LOCUS       V002573                 9026 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002573
VERSION     V002573
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
   PUBMED   29497842
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 9026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Extremophiles (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux,
            Paris 75015, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    893..914
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1586..2236
                     /codon_start=1
                     /product="p24"
                     /label="p24"
                     /protein_id="AVP12426.1"
                     /translation="MESGENTPYKKFICLHCGHVFDSDRDAPKCPNCGRRKVIPRETLG
                     ESLRKHKDKLKELGVLPEPDPEPQETEVGKAEVETAGQKDPETSKPEPIQVEQKVEELK
                     PESDQEPQNSESVDSPDDVPESDKLLAKYLEELEDVPAKPKPKKLRPKKSKRSITVPAG
                     PSFLEVLIIIGAIVLIWKWLSSRSSKSDDESNVPRPSETYYQIQRNLGHHVLG"
     CDS             2771..4696
                     /codon_start=1
                     /product="Rep74"
                     /label="Rep74"
                     /protein_id="AVP12425.1"
                     /translation="MRLSNSSINSLLSSGSCDVVGSSPSGSPFLPQSTGSTQKTLSVYL
                     DISPTNEKGEPSVRGPTIRVSSHPLPAYAYYRSATGHLERQKFESQGRVKIDIYKLRKK
                     LFWIRRRLERVDLTDEERSSLEAERDRLVSLTEKLLRKIYSGSALYGDRFVIDVPVAYS
                     QLCKALGINPSDVGVSLFVRSAVMDLEFDDSSRSALKISYVSRLFAGKYHPAKGISKGF
                     KEARRVVRDLLVLQEFLDGSLLSYHKGDYVTSNSLLPMRFFVLTLPEEISYYIWSKLRE
                     GDDSALKLFKKISSQAIRDFLFYLAQKEGIPINKSYFVPGFLQNIHPTGDRDPFKPHFH
                     AHFSVVFVVYDKSSHTWYRLNPVLDEADLEKLREIWKALVVEAFSEILSGDTLTKDFNV
                     WVGDRYYSLPHDYVGVLFEIKYNARKMFVNYSSYYERNSFSDDFDRSFVSFIFDYKNRT
                     ERYGFLTNIKRYLSRLSISAVKKRLEELRELLDRIEADLLVVDSGRFPLLYQSLLDKRE
                     AVQSEISRLESVLNNPDDAFRVLYDEISRDVESMLSPRTVKENRIVSLLESLHGKRVVG
                     LSVEYIRYLTRDGEEVLDVPLHKFLEARRSVVLLSDRHKTVEFMLWWDPFWEDPPDVLE
                     LKIPNS"
     CDS             4859..6157
                     /gene="trpE"
                     /label="Anthranilate synthase component 1"
                     /note="Anthranilate synthase component 1 from Thermococcus
                     kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1).
                     Accession#: Q9YGB3"
     promoter        complement(6246..6264)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6271..6287)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6428..6856
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             7200..7991
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             8012..8869
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1021 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt
     1081 tttctgatat tctgattttc tgataatttg ccaattctgg caatttggca aattcgacaa
     1141 atttgccact gttatggcac tgttatggta ttgtgatacc aattttttgg ggtctggtac
     1201 cttactactt cttgccaact tctgccaagt tctgccaatt cttgccagtg ttgatgtgtc
     1261 cgttttgttc ctaattctcc agttttctgg ttcactgctt aaatactcct ctgccgtagc
     1321 tctgtctgac ggtgcctcac cgtctttgag ctaaaatcag gggtgagagt tgtggtcgag
     1381 ttggtgagac tggacggttc ggtgaacttt ggagacggtg ccgaggtagt gagtgtttgg
     1441 ttgaataagc ttagtgattt cgagaagctg aagtccacct tcggcggtat cttttcaacg
     1501 tcggagacgt cgaagggtgt tctttactcg ttgtttgttc cgtctttccg tgtgaggttc
     1561 ctttgtcttg tgcgaggtgg tggagatgga aagcggtgag aatacgcctt acaagaagtt
     1621 tatttgccta cactgtgggc acgtctttga cagtgatagg gacgcgccga agtgtccgaa
     1681 ctgtggccgt aggaaagtta ttccgcgtga gactcttggt gagtctctca ggaagcacaa
     1741 ggataagtta aaggaactcg gtgttcttcc tgagcctgac cctgagcctc aggagactga
     1801 ggttggaaag gctgaggttg agactgctgg ccagaaggac cctgagactt cgaagcctga
     1861 gcctatccag gttgagcaga aggttgagga gttaaaaccg gagtctgacc aggagcctca
     1921 aaactctgag tctgtggact ctccagatga tgttcctgag tcagataagc ttcttgctaa
     1981 atatctcgaa gagcttgagg acgttcctgc gaaacctaag cctaagaagt tgaggccaaa
     2041 gaaatctaag aggtccatca ctgttcccgc aggtccttcc ttcctcgaag tcctcatcat
     2101 catcggggct atagtcctca tatggaagtg gttatcttcg aggtcctcaa agtctgatga
     2161 tgagtccaac gttccgaggc cttctgagac ctattatcag attcagcgga atctcggtca
     2221 tcacgttctt gggtgatggt tatggccgaa gacaagaaga aatccaggag ctgggtagag
     2281 gttgctttct accttttcat cgctttggtt gttatctggg tgttcgtcag ggcactcaag
     2341 aagtctcaga acgtgatact atggaaccct gagcttggcc tcagactcct ggaaaaatct
     2401 ctctcaaaac ttggattctt ggggaaaagt ataaatacga tttgattctc actactattt
     2461 gcgggacgtg gtaccgatgc cgaagccgac caggagacca tcagaaatcc ttcagtggtt
     2521 ccgggaacac cctggcgaag ttacctctct caaggctcta tctctcaatc tcaatatccc
     2581 ttacaacact gtattcgttg ttgttaagaa gctggttgaa gagggtaagc ttaggaaggt
     2641 tggtcgtggt ctctacactt tggctgatga agaatcagat gctgacgata atagaagggg
     2701 aggcaccaac gcgggacgtg gtaccgatgc ctccccttcc taaagaatcc caacaggagg
     2761 ggattcgcgt atgaggttaa gtaattcctc tataaattct ttgctgtcat cggggtcttg
     2821 tgatgttgtg ggtagctcac cgagtggctc acccttccta cctcagtcta ctggttcgac
     2881 tcagaagacg cttagcgttt atcttgatat atctccaacc aatgaaaagg gtgagccatc
     2941 tgtaagaggt cctactatcc gggtttcttc tcatcctctc cctgcctatg cctactatcg
     3001 ctctgctact ggccatctcg aacgccagaa atttgaatcc cagggtcgcg tcaagattga
     3061 catctataaa cttcggaaga aactcttctg gattaggcgt agacttgagc gtgtggacct
     3121 tactgatgag gagcgctcct ctcttgaggc tgagcgagac cgtttggttt ctcttactga
     3181 gaagttgctc aggaaaatct actccggttc tgctctttat ggtgaccgct ttgttattga
     3241 tgtccccgta gcttattctc aactctgtaa agctcttggg attaatccgt ctgatgtagg
     3301 ggtctctcta tttgttcggt ctgcagttat ggatttggaa tttgatgact cctcccgttc
     3361 tgctcttaaa attagttatg tatcccgtct ttttgctggt aaataccatc ctgctaaggg
     3421 tatatctaag ggctttaagg aggctcgtag ggtagttcgc gatttacttg ttctccaaga
     3481 atttctcgat ggttctctcc tttcctacca taagggtgat tatgtcacct ctaatagtct
     3541 tctacctatg aggttctttg tcctgactct tcccgaagaa atcagctatt atatctggtc
     3601 caagcttcgt gagggggatg attcagctct aaagctcttt aagaaaatta gttctcaagc
     3661 gattagagac ttcctctttt accttgctca aaaagaggga atcccaatta acaaatccta
     3721 ttttgtcccc gggttccttc agaatattca tcccactgga gacagggacc cgtttaagcc
     3781 tcacttccac gctcacttct ctgttgtttt cgttgtttat gataagtcgt ctcatacgtg
     3841 gtaccgtttg aatcctgtcc ttgatgaggc ggatcttgag aagttgagag aaatctggaa
     3901 agctcttgta gttgaggctt tctctgaaat tctttcgggg gatactctca caaaggactt
     3961 caatgtttgg gtgggcgata ggtattactc tcttcctcac gactacgttg gagttctctt
     4021 cgaaataaag tataatgctc gcaagatgtt tgtgaactat tcgagttact atgagcgcaa
     4081 ttctttctcc gatgactttg ataggtcctt tgtgagtttc atctttgatt ataagaaccg
     4141 gactgagagg tatggtttcc tcacaaacat caaacgttat ctctctcgtc tttcaatttc
     4201 tgctgtcaag aaacgtcttg aggaacttcg ggagctttta gatagaatcg aggctgattt
     4261 gctcgttgtt gatagtggta ggttccctct cctttatcag tctctcttgg ataaacgaga
     4321 ggctgttcaa tctgaaattt cccgtcttga atctgtgttg aacaatccgg atgatgcttt
     4381 ccgtgttctc tatgatgaaa tctctcgtga tgttgagtct atgctttctc ctcgtactgt
     4441 taaagagaat cggattgttt ctttgttgga gagtcttcac ggaaaacggg ttgttggtct
     4501 ctctgttgag tatatccgtt accttacccg tgacggtgaa gaagttttgg atgttcctct
     4561 tcacaaattc cttgaggctc gtcgctcggt agttcttctt tcggatagac ataaaactgt
     4621 ggagtttatg ctgtggtggg acccgttctg ggaggaccca ccagatgtgt tagaactgaa
     4681 aattccaaat tcctgataag ggcgaattct gcagatctag agcggccgca aaaaagtcga
     4741 ctgccgcaac gcgcattttg ctcacccgaa aatttttaaa tactaagggt taatttaatc
     4801 tcgagcgttt gagtccttct gacggctctt ggagagggcc gttaaaaagg tgatgcatat
     4861 gcctctcaaa aagctgaagc ccgttgaccc tttgaagctc tacagcgccc ttagagactt
     4921 tgggatgcca ttcatgctcc gctctgccga gaaggactcc aggaaggcca gattcaccta
     4981 catatcggcc gagccggagt tcgtcgtgga ggtcggcgag gggactgaga tcgacgggga
     5041 gcgagtttcc gacgagagga accccctcag agctcttaaa gggctcatgg gggagagggt
     5101 cgagggcagg aggttcatgg gtggcttcgt tggctacgtc tcctacgatt cggtacactc
     5161 catcatcggg gggaagatcg aagagccctc ggtcttcggc tactacccct ggaccttcat
     5221 ctacgaccac tctaccggcg ctctttcctt cttttacctc agagaggctc ctttcgaccc
     5281 cgaggcctta gttgaaaggg ccaggaggga agagtcacgg cttgaagacg gcggttcgga
     5341 ggtcatatcc accgacgcgg gcatggaaga gttcgttgaa atcgtcaggg ctgggaagga
     5401 gtacatctac tcgggggacg tcttccaggt ggttctgtcg cgcgagtaca gggttagaac
     5461 ggatctcgat gccctcgaaa tctacaagcg gctcgtggag ctcaacccct ccccgtacac
     5521 cttcatcctg gagttcgaga agaccgtcgt aggggcctca cccgaaacca tgggttccgt
     5581 cgaggggaga accttcaaga taaaccccat agcaggaaca gcgccgaggg gaaggacggg
     5641 ggaggaagac cgggagctgg aaaaggccct actctccgac gagaaagagc gagctgagca
     5701 cgtcatgctc gttgaccttg ctagaaacga cgtcaggagg gtttcaaagc ccgggagcgt
     5761 taggctaacc cgcttcttcg acgtcctgaa gtacagccac gtccagcaca tagagagcga
     5821 ggtggtcggt gaactcgatg aggggaaaaa cgcgttcgac gccatggagg cggcttttcc
     5881 ggcgggaaca ctaaccggag ccccgaagat aagggcgatg gagatcatag acgagctgga
     5941 gaggagcagg agaaaggtct acgggggagc agtgggttac ttctccctca ccggggacgc
     6001 cgacatggcg atagcgataa ggatggccga gatcgagggc aggaaagcga gcgttagggc
     6061 cggggcagga atagtggcgg attcagttcc agagaaggag ttcttcgaga ccgagaacaa
     6121 aatgagagca gttctgaagg cgctgggggt gagggaatga cagagagcag cggaacggag
     6181 cgttcgcgtg gcggaaatgg ttgggactat ctgattgaag aatctatccg gagggcccaa
     6241 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     6301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6361 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6421 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6841 aaatttaacg cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca
     6901 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     6961 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     7021 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     7081 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     7141 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     7201 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7261 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7321 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7381 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7441 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7501 atctcctgtc atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7561 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7621 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7681 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     7741 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7801 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7861 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7921 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7981 acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8041 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8101 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8161 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8221 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8281 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8341 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8401 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8461 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8521 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8581 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8641 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8701 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8761 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8821 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8881 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9001 ccactgagcg tcagaccccg tagaaa
//
