LOCUS       Exported                6531 bp DNA     circular SYN 31-AUG-2024
DEFINITION  Cloning vector pTOR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Belbahri L, Torche S, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Belbahri L, Torche S, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 6531)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6531)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6531)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1011..1575
                     /label=5' Bremia lactucae Ham34 promoter
                     /note="5' Bremia lactucae Ham34 promoter"
                     /regulatory_class="promoter"
     primer_bind     1588..1604
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1689..2209
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2217..2800
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             2816..3607
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      3797..4016
                     /label=3' Bremia lactucae Hsp70 terminator
                     /note="3' Bremia lactucae Hsp70 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4343..4361)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4382..4398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4406..4422)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4430..4460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4475..4496)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4784..5372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5546..6403)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6404..6508)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gcccattata cctcgtattc gattaaatga tacaactcat tgccacaccg caagacggcg
      721 tcaccaccat agacagcctt tcaagcaccc ccttgtctat gagctgccac gtcagctgcc
      781 gctcctaata tccacccgcg tccctattcc cgtcatatgg acgtacttga tggtaaaaat
      841 caagggccaa ctgactaccc acggaaaaat cgctatccca tcaaagcagt cgagtttttc
      901 cttttttttt cttatctttt tcaagtcaaa tcaaaatgtc gctaccataa ccattatacc
      961 gccctcaaaa taaagtctat ccctcctatc cactggtcgg ttcctctccc tctgatggac
     1021 aaagggtcgc ctctacgtac ccgcgacaat ctcggctttt ccaatgtcga cagctggcat
     1081 atcgcacgcg aggttaggcg tcttcagccg gcgtaaccac aaacctcgag tcaagtaccc
     1141 tttttcagac cttaatttcc gtgtccattc cctcgcgttt acacgaaggt ttctgagaaa
     1201 taccacatgt cacagagaga gagaagattg gagaaccttc tcaacctgac ccgtgcagag
     1261 cgcttttgcg tcctaccatc cgttaattat agcaccacca ccacatgtct cgccggaact
     1321 ctcttatccg ctctttttga tcgtcttttt tgcgccccat taaaagacat ctcttgcaca
     1381 gggatcgttt ctgattggcc atttcacttc ctcttttaca tccgaaaacc acctgtttgt
     1441 ggggttgtga agccacaacc ggacgctcaa aaagcctcgc ccgactcgcc cacgatcgga
     1501 aggctcattc tccttttcac tctcacgtgc ccaagtccca accgactctt tttcgacctt
     1561 cactctcacc gacaacgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
     1621 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg ggtgcctact
     1681 tcttgtaagc cgaaggtcga gctgtgtgca ctgagtgcga agttgcagat tttgtgtcta
     1741 tgcatttaat acaatttcct tttttccatt ttcgttgttt ttttttgtga aagaacgatt
     1801 gttttcaaaa gaaggccgtt tgtagaacaa ttttttttta tactagcatt tggtattcga
     1861 gtgtgacgaa tcggtgatgg cgctttacaa atgaaatata acgctgaaca agcgcagtga
     1921 cagctttatt ttataacaga gaataaagac caaagtagag tagactgatg gcggcttgaa
     1981 acaaaactac gtgacttcta atgcaaagaa aaccataccc cacgctactt agaattgttt
     2041 ctctttggtt gatgaccgct atagtcgtat ggagtgatcc ctttttgtca atatttcgat
     2101 tcttgctaaa accgtgacta ttttgaggca actgtgtgta cttaacatgg tagaaatttc
     2161 tatgtttaag ccagctgaca atcattcttt atttatcgta aaccaaccac catatgcgtc
     2221 aatgcaattg actgtgtcct cgattatttc tattattaat attatttcca ttaaaaaaga
     2281 aatccagttc gtggcaaaat aagacaaagg tttaaataaa aatttatcgt tattaaactc
     2341 gtaacttacc ttcttcatgg ctcaacccaa tctgtatatc gacttattta atttggttaa
     2401 caaatcggtt ttcgtcgcaa ataggggatg acaactaaaa taccaatttg tgtccttgcg
     2461 acacgtcaac aggtggctgc caatatctca ggttttgagc acacaacgct gtgattggct
     2521 atctgtagat cgcttcgaga actttcggac cgacatatca gaacgcagtc atgtccaatc
     2581 cagctaacga ttggtcgaaa ccgcacttca tcgcttcatt tcaaccaatt cccatctact
     2641 aaaacgagcg tcggaatagt ctcgaacgta accccacgta gtgggtggca gcgcccctcg
     2701 tatctttcaa tcgccactcg agtctcaagt ttgctcactt tgaaattttc catctggact
     2761 acacatcaaa tctaactcaa cttttatcaa ttgaaacaca agggccccgt ttcgcatgat
     2821 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2881 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     2941 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3001 cgagccagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3061 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3121 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3181 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3241 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3301 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     3361 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3421 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3481 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3541 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3601 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     3661 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     3721 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3781 cccccggatc cccggggtcg actagaatac ttagcaattg gttagtttga aaattgtcaa
     3841 agcagagttt tttcgacaat aaaatttatt ttctaaatat ttgatttagc gttgacttaa
     3901 tttgttgatt cggttgtcat cttttgtgaa gatgcccgag agtctagtaa atctcaagcg
     3961 aaacatagtg agaaggtcac tatggctggt ttgcatgagt ctgggcctat tgtgcttacg
     4021 ccgccttagt aacaaacatg ccaaaagaag gaaagctttc accagctttc aagtttaatc
     4081 aatctttgag agtcacaatt gcaagaataa atgaattttt acaacacagt tcgatgcact
     4141 gtgtttcgtc ttgatgaaaa ttggacggca aatcgagtat cttaaacaaa tctcggcata
     4201 ggctgtccat tttgtggtcg cctctttgtt ggtagtttgc agtagtttca ttagtagaag
     4261 atctgcatcc tcaatctgct aaacgtcttg agcagctatg agacgatccg gggaattggg
     4321 gcccgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     4381 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4441 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4501 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4561 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4621 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4681 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4741 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4801 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4861 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4921 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4981 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5041 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5101 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5161 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5221 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5281 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5341 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5401 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5461 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5521 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5581 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5641 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5701 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5761 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5821 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5881 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5941 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6001 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6061 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6121 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6181 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6241 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6301 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6361 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6421 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6481 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
