctaaa agctgcggaa ttgtacccgc ggccgatcca ccggtccacc atgggaaaaa
     5041 tcagcagtct tccaacccaa ttatttaagt gctgcttttg tgacttcttg aaggtgaaga
     5101 tgcacaccat gtcctcctcg catctcttct acctggcgct gtgcctgctc accttcacca
     5161 gctctgccac ggctggaccg gagacgctct gcggggctga gctggtggat gctcttcagt
     5221 tcgtgtgtgg agacaggggc ttttatttca acaagcccac agggtatggc tccagcagtc
     5281 ggagggcgcc tcagacaggc atcgtggatg agtgctgctt ccggagctgt gatctaagga
     5341 ggctggagat gtattgcgca cccctcaagc ctgccaagtc agctcgctct gtccgtgccc
     5401 agcgccacac cgacatgccc aagacccaga aggaagtaca tttgaagaac gcaagtagag
     5461 ggagtgcagg aaacaagaac tacaggatgt gaga
//
