LOCUS       40924_43828        5489 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTPTK1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 5489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Extremophiles (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux, 
            Paris 75015, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(1130..2356)
                     /codon_start=1
                     /product="HMG-CoA reductase"
                     /label=HMG-CoA reductase
                     /protein_id="AVP12432.1"
                     /translation="MEIEEIIEKVARGEIKFHQVENYVNGDKRLATEIRRRALEKKLGI
                     QLKHIGHYSIDPNEVIGRNIENMIGVVQIPMGIAGPLKINGEYAKGEFYIPLATTEGAL
                     VASVNRGCSALTEAGGVYTTLIDDKMTRAPLLKCPNARRAREVAEWVKNNLDYLQEKAV
                     SKVTRHGKLRGVKPFIVGRNLYLRFEFETGDAMGMNMVTIASEEIMKVIEEEFPDVKYL
                     ALSGNLCVDKKPNALNFILGRGKTIIAEAVVPREIVKKKLKTTPELIAEVNYLKNLVGS
                     AQAGSYGFNAHFANIVGAIFLATGQDEAQITEGAHGITLAEVTEDGDLYISITMPSLEI
                     GTVGGGTRVPPQREALEIMGVAGGGDPPGMNAKKFAEIVAGAVLAGELSLLAAIAAKHL
                     ARAHKMLGR"
     CDS             complement(2616..2750)
                     /codon_start=1
                     /product="transmembrane domain protein"
                     /label=transmembrane domain protein
                     /protein_id="AVP12431.1"
                     /translation="MSKKLDVKVKVNIYVNVHIDIKVAISIAVTIAVIALLKVLLNIR"
     CDS             2853..3002
                     /codon_start=1
                     /product="SpoVT/AbrB-like transcriptional regulator"
                     /label=SpoVT/AbrB-like transcriptional regulator
                     /note="swapped-hairpin fold CDS"
                     /protein_id="AVP12430.1"
                     /translation="MQDVRKMFRSGSSYVVAFPPSVIQKTKFRHQRTVRVRIYDDKIVI
                     VPLW"
     CDS             3115..3738
                     /codon_start=1
                     /product="putative transcriptional regulator"
                     /label=putative transcriptional regulator
                     /note="wHTH fold CDS"
                     /protein_id="AVP12429.1"
                     /translation="MCVDHRSEKLDGLKLDGRLRHVLEVVVELAGEYGGSVNLPFLARV
                     VSASTGSGSKRKTEYAKLRRDINKLRDMGLVSVEKLDGFLWVSPTPKALYLIEHRRNSN
                     RVRRSMRPEKARAIQLLRCIDIPGYFVWSKDGKNKVAEGVVNGLKGYIDYAESIRVVLV
                     RRELVVGGASTLDELGRLAVDDSDVLVVPYPYAVHVTGDACPEA"
     CDS             3665..4423
                     /codon_start=1
                     /product="RepTP2"
                     /label=RepTP2
                     /protein_id="AVP12428.1"
                     /translation="MTLMCLWFHTRTRFTSRAMLVQKLERYDSLFKVASARHTDAVFLT
                     LTTDPSRFSNLYEANRQFSHSFNRFMSRLRGYFARRGQHLEYIAVYEFTKSGLLHAHVI
                     IFGVRYLLPVRVISRWWSEAGQGRVVYIYRLRNVDGRWVWARRRPRDVRAGEGAEDYLK
                     KYLRKALRLASTLDTSDVKKVLPLALYWAFNKRFFTYSRSLLPSSRRTAEAVVSEYLWV
                     GTYRLEDLPDWVFWLPHRVYSPPSRGPPPT"
     CDS             complement(4472..5128)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5129..5231)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
       61 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
      121 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
      181 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
      241 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
      301 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
      421 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
      481 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
      541 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
      601 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
      661 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
      721 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
      781 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
      841 tatttgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct ggggccctag
     1081 gcctgcggcc gcatgctcgc gacatgtcga cgatatccat atcggatcct catctcccaa
     1141 gcattttatg agccctagcc aaatgtttgg cggcaattgc ggccagaaga gagagctcac
     1201 cagccaaaac cgcaccagca actatctccg caaatttctt agcattcatc cctggaggat
     1261 ctcctcctcc agcaaccccc ataatttcta gagcttctct ttgaggaggt actctagtcc
     1321 cacctccaac cgtccctatt tctaggctcg gcattgttat gcttatgtat agatctccat
     1381 cttcagtgac ttcagcgagg gttattccat gtgcaccttc cgtaatttga gcctcatcct
     1441 gacctgtggc taggaatatc gcccctacaa tgttggcaaa gtgagcgtta aaaccgtagg
     1501 aacctgcttg ggccgaaccc actaagttct ttaggtagtt tacttcagcg ataagctctg
     1561 gagtggtctt tagctttttc ttaacaatct ctctaggaac tacagcttca gcaataatgg
     1621 tctttcctct tcccaggatg aagtttaagg cgtttggctt cttatctacg cacagattac
     1681 cagaaagagc caaatatttt acatcgggga attcttcttc tattactttc attatctctt
     1741 cgctcgctat tgtaaccatg ttcattccca tggcatctcc agtttcgaat tcaaatctta
     1801 agtataagtt tctcccaacg ataaaaggct ttacccccct aagctttccg tgacgagtaa
     1861 ccttactaac agctttttct tggaggtaat ccagattatt cttgacccat tcagctactt
     1921 ctctcgccct tctggcgttt ggacatttca atagaggagc tctagtcatc ttgtcatcta
     1981 ttagggttgt gtaaactcca ccagcctctg ttagagcaga acaacctctg tttactgaag
     2041 caaccaaggc cccttcagtt gtggctaggg gaatgtaaaa ctcacctttt gcatattcac
     2101 cattaatctt tagaggaccc gcaataccca tgggtatttg aacgacacct atcatgtttt
     2161 caatgttcct tccaataact tcgtttggat caattgagta gtggccaatg tgctttagct
     2221 gtattccaag ttttttctcc aaggctctcc ttcttatctc agttgcaagc cttttatccc
     2281 cattcacata gttttctact tgatgaaact tgatctctcc cctagcaact ttctctataa
     2341 tctcctctat ttccattcat accacctctg cagtaattta ggtaatatga gaggttaact
     2401 taggcgtttg catatataaa cctttcgata aacaggctgg aaaacagtct gttttgaaca
     2461 aaaagttaaa tagtgaaatt tccaaaagaa caaaggccaa ttctcggaga tttcgccaaa
     2521 aggagcgatg tacatcgagg aatttttata caccaaagga cttttacgtt catcaaagtt
     2581 catctagagg gtttgagaag agaagaaaag ggttctcagc gaatgttgag gagaactttg
     2641 agcagtgcga tgactgcgat agtgaccgcg atgctgatgg cgaccttgat gtcgatgtgg
     2701 acgttcacgt agatgttgac cttgaccttg acgtcgagtt tcttcgacat cacttacttg
     2761 tcacggggta gtatatatgc ttgagccgtc cgtcgctata aatgccttcg agggtactat
     2821 tcttgtggtg agcgccggtg tcgcgtctca taatgcaaga cgtgaggaag atgttccgct
     2881 ctgggagtag ctacgttgtc gcgttcccgc cgtcggtcat ccagaagacg aagttccgac
     2941 accaaaggac cgtgcgcgtt cgcatctatg atgacaagat tgtcattgtg cccctctggt
     3001 aggggttgag caaggcttat atttttcctc cgtcaagagt atgaagaagt gggcgggtgc
     3061 ccccacaccc gcccgcgatt ccccccgaca caactgcaaa ggaggtactg ccatatgtgt
     3121 gtagaccata ggtctgaaaa acttgacggt ttgaagttgg acggtcggtt acgccacgtg
     3181 cttgaggttg tcgttgagct tgccggcgag tatggtggta gtgtcaattt gccgtttctt
     3241 gccagagtgg tctcagcatc aactggctcc ggtagcaagc ggaagactga gtatgccaag
     3301 cttcgtcggg atatcaacaa actgagggat atgggtcttg tttcggttga aaaactcgat
     3361 ggcttcctct gggtgtctcc tactcccaag gctctttact tgattgaaca taggagaaat
     3421 tcaaatcgtg tccgtaggtc gatgcgtccg gaaaaagcgc gtgcaataca gcttcttcgg
     3481 tgtatagata tcccgggcta ttttgtttgg tcgaaagatg gcaagaacaa ggttgctgag
     3541 ggtgtggtca acgggctcaa ggggtacatc gactatgctg aaagtatccg tgtcgttctt
     3601 gttcgccgtg aacttgtcgt tggtggtgcg tcaactctcg atgaactggg gcgtcttgca
     3661 gtagatgact ctgatgtgct tgtggttcca tacccgtacg cggttcacgt cacgggcgat
     3721 gcttgtccag aagcttgagc gctatgattc tctcttcaaa gtggcgtcgg ctcggcacac
     3781 tgatgcggtg ttcttgactc tcacaactga cccatcacgt ttttcgaatc tctatgaggc
     3841 gaatcgtcaa ttttctcact cgttcaaccg ttttatgtcg cgcttgcgtg gctactttgc
     3901 acgccgggga caacatctcg agtatattgc ggtgtatgag ttcacgaagt cgggattgct
     3961 ccacgcgcac gtcatcattt tcggcgtccg ctacctcttg ccggttcgag tgatatcccg
     4021 gtggtggtcg gaggctggtc aagggcgtgt tgtctacatc tatcgtcttc gtaatgttga
     4081 tggtcggtgg gtttgggcgc gccgtcgtcc gcgcgacgtg cgcgcgggcg agggcgcgga
     4141 ggattatctg aaaaagtacc ttcgaaaggc attgcgcctt gcgagtactc ttgacacttc
     4201 cgacgtcaag aaagtcctcc ccctcgctct ctattgggca ttcaacaagc ggttcttcac
     4261 atactcgcgg tcgttgcttc cctcctcgcg ccggacggca gaggcggtag tgtcagagta
     4321 cttgtgggtg ggtacctacc gtttggagga ccttcccgac tgggttttct ggttgcctca
     4381 ccgtgtgtac tctcctccct ctcggggtcc ccctccgacg tgacgtagca ccaggcgttt
     4441 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     4501 gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     4561 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     4621 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     4681 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     4741 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     4801 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     4861 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     4921 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     4981 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     5041 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     5101 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     5161 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     5221 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     5281 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     5341 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     5401 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     5461 cgtaacggca aaagcaccgc cggacatca
//
