LOCUS       62056_21215        4743 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1091..1807
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      2017..2064
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2159..2614
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2946..3050
                     /label=AmpR promoter
     CDS             3051..3908
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4082..4670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaggctaga gtacttaata cgactcacta taggctagca tttaggtgac actatagagg
       61 gagaccctcg accgtcgatt gtccactggt caacaataga tgacttacaa ctaatcggaa
      121 ggtgcagaga ctcgacggga gctaccctaa cgtcaagacg agggtaaaga gagagtccaa
      181 ttctcaaagc caataggcag tagcgaaagc tgcaagagaa tgaaaatccg ttgaccttaa
      241 acggtcgtgt gggttcaagt ccctccaccc ccacgccgga aacgcaatag ccgaaaaaca
      301 aaaaacaaaa aaaacaaaaa aaaaaccaaa aaaacaaaac acattaaaac agcctgtggg
      361 ttgatcccac ccacaggccc attgggcgct agcactctgg tatcacggta cctttgtgcg
      421 cctgttttat accccctccc ccaactgtaa cttagaagta acacacaccg atcaacagtc
      481 agcgtggcac accagccacg ttttgatcaa gcacttctgt taccccggac tgagtatcaa
      541 tagactgctc acgcggttga aggagaaagc gttcgttatc cggccaacta cttcgaaaaa
      601 cctagtaaca ccgtggaagt tgcagagtgt ttcgctcagc actaccccag tgtagatcag
      661 gtcgatgagt caccgcattc cccacgggcg accgtggcgg tggctgcgtt ggcggcctgc
      721 ccatggggaa acccatggga cgctctaata cagacatggt gcgaagagtc tattgagcta
      781 gttggtagtc ctccggcccc tgaatgcggc taatcctaac tgcggagcac acaccctcaa
      841 gccagagggc agtgtgtcgt aacgggcaac tctgcagcgg aaccgactac tttgggtgtc
      901 cgtgtttcat tttattccta tactggctgc ttatggtgac aattgagaga tcgttaccat
      961 atagctattg gattggccat ccggtgacta atagagctat tatatatccc tttgttgggt
     1021 ttataccact tagcttgaaa gaggttaaaa cattacaatt cattgttaag ttgaatacag
     1081 caaagccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1261 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1321 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1501 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1561 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     1621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1681 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1801 gtacaagtaa aaaaaacaaa aaacaaaacg gctattatgc gttaccggcg agacgctacg
     1861 gacttaaata attgagcctt aaagaagaaa ttctttaagt ggatgctctc aaactcaggg
     1921 aaacctaaat ctagttatag acaaggcaat cctgagccaa gccgaagtag taattagtaa
     1981 gaccagtgga caatcgacgg ataacagcat atctagctag cataacccct tggggcctct
     2041 aaacgggtct tgaggggttt tttggatccc ggcgcgccgg gatccgggct ggcgtaatag
     2101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     2161 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2221 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2281 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2341 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2401 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2461 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2521 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2581 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     2641 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     2701 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2761 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2821 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     2881 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2941 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3001 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3061 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3121 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3181 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3241 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3301 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3361 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3421 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3481 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3541 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3601 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3661 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3721 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3781 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3841 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3901 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3961 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4021 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4081 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4141 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4201 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4261 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4321 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4381 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4441 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4501 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4561 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4621 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4681 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga
     4741 tct
//
