LOCUS       V014323                 3810 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014323
VERSION     V014323
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2254..3312
                     /gene="MAFA"
                     /label="Transcription factor MafA"
                     /note="Transcription factor MafA from Homo sapiens.
                     Accession#: Q8NHW3"
     CDS             3313..3366
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     primer_bind     complement(3416..3432)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctg ttctagagcc accatggctg ctgaactggc tatgggtgct
     2281 gaactgccgt cttctccgct ggctatcgaa tacgttaacg acttcgacct gatgaaattc
     2341 gaagttaaaa aagaaccgcc ggaagctgaa cgtttctgcc accgtctgcc gccgggttct
     2401 ctgtcttcta ccccgctgtc taccccgtgc tcttctgttc cgtcttctcc gtctttctgc
     2461 gctccgtctc cgggtaccgg tggtggtggt ggtgctggtg gtggtggtgg ttcttctcag
     2521 gctggtggtg ctccgggtcc gccgtctggt ggtccgggtg ctgttggtgg tacctctggt
     2581 aaaccggctc tggaagacct gtactggatg tctggttacc agcaccacct gaacccggaa
     2641 gctctgaacc tgaccccgga agacgctgtt gaagctctga tcggttctgg tcaccacggt
     2701 gctcaccacg gtgctcacca cccggctgct gctgctgctt acgaagcttt ccgtggtccg
     2761 ggtttcgctg gtggtggtgg tgctgacgac atgggtgctg gtcaccacca cggtgctcac
     2821 cacgctgctc accaccacca cgctgctcac caccaccacc accaccacca ccaccacggt
     2881 ggtgctggtc acggtggtgg tgctggtcac cacgttcgtc tggaagaacg tttctctgac
     2941 gaccagctgg tttctatgtc tgttcgtgaa ctgaaccgtc agctgcgtgg tttctctaaa
     3001 gaagaagtta tccgtctgaa acagaaacgt cgtaccctga aaaaccgtgg ttacgctcag
     3061 tcttgccgtt tcaaacgtgt tcagcagcgt cacatcctgg aatctgaaaa atgccagctg
     3121 cagtctcagg ttgaacagct gaaactggaa gttggtcgtc tggctaaaga acgtgacctg
     3181 tacaaagaaa aatacgaaaa actggctggt cgtggtggtc cgggttctgc tggtggtgct
     3241 ggtttcccgc gtgaaccgtc tccgccgcag gctggtccgg gtggtgctaa aggtaccgct
     3301 gacttcttcc tggagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     3361 ggcccttccg gatgcactag tccagtcgac ggtctcgagg cagaattcca aattcactgg
     3421 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3481 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3541 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     3601 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3661 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3721 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3781 ggttttcacc gtcatcaccg aaacgcgcga
//
