LOCUS       V002562                 5455 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002562
VERSION     V002562
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
   PUBMED   29497842
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 5455)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5455)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Extremophiles (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux,
            Paris 75015, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             1253..2551
                     /gene="trpE"
                     /label="Anthranilate synthase component 1"
                     /note="Anthranilate synthase component 1 from Thermococcus
                     kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1).
                     Accession#: Q9YGB3"
     CDS             complement(2582..2716)
                     /codon_start=1
                     /product="transmembrane domain protein"
                     /label="transmembrane domain protein"
                     /protein_id="AVP12436.1"
                     /translation="MSKKLDVKVKVNIYVNVHIDIKVAISIAVTIAVIALLKVLLNIR"
     CDS             2819..2968
                     /codon_start=1
                     /product="SpoVT/AbrB-like transcriptional regulator"
                     /label="SpoVT/AbrB-like transcriptional regulator"
                     /note="swapped-hairpin fold CDS"
                     /protein_id="AVP12435.1"
                     /translation="MQDVRKMFRSGSSYVVAFPPSVIQKTKFRHQRTVRVRIYDDKIVI
                     VPLW"
     CDS             3631..4389
                     /codon_start=1
                     /product="RepTP2"
                     /label="RepTP2"
                     /protein_id="AVP12434.1"
                     /translation="MTLMCLWFHTRTRFTSRAMLVQKLERYDSLFKVASARHTDAVFLT
                     LTTDPSRFSNLYEANRQFSHSFNRFMSRLRGYFARRGQHLEYIAVYEFTKSGLLHAHVI
                     IFGVRYLLPVRVISRWWSEAGQGRVVYIYRLRNVDGRWVWARRRPRDVRAGEGAEDYLK
                     KYLRKALRLASTLDTSDVKKVLPLALYWAFNKRFFTYSRSLLPSSRRTAEAVVSEYLWV
                     GTYRLEDLPDWVFWLPHRVYSPPSRGPPPT"
     CDS             complement(4438..5094)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5095..5197)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
       61 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
      121 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
      181 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
      241 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
      301 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
      421 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
      481 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
      541 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
      601 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
      661 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
      721 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
      781 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
      841 tatttgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct ggggccctag
     1081 gcctgcggcc gcatgctcgc gacatgtcga cgatatccat atcggatccg tcgactgccg
     1141 caacgcgcat tttgctcacc cgaaaatttt taaatactaa gggttaattt aatctcgagc
     1201 gtttgagtcc ttctgacggc tcttggagag ggccgttaaa aaggtgatgc atatgcctct
     1261 caaaaagctg aagcccgttg accctttgaa gctctacagc gcccttagag actttgggat
     1321 gccattcatg ctccgctctg ccgagaagga ctccaggaag gccagattca cctacatatc
     1381 ggccgagccg gagttcgtcg tggaggtcgg cgaggggact gagatcgacg gggagcgagt
     1441 ttccgacgag aggaaccccc tcagagctct taaagggctc atgggggaga gggtcgaggg
     1501 caggaggttc atgggtggct tcgttggcta cgtctcctac gattcggtac actccatcat
     1561 cggggggaag atcgaagagc cctcggtctt cggctactac ccctggacct tcatctacga
     1621 ccactctacc ggcgctcttt ccttctttta cctcagagag gctcctttcg accccgaggc
     1681 cttagttgaa agggccagga gggaagagtc acggcttgaa gacggcggtt cggaggtcat
     1741 atccaccgac gcgggcatgg aagagttcgt tgaaatcgtc agggctggga aggagtacat
     1801 ctactcgggg gacgtcttcc aggtggttct gtcgcgcgag tacagggtta gaacggatct
     1861 cgatgccctc gaaatctaca agcggctcgt ggagctcaac ccctccccgt acaccttcat
     1921 cctggagttc gagaagaccg tcgtaggggc ctcacccgaa accatgggtt ccgtcgaggg
     1981 gagaaccttc aagataaacc ccatagcagg aacagcgccg aggggaagga cgggggagga
     2041 agaccgggag ctggaaaagg ccctactctc cgacgagaaa gagcgagctg agcacgtcat
     2101 gctcgttgac cttgctagaa acgacgtcag gagggtttca aagcccggga gcgttaggct
     2161 aacccgcttc ttcgacgtcc tgaagtacag ccacgtccag cacatagaga gcgaggtggt
     2221 cggtgaactc gatgagggga aaaacgcgtt cgacgccatg gaggcggctt ttccggcggg
     2281 aacactaacc ggagccccga agataagggc gatggagatc atagacgagc tggagaggag
     2341 caggagaaag gtctacgggg gagcagtggg ttacttctcc ctcaccgggg acgccgacat
     2401 ggcgatagcg ataaggatgg ccgagatcga gggcaggaaa gcgagcgtta gggccggggc
     2461 aggaatagtg gcggattcag ttccagagaa ggagttcttc gagaccgaga acaaaatgag
     2521 agcagttctg aaggcgctgg gggtgaggga atgagggttt gagaagagaa gaaaagggtt
     2581 ctcagcgaat gttgaggaga actttgagca gtgcgatgac tgcgatagtg accgcgatgc
     2641 tgatggcgac cttgatgtcg atgtggacgt tcacgtagat gttgaccttg accttgacgt
     2701 cgagtttctt cgacatcact tacttgtcac ggggtagtat atatgcttga gccgtccgtc
     2761 gctataaatg ccttcgaggg tactattctt gtggtgagcg ccggtgtcgc gtctcataat
     2821 gcaagacgtg aggaagatgt tccgctctgg gagtagctac gttgtcgcgt tcccgccgtc
     2881 ggtcatccag aagacgaagt tccgacacca aaggaccgtg cgcgttcgca tctatgatga
     2941 caagattgtc attgtgcccc tctggtaggg gttgagcaag gcttatattt ttcctccgtc
     3001 aagagtatga agaagtgggc gggtgccccc acacccgccc gcgattcccc ccgacacaac
     3061 tgcaaaggag gtactgccat atgtgtgtag accataggtc tgaaaaactt gacggtttga
     3121 agttggacgg tcggttacgc cacgtgcttg aggttgtcgt tgagcttgcc ggcgagtatg
     3181 gtggtagtgt caatttgccg tttcttgcca gagtggtctc agcatcaact ggctccggta
     3241 gcaagcggaa gactgagtat gccaagcttc gtcgggatat caacaaactg agggatatgg
     3301 gtcttgtttc ggttgaaaaa ctcgatggct tcctctgggt gtctcctact cccaaggctc
     3361 tttacttgat tgaacatagg agaaattcaa atcgtgtccg taggtcgatg cgtccggaaa
     3421 aagcgcgtgc aatacagctt cttcggtgta tagatatccc gggctatttt gtttggtcga
     3481 aagatggcaa gaacaaggtt gctgagggtg tggtcaacgg gctcaagggg tacatcgact
     3541 atgctgaaag tatccgtgtc gttcttgttc gccgtgaact tgtcgttggt ggtgcgtcaa
     3601 ctctcgatga actggggcgt cttgcagtag atgactctga tgtgcttgtg gttccatacc
     3661 cgtacgcggt tcacgtcacg ggcgatgctt gtccagaagc ttgagcgcta tgattctctc
     3721 ttcaaagtgg cgtcggctcg gcacactgat gcggtgttct tgactctcac aactgaccca
     3781 tcacgttttt cgaatctcta tgaggcgaat cgtcaatttt ctcactcgtt caaccgtttt
     3841 atgtcgcgct tgcgtggcta ctttgcacgc cggggacaac atctcgagta tattgcggtg
     3901 tatgagttca cgaagtcggg attgctccac gcgcacgtca tcattttcgg cgtccgctac
     3961 ctcttgccgg ttcgagtgat atcccggtgg tggtcggagg ctggtcaagg gcgtgttgtc
     4021 tacatctatc gtcttcgtaa tgttgatggt cggtgggttt gggcgcgccg tcgtccgcgc
     4081 gacgtgcgcg cgggcgaggg cgcggaggat tatctgaaaa agtaccttcg aaaggcattg
     4141 cgccttgcga gtactcttga cacttccgac gtcaagaaag tcctccccct cgctctctat
     4201 tgggcattca acaagcggtt cttcacatac tcgcggtcgt tgcttccctc ctcgcgccgg
     4261 acggcagagg cggtagtgtc agagtacttg tgggtgggta cctaccgttt ggaggacctt
     4321 cccgactggg ttttctggtt gcctcaccgt gtgtactctc ctccctctcg gggtccccct
     4381 ccgacgtgac gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     4441 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     4501 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     4561 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     4621 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     4681 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     4741 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     4801 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     4861 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     4921 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     4981 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     5041 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     5101 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     5161 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     5221 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     5281 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     5341 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
     5401 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catca
//
