LOCUS       V002575                10251 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002575
VERSION     V002575
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Wilcox BA, Hoang TT.
  TITLE     Engineering of tellurite-resistant genetic tools for single-copy
            chromosomal analysis of Burkholderia spp. and characterization of
            the Burkholderia thailandensis betBA operon
  JOURNAL   Appl. Environ. Microbiol. 75 (12), 4015-4027 (2009)
   PUBMED   19376905
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Wilcox BA, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Molecular Biosciences and Bioengineering,
            University of Hawaii at Manoa, 2538 McCarthy Mall, Snyder Hall 207,
            Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 10251)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2009"; volume: "75"; issue: "12";
            pages: "4015-4027"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Molecular Biosciences and Bioengineering, University
            of Hawaii at Manoa, 2538 McCarthy Mall, Snyder Hall 207, Honolulu,
            HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(600..984)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(1007..1115)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     protein_bind    1491..1512
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1527..1557
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1565..1581
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1589..1605
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1642..1670
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     mobile_element  complement(2181..2405)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             3123..5228
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     CDS             5228..6892
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             6898..8424
                     /codon_start=1
                     /gene="tnsD"
                     /product="TnsD"
                     /label="tnsD"
                     /note="Tn7 transposase subunit"
                     /protein_id="ACN91051.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            6898..8424
                     /gene="tnsD"
                     /label="tnsD"
     primer_bind     complement(8719..8735)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             9072..10172
                     /gene="asd"
                     /label="Aspartate-semialdehyde dehydrogenase"
                     /note="Aspartate-semialdehyde dehydrogenase from
                     Escherichia coli O157:H7. Accession#: P0A9R0"
ORIGIN
        1 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
       61 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      481 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
      541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagccggtt
      601 gtcagccgtt aagtgttcct gtgtcactca aaattgcttt gagaggctct aagggcttct
      661 cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc
      721 cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga
      781 tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt
      841 acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag
      901 cttagtacgt taaacttgag agcttagtac gtgaaacatg agagcttagt acgtactatc
      961 aacaggttga actgcccatg ttctttcctg cgttatcaga gcttatcggc cagcctcgca
     1021 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     1081 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     1141 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     1201 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaagt ataccttaac
     1261 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1321 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1381 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1441 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1501 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1561 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaattc
     1621 ggcttgaatt cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag
     1681 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     1741 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca
     1801 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1861 gttacgcagc agggcagtcg ccctaaaaca aagttaggca gccgttgtgc tggtgctttc
     1921 tgatagttgt tgtggggtag gcagtcagag ctcgatttgc ttgtcgccat aatagattca
     1981 caagaaggat tcgacccggg gatcctctag agtcgagatg ccgcatgtgg aagaggtgat
     2041 tgcaccgatc ttctacaccg ttccgctgca gctgctggct taccatgtcg cgctgatcaa
     2101 aggcaccgac gttgaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     2161 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaagggaa caaaatagat
     2221 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     2281 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     2341 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
     2401 tattccatgg ctaacagtac aagaagttcc ttcttcaggt cgttcccacc gtatttattc
     2461 tcataagacg ggacgagtcc atcatttgct atctgactta gagcttgctg tttttctcag
     2521 tcttgagtgg gagagcagcg tgctagatat acgcgagcag ttccccttat tacctagtga
     2581 taccaggcag attgcaatag atagtggtat taagcatcct gttattcgtg gtgtagatca
     2641 ggttatgtct actgattttt tagtggactg caaagatggt ccttttgagc agtttgctat
     2701 tcaagtcaaa cctgcagcag ccttacaaga cgagcgtacc ttagaaaaac tagaactaga
     2761 gcgtcgctat tggcagcaaa agcaaattcc ttggttcatt tttactgata aagaaataaa
     2821 tcccgtagta aaagaaaata ttgaatggct ttattcagtg aaaacagaag aagtttctgc
     2881 ggagctttta gcacaactat ccccattggc ccatatcctg caagaaaaag gagatgaaaa
     2941 cattatcaat gtctgtaagc aggttgatat tgcttatgat ttggagttag gcaaaacatt
     3001 gagtgagata cgagccttaa ccgcaaatgg ttttattaag ttcaatattt ataagtcttt
     3061 cagggcaaat aagtgtgcag atctctgtat tagccaagta gtgaatatgg aggagttgcg
     3121 ctatgtggca aattaatgag gttgtgctat ttgataatga tccgtatcgc attttggcta
     3181 tagaggatgg ccaagttgtc tggatgcaaa taagcgctga taaaggagtt ccacaagcta
     3241 gggctgagtt gttgctaatg cagtatttag atgaaggccg cttagttaga actgatgacc
     3301 cttatgtaca tcttgattta gaagagccgt ctgtagattc tgtcagcttc cagaagcgcg
     3361 aggaggatta tcgaaaaatt cttcctatta ttaatagtaa ggatcgtttc gaccctaaag
     3421 tcagaagcga actcgttgag catgtggtcc aagaacataa ggttactaag gctacagttt
     3481 ataagttgtt acgccgttac tggcagcgtg gtcaaacgcc taatgcatta attcctgact
     3541 acaaaaacag cggtgcacca ggggaaagac gttcagcgac aggaacagca aagattggcc
     3601 gagccagaga atatggtaag ggtgaaggaa ccaaggtaac gcccgagatt gaacgccttt
     3661 ttaggttgac catagaaaag cacctgttaa atcaaaaagg tacaaagacc accgttgcct
     3721 atagacgatt tgtggacttg tttgctcagt attttcctcg cattccccaa gaggattacc
     3781 caacactacg tcagtttcgt tatttttatg atcgagaata ccctaaagct cagcgcttaa
     3841 agtctagagt taaagcaggg gtatataaaa aagacgtacg acccttaagt agtacagcca
     3901 cttctcaggc gttaggccct gggagtcgtt atgagattga tgccacgatt gctgatattt
     3961 atttagtgga tcatcatgat cgccaaaaaa tcataggaag accaacgctt tacattgtga
     4021 ttgatgtgtt tagtcggatg atcacgggct tttatatcgg ctttgaaaat ccgtcttatg
     4081 tggtggcgat gcaggctttt gtaaatgctt gctctgacaa aacggccatt tgtgcccagc
     4141 atgatattga gattagtagc tcagactggc cgtgtgtagg tttgccagat gtgttgctag
     4201 cggaccgtgg cgaattaatg agtcatcagg tcgaagcctt agtttctagt tttaatgtgc
     4261 gagtggaaag tgctccacct agacgtggcg atgctaaagg catagtggaa agcactttta
     4321 gaacactaca agccgagttt aagtcctttg cacctggcat tgtagagggc agtcggatca
     4381 aaagccatgg tgaaacagac tataggttag atgcatctct gtcggtattt gagttcacac
     4441 aaattatttt gcgtacgatc ttattcagaa ataaccatct ggtgatggat aaatacgatc
     4501 gagatgctga ttttcctaca gatttaccgt ctattcctgt ccagctatgg caatggggta
     4561 tgcagcatcg tacaggtagt ttaagggctg tggagcaaga gcagttgcga gtagcgttac
     4621 tgcctcgccg aaaggtctct atttcttcat ttggcgttaa tttgtggggt ttgtattact
     4681 cggggtcaga gattctgcgt gagggttggt tgcagcggag cactgatata gctagacctc
     4741 aacatttaga agcggcttat gacccagtgc tggttgatac gatttatttg tttccgcaag
     4801 ttggcagccg tgtattttgg cgctgtaatc tgacggaacg tagtcggcag tttaaaggtc
     4861 tctcattttg ggaggtttgg gatatacaag cacaagaaaa acacaataaa gccaatgcga
     4921 agcaggatga gttaactaaa cgcagggagc ttgaggcgtt tattcagcaa accattcaga
     4981 aagcgaataa gttaacgccc agtactactg agcccaaatc aacacgcatt aagcagatta
     5041 aaactaataa aaaagaagcc gtgacctcgg agcgtaaaaa acgtgcggag catttgaagc
     5101 caagctcttc aggtgatgag gctaaagtta ttcctttcaa cgcagtggaa gcggatgatc
     5161 aagaagatta cagcctaccc acatacgtgc ctgaattatt tcaggatcca ccagaaaagg
     5221 atgagtcatg agtgctaccc ggattcaagc agtttatcgt gatacggggg tagaggctta
     5281 tcgtgataat ccttttatcg aggccttacc accattacaa gagtcagtga atagtgctgc
     5341 atcactgaaa tcctctttac agcttacttc ctctgacttg caaaagtccc gtgttatcag
     5401 agctcatacc atttgtcgta ttccagatga ctattttcag ccattaggta cgcatttgct
     5461 actaagtgag cgtatttcgg tcatgattcg aggtggctac gtaggcagaa atcctaaaac
     5521 aggagattta caaaagcatt tacaaaatgg ttatgagcgt gttcaaacgg gagagttgga
     5581 gacatttcgc tttgaggagg cacgatctac ggcacaaagc ttattgttaa ttggttgttc
     5641 tggtagtggg aagacgacct ctcttcatcg tattctagcc acgtatcctc aggtgattta
     5701 ccatcgtgaa ctcaatgtag agcaggtggt gtatttgaaa atagactgct cgcataatgg
     5761 ttcgctaaaa gaaatctgct tgaatttttt cagagcgttg gatcgagcct tgggctcgaa
     5821 ctatgagcgt cgttatggct taaaacgtca tggtatagaa accatgttgg ctttgatgtc
     5881 gcaaatagcc aatgcacatg ctttagggtt gttggttatt gatgaaattc agcatttaag
     5941 ccgctctcgt tcgggtggat ctcaagagat gctgaacttt tttgtgacga tggtgaatat
     6001 tattggcgta ccagtgatgt tgattggtac ccctaaagca cgagagattt ttgaggctga
     6061 tttgcggtct gcacgtagag gggcagggtt tggagctata ttctgggatc ctatacaaca
     6121 aacgcaacgt ggaaagccca atcaagagtg gatcgctttt acggataatc tttggcaatt
     6181 acagctttta caacgcaaag atgcgctgtt atcggatgag gtccgtgatg tgtggtatga
     6241 gctaagccaa ggagtgatgg acattgtagt aaaacttttt gtactcgctc agctccgtgc
     6301 gctagcttta ggcaatgagc gtattaccgc tggtttattg cggcaagtgt atcaagatga
     6361 gttaaagcct gtgcacccca tgctagaggc attacgctcg ggtatcccag aacgcattgc
     6421 tcgttattct gatctagtcg ttcccgagat tgataaacgg ttaatccaac ttcagctaga
     6481 tatcgcagcg atacaagaac aaacaccaga agaaaaagcc cttcaagagt tagataccga
     6541 agatcagcgt catttatatc tgatgctgaa agaggattac gattcaagcc tgttaattcc
     6601 cactattaaa aaagcgttta gccagaatcc aacgatgaca agacaaaagt tactgcctct
     6661 tgttttgcag tggttgatgg aaggcgaaac ggtagtgtca gaactagaaa agccctccaa
     6721 gagtaaaaag gtttcggcta taaaggtagt caagcccagc gactgggata gcttgcctga
     6781 tacggattta cgttatatct attcacaacg ccaacctgaa aaaaccatgc atgaacggtt
     6841 aaaagggaaa ggggtaatag tggatatggc gagcttattt aaacaagcag gttagccatg
     6901 agaaactttc ctgttccgta ctcgaatgag ctgatttata gcactattgc acgggcaggc
     6961 gtttatcaag ggattgttag tcctaagcag ctgttggatg aggtgtatgg caaccgcaag
     7021 gtggtcgcta ccttaggtct gccctcgcat ttaggtgtga tagcaagaca tctacatcaa
     7081 acaggacgtt acgctgttca gcagcttatt tatgagcata ccttattccc tttatatgct
     7141 ccgtttgtag gcaaggagcg ccgagacgaa gctattcggt taatggagta ccaagcgcaa
     7201 ggtgcggtgc atttaatgct aggagtcgct gcttctagag ttaagagcga taaccgcttt
     7261 agatactgcc ctgattgcgt tgctcttcag ctaaataggt atggggaagc cttttggcaa
     7321 cgagattggt atttgcccgc tttgccatat tgtccaaaac acggtgcttt agtcttcttt
     7381 gatagagctg tagatgatca ccgacatcaa ttttgggctt tgggtcatac tgagctgctt
     7441 tcagactacc ccaaagactc cctatctcaa ttaacagcac tagctgctta tatagcccct
     7501 ctgttagatg ctccacgagc gcaagagctt tccccaagcc ttgagcagtg gacgctgttt
     7561 tatcagcgct tagcgcagga tctagggcta accaaaagca agcacattcg tcatgacttg
     7621 gtggcggaga gagtgaggca gacttttagt gatgaggcac tagagaaact ggatttaaag
     7681 ttggcagaga acaaggacac gtgttggctg aaaagtatat tccgtaagca tagaaaagcc
     7741 tttagttatt tacagcatag tattgtgtgg caagccttat tgccaaaact aacggttata
     7801 gaagcgctac agcaggcaag tgctcttact gagcactcta taacgacaag acctgttagc
     7861 cagtctgtgc aacctaactc tgaagattta tctgttaagc ataaagactg gcagcaacta
     7921 gtgcataaat accaaggaat taaggcggca agacagtctt tagagggtgg ggtgctatac
     7981 gcttggcttt accgacatga cagggattgg ctagttcact ggaatcaaca gcatcaacaa
     8041 gagcgtctgg cacccgcccc tagagttgat tggaaccaaa gagatcgaat tgctgtacga
     8101 caactattaa gaatcataaa gcgtctagat agtagccttg atcacccaag agcgacatcg
     8161 agctggctgt taaagcaaac tcctaacgga acctctcttg caaaaaatct acagaaactg
     8221 cctttggtag cgctttgctt aaagcgttac tcagagagtg tggaagatta tcaaattaga
     8281 cggattagcc aagcttttat taagcttaaa caggaagatg ttgagcttag gcgctggcga
     8341 ttattaagaa gtgcaacgtt atctaaagag cggataactg aggaagcaca aagattcttg
     8401 gaaatggttt atggggaaga gtgagtggtt aggctagcta catttaatga caatgtgcag
     8461 gttgtacata ttggtcattt attccgtaac tcgggtcata aggagtggcg tatttttgtt
     8521 tggtttaatc caatgcaaga acggaaatgg actcgattta ctcatttgcc tttattaagt
     8581 cgagctaagg tggttaacag tacaacaaag caaataaata aggcggatcg tgtgattgag
     8641 tttgaagcat cggatcttca acgagccaaa ataatcgggg gatcctctag agtcgacctg
     8701 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8761 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8821 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaaatgat ttcattcata
     8881 actcaaattc cctgataatt gccgcggact ttctgcgtgc taacaaagca ggataagtcg
     8941 cattactgat ggcttcgcta tcattgatta atttcacttg cgactttggc tgctttttgt
     9001 atggtgaaag atgtgccaag aggagaccgg cacatttata cagcacacat ctttgcagga
     9061 aaaaaacgct tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc
     9121 tcatgcaacg catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta
     9181 cttctcagct tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg
     9241 cctttgatct ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt
     9301 ataccaacga aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg
     9361 cagcatcgtc tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg
     9421 acgtcattac cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg
     9481 taagcctgat gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt
     9541 ccgttgcaac ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa
     9601 cccagatggg ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta
     9661 ttctcgatat cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata
     9721 actttggcgt gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg
     9781 gtcagagccg cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt
     9841 ccgtaattcc ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg
     9901 cattcactat taaattgaaa aaagatgtgt ctattccgac cgtggaagaa ctgctggctg
     9961 cgcacaatcc gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa
    10021 ccccagctgc cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata
    10081 tgggaccaga gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg
    10141 agccgctgcg tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc
    10201 gatgccgccc ctgttagtgc gtaatacagg agtaagcgca gatgtttcat g
//
