LOCUS       40924_43838        4710 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTPTK3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 4710)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Extremophiles (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux, 
            Paris 75015, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1336..1809
                     /codon_start=1
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /label=pyruvoyl-dependent arginine decarboxylase
                     /protein_id="AVP12442.1"
                     /translation="MSWTTPKRAFIGAATAEGGTKLNAFDNALLKLGIGNVNLVKLSSV
                     IPAHIEWMEKVHDVPIGMLLPTVYAHIESDEPGMTISAALGVGISKNNEGGLIYEYSGY
                     CTKEEAEEMVRKMVEEGFRQRGWELGEFKVASAEITVKDKPAAAIAVVVMFPY"
     CDS             complement(1837..1971)
                     /codon_start=1
                     /product="transmembrane domain protein"
                     /label=transmembrane domain protein
                     /protein_id="AVP12441.1"
                     /translation="MSKKLDVKVKVNIYVNVHIDIKVAISIAVTIAVIALLKVLLNIR"
     CDS             2074..2223
                     /codon_start=1
                     /product="SpoVT/AbrB-like transcriptional regulator"
                     /label=SpoVT/AbrB-like transcriptional regulator
                     /note="swapped-hairpin fold CDS"
                     /protein_id="AVP12440.1"
                     /translation="MQDVRKMFRSGSSYVVAFPPSVIQKTKFRHQRTVRVRIYDDKIVI
                     VPLW"
     CDS             2886..3644
                     /codon_start=1
                     /product="RepTP2"
                     /label=RepTP2
                     /protein_id="AVP12439.1"
                     /translation="MTLMCLWFHTRTRFTSRAMLVQKLERYDSLFKVASARHTDAVFLT
                     LTTDPSRFSNLYEANRQFSHSFNRFMSRLRGYFARRGQHLEYIAVYEFTKSGLLHAHVI
                     IFGVRYLLPVRVISRWWSEAGQGRVVYIYRLRNVDGRWVWARRRPRDVRAGEGAEDYLK
                     KYLRKALRLASTLDTSDVKKVLPLALYWAFNKRFFTYSRSLLPSSRRTAEAVVSEYLWV
                     GTYRLEDLPDWVFWLPHRVYSPPSRGPPPT"
     CDS             complement(3693..4349)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4350..4452)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
       61 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
      121 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
      181 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
      241 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
      301 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
      421 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
      481 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
      541 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
      601 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
      661 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
      721 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
      781 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
      841 tatttgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct ggggccctag
     1081 gcctgcggcc gcatgctcgc gacatgtcga cgatatccat atcggatcct cgttctctac
     1141 tcaatggcat cgagaatgat gttttagcgc gttttccccc catgggcctt ctatgctaca
     1201 aaaacacccc caaatcgcct cttttagata tagcgaaacg tttttaagct ggtaaagaag
     1261 tcgcgtttga ttaaacagtc tgctcatcta ataacaggag gagagtatac ctaaagagaa
     1321 aaggaggagg ttgagatgag ctggacaacc ccaaagagag cttttatagg tgccgcaacc
     1381 gctgagggag ggactaagct gaacgccttt gacaacgcac tcctcaagct gggcatagga
     1441 aacgttaacc tggtcaagct aagcagcgtt attcccgcac atatcgagtg gatggagaag
     1501 gtacacgacg ttccgatagg aatgctcctg ccgacagttt acgcccacat cgagagcgac
     1561 gagccgggga tgacgataag cgccgcactg ggcgtcggga taagcaagaa caacgaaggc
     1621 ggtctgatct acgagtattc gggctactgc accaaggaag aagccgagga aatggtcagg
     1681 aagatggtcg aagaaggctt caggcagagg ggctgggagc tcggtgagtt caaggttgca
     1741 agcgccgaga taaccgtcaa ggacaagcca gccgccgcaa tagcggtcgt cgtcatgttc
     1801 ccctactgag ggtttgagaa gagaagaaaa gggttctcag cgaatgttga ggagaacttt
     1861 gagcagtgcg atgactgcga tagtgaccgc gatgctgatg gcgaccttga tgtcgatgtg
     1921 gacgttcacg tagatgttga ccttgacctt gacgtcgagt ttcttcgaca tcacttactt
     1981 gtcacggggt agtatatatg cttgagccgt ccgtcgctat aaatgccttc gagggtacta
     2041 ttcttgtggt gagcgccggt gtcgcgtctc ataatgcaag acgtgaggaa gatgttccgc
     2101 tctgggagta gctacgttgt cgcgttcccg ccgtcggtca tccagaagac gaagttccga
     2161 caccaaagga ccgtgcgcgt tcgcatctat gatgacaaga ttgtcattgt gcccctctgg
     2221 taggggttga gcaaggctta tatttttcct ccgtcaagag tatgaagaag tgggcgggtg
     2281 cccccacacc cgcccgcgat tccccccgac acaactgcaa aggaggtact gccatatgtg
     2341 tgtagaccat aggtctgaaa aacttgacgg tttgaagttg gacggtcggt tacgccacgt
     2401 gcttgaggtt gtcgttgagc ttgccggcga gtatggtggt agtgtcaatt tgccgtttct
     2461 tgccagagtg gtctcagcat caactggctc cggtagcaag cggaagactg agtatgccaa
     2521 gcttcgtcgg gatatcaaca aactgaggga tatgggtctt gtttcggttg aaaaactcga
     2581 tggcttcctc tgggtgtctc ctactcccaa ggctctttac ttgattgaac ataggagaaa
     2641 ttcaaatcgt gtccgtaggt cgatgcgtcc ggaaaaagcg cgtgcaatac agcttcttcg
     2701 gtgtatagat atcccgggct attttgtttg gtcgaaagat ggcaagaaca aggttgctga
     2761 gggtgtggtc aacgggctca aggggtacat cgactatgct gaaagtatcc gtgtcgttct
     2821 tgttcgccgt gaacttgtcg ttggtggtgc gtcaactctc gatgaactgg ggcgtcttgc
     2881 agtagatgac tctgatgtgc ttgtggttcc atacccgtac gcggttcacg tcacgggcga
     2941 tgcttgtcca gaagcttgag cgctatgatt ctctcttcaa agtggcgtcg gctcggcaca
     3001 ctgatgcggt gttcttgact ctcacaactg acccatcacg tttttcgaat ctctatgagg
     3061 cgaatcgtca attttctcac tcgttcaacc gttttatgtc gcgcttgcgt ggctactttg
     3121 cacgccgggg acaacatctc gagtatattg cggtgtatga gttcacgaag tcgggattgc
     3181 tccacgcgca cgtcatcatt ttcggcgtcc gctacctctt gccggttcga gtgatatccc
     3241 ggtggtggtc ggaggctggt caagggcgtg ttgtctacat ctatcgtctt cgtaatgttg
     3301 atggtcggtg ggtttgggcg cgccgtcgtc cgcgcgacgt gcgcgcgggc gagggcgcgg
     3361 aggattatct gaaaaagtac cttcgaaagg cattgcgcct tgcgagtact cttgacactt
     3421 ccgacgtcaa gaaagtcctc cccctcgctc tctattgggc attcaacaag cggttcttca
     3481 catactcgcg gtcgttgctt ccctcctcgc gccggacggc agaggcggta gtgtcagagt
     3541 acttgtgggt gggtacctac cgtttggagg accttcccga ctgggttttc tggttgcctc
     3601 accgtgtgta ctctcctccc tctcggggtc cccctccgac gtgacgtagc accaggcgtt
     3661 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     3721 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     3781 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     3841 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     3901 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     3961 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     4021 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     4081 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     4141 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     4201 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     4261 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     4321 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     4381 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     4441 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     4501 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     4561 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     4621 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     4681 gcgtaacggc aaaagcaccg ccggacatca
//
