LOCUS       V002577                10445 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002577
VERSION     V002577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Lee KW, Periasamy S, Mukherjee M, Xie C, Kjelleberg S, Rice SA.
  TITLE     Biofilm development and enhanced stress resistance of a model,
            mixed-species community biofilm
  JOURNAL   ISME J 8 (4), 894-907 (2014)
   PUBMED   24152718
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   Lee KWK., Periasamy S, Mukherjee M, Xie C, Kjelleberg S, Rice SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2014) Singapore Centre on Environmental Life
            Sciences Engineering, Nanyang Technological University, 60 Nanyang
            Drive, SBS-01N-27 637551, Singapore
REFERENCE   3  (bases 1 to 10445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME J";
            date: "2014"; volume: "8"; issue: "4"; pages: "894-907"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-SEP-2014) Singapore Centre on Environmental Life Sciences
            Engineering, Nanyang Technological University, 60 Nanyang Drive,
            SBS-01N-27 637551, Singapore"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(690..2216)
                     /codon_start=1
                     /gene="tnsD"
                     /product="TnsD"
                     /label="tnsD"
                     /note="Tn7 transposition site selector"
                     /protein_id="AIZ73053.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            complement(690..2216)
                     /gene="tnsD"
                     /label="tnsD"
     CDS             complement(2222..3886)
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             complement(3886..5991)
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     mobile_element  6709..6933
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     complement(7971..7987)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7995..8011
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8019..8049)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8064..8085)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            8461..8569
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      8592..8976
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(9388..10245)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10246..10350)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atcccccgat tattttggct cgttgaagat ccgatgcttc aaactcaatc
      481 acacgatccg ccttatttat ttgctttgtt gtactgttaa ccaccttagc tcgacttaat
      541 aaaggcaaat gagtaaatcg agtccatttc cgttcttgca ttggattaaa ccaaacaaaa
      601 atacgccact ccttatgacc cgagttacgg aataaatgac caatatgtac aacctgcaca
      661 ttgtcattaa atgtagctag cctaaccact cactcttccc cataaaccat ttccaagaat
      721 ctttgtgctt cctcagttat ccgctcttta gataacgttg cacttcttaa taatcgccag
      781 cgcctaagct caacatcttc ctgtttaagc ttaataaaag cttggctaat ccgtctaatt
      841 tgataatctt ccacactctc tgagtaacgc tttaagcaaa gcgctaccaa aggcagtttc
      901 tgtagatttt ttgcaagaga ggttccgtta ggagtttgct ttaacagcca gctcgatgtc
      961 gctcttgggt gatcaaggct actatctaga cgctttatga ttcttaatag ttgtcgtaca
     1021 gcaattcgat ctctttggtt ccaatcaact ctaggggcgg gtgccagacg ctcttgttga
     1081 tgctgttgat tccagtgaac tagccaatcc ctgtcatgtc ggtaaagcca agcgtatagc
     1141 accccaccct ctaaagactg tcttgccgcc ttaattcctt ggtatttatg cactagttgc
     1201 tgccagtctt tatgcttaac agataaatct tcagagttag gttgcacaga ctggctaaca
     1261 ggtcttgtcg ttatagagtg ctcagtaaga gcacttgcct gctgtagcgc ttctataacc
     1321 gttagttttg gcaataaggc ttgccacaca atactatgct gtaaataact aaaggctttt
     1381 ctatgcttac ggaatatact tttcagccaa cacgtgtcct tgttctctgc caactttaaa
     1441 tccagtttct ctagtgcctc atcactaaaa gtctgcctca ctctctccgc caccaagtca
     1501 tgacgaatgt gcttgctttt ggttagccct agatcctgcg ctaagcgctg ataaaacagc
     1561 gtccactgct caaggcttgg ggaaagctct tgcgctcgtg gagcatctaa cagaggggct
     1621 atataagcag ctagtgctgt taattgagat agggagtctt tggggtagtc tgaaagcagc
     1681 tcagtatgac ccaaagccca aaattgatgt cggtgatcat ctacagctct atcaaagaag
     1741 actaaagcac cgtgttttgg acaatatggc aaagcgggca aataccaatc tcgttgccaa
     1801 aaggcttccc catacctatt tagctgaaga gcaacgcaat cagggcagta tctaaagcgg
     1861 ttatcgctct taactctaga agcagcgact cctagcatta aatgcaccgc accttgcgct
     1921 tggtactcca ttaaccgaat agcttcgtct cggcgctcct tgcctacaaa cggagcatat
     1981 aaagggaata aggtatgctc ataaataagc tgctgaacag cgtaacgtcc tgtttgatgt
     2041 agatgtcttg ctatcacacc taaatgcgag ggcagaccta aggtagcgac caccttgcgg
     2101 ttgccataca cctcatccaa cagctgctta ggactaacaa tcccttgata aacgcctgcc
     2161 cgtgcaatag tgctataaat cagctcattc gagtacggaa caggaaagtt tctcatggct
     2221 aacctgcttg tttaaataag ctcgccatat ccactattac ccctttccct tttaaccgtt
     2281 catgcatggt tttttcaggt tggcgttgtg aatagatata acgtaaatcc gtatcaggca
     2341 agctatccca gtcgctgggc ttgactacct ttatagccga aaccttttta ctcttggagg
     2401 gcttttctag ttctgacact accgtttcgc cttccatcaa ccactgcaaa acaagaggca
     2461 gtaacttttg tcttgtcatc gttggattct ggctaaacgc ttttttaata gtgggaatta
     2521 acaggcttga atcgtaatcc tctttcagca tcagatataa atgacgctga tcttcggtat
     2581 ctaactcttg aagggctttt tcttctggtg tttgttcttg tatcgctgcg atatctagct
     2641 gaagttggat taaccgttta tcaatctcgg gaacgactag atcagaataa cgagcaatgc
     2701 gttctgggat acccgagcgt aatgcctcta gcatggggtg cacaggcttt aactcatctt
     2761 gatacacttg ccgcaataaa ccagcggtaa tacgctcatt gcctaaagct agcgcacgga
     2821 gctgagcgag tacaaaaagt tttactacaa tgtccatcac tccttggctt agctcatacc
     2881 acacatcacg gacctcatcc gataacagcg catctttgcg ttgtaaaagc tgtaattgcc
     2941 aaagattatc cgtaaaagcg atccactctt gattgggctt tccacgttgc gtttgttgta
     3001 taggatccca gaatatagct ccaaaccctg cccctctacg tgcagaccgc aaatcagcct
     3061 caaaaatctc tcgtgcttta ggggtaccaa tcaacatcac tggtacgcca ataatattca
     3121 ccatcgtcac aaaaaagttc agcatctctt gagatccacc cgaacgagag cggcttaaat
     3181 gctgaatttc atcaataacc aacaacccta aagcatgtgc attggctatt tgcgacatca
     3241 aagccaacat ggtttctata ccatgacgtt ttaagccata acgacgctca tagttcgagc
     3301 ccaaggctcg atccaacgct ctgaaaaaat tcaagcagat ttcttttagc gaaccattat
     3361 gcgagcagtc tattttcaaa tacaccacct gctctacatt gagttcacga tggtaaatca
     3421 cctgaggata cgtggctaga atacgatgaa gagaggtcgt cttcccacta ccagaacaac
     3481 caattaacaa taagctttgt gccgtagatc gtgcctcctc aaagcgaaat gtctccaact
     3541 ctcccgtttg aacacgctca taaccatttt gtaaatgctt ttgtaaatct cctgttttag
     3601 gatttctgcc tacgtagcca cctcgaatca tgaccgaaat acgctcactt agtagcaaat
     3661 gcgtacctaa tggctgaaaa tagtcatctg gaatacgaca aatggtatga gctctgataa
     3721 cacgggactt ttgcaagtca gaggaagtaa gctgtaaaga ggatttcagt gatgcagcac
     3781 tattcactga ctcttgtaat ggtggtaagg cctcgataaa aggattatca cgataagcct
     3841 ctacccccgt atcacgataa actgcttgaa tccgggtagc actcatgact catccttttc
     3901 tggtggatcc tgaaataatt caggcacgta tgtgggtagg ctgtaatctt cttgatcatc
     3961 cgcttccact gcgttgaaag gaataacttt agcctcatca cctgaagagc ttggcttcaa
     4021 atgctccgca cgttttttac gctccgaggt cacggcttct tttttattag ttttaatctg
     4081 cttaatgcgt gttgatttgg gctcagtagt actgggcgtt aacttattcg ctttctgaat
     4141 ggtttgctga ataaacgcct caagctccct gcgtttagtt aactcatcct gcttcgcatt
     4201 ggctttattg tgtttttctt gtgcttgtat atcccaaacc tcccaaaatg agagaccttt
     4261 aaactgccga ctacgttccg tcagattaca gcgccaaaat acacggctgc caacttgcgg
     4321 aaacaaataa atcgtatcaa ccagcactgg gtcataagcc gcttctaaat gttgaggtct
     4381 agctatatca gtgctccgct gcaaccaacc ctcacgcaga atctctgacc ccgagtaata
     4441 caaaccccac aaattaacgc caaatgaaga aatagagacc tttcggcgag gcagtaacgc
     4501 tactcgcaac tgctcttgct ccacagccct taaactacct gtacgatgct gcatacccca
     4561 ttgccatagc tggacaggaa tagacggtaa atctgtagga aaatcagcat ctcgatcgta
     4621 tttatccatc accagatggt tatttctgaa taagatcgta cgcaaaataa tttgtgtgaa
     4681 ctcaaatacc gacagagatg catctaacct atagtctgtt tcaccatggc ttttgatccg
     4741 actgccctct acaatgccag gtgcaaagga cttaaactcg gcttgtagtg ttctaaaagt
     4801 gctttccact atgcctttag catcgccacg tctaggtgga gcactttcca ctcgcacatt
     4861 aaaactagaa actaaggctt cgacctgatg actcattaat tcgccacggt ccgctagcaa
     4921 cacatctggc aaacctacac acggccagtc tgagctacta atctcaatat catgctgggc
     4981 acaaatggcc gttttgtcag agcaagcatt tacaaaagcc tgcatcgcca ccacataaga
     5041 cggattttca aagccgatat aaaagcccgt gatcatccga ctaaacacat caatcacaat
     5101 gtaaagcgtt ggtcttccta tgattttttg gcgatcatga tgatccacta aataaatatc
     5161 agcaatcgtg gcatcaatct cataacgact cccagggcct aacgcctgag aagtggctgt
     5221 actacttaag ggtcgtacgt cttttttata tacccctgct ttaactctag actttaagcg
     5281 ctgagcttta gggtattctc gatcataaaa ataacgaaac tgacgtagtg ttgggtaatc
     5341 ctcttgggga atgcgaggaa aatactgagc aaacaagtcc acaaatcgtc tataggcaac
     5401 ggtggtcttt gtaccttttt gatttaacag gtgcttttct atggtcaacc taaaaaggcg
     5461 ttcaatctcg ggcgttacct tggttccttc acccttacca tattctctgg ctcggccaat
     5521 ctttgctgtt cctgtcgctg aacgtctttc ccctggtgca ccgctgtttt tgtagtcagg
     5581 aattaatgca ttaggcgttt gaccacgctg ccagtaacgg cgtaacaact tataaactgt
     5641 agccttagta accttatgtt cttggaccac atgctcaacg agttcgcttc tgactttagg
     5701 gtcgaaacga tccttactat taataatagg aagaattttt cgataatcct cctcgcgctt
     5761 ctggaagctg acagaatcta cagacggctc ttctaaatca agatgtacat aagggtcatc
     5821 agttctaact aagcggcctt catctaaata ctgcattagc aacaactcag ccctagcttg
     5881 tggaactcct ttatcagcgc ttatttgcat ccagacaact tggccatcct ctatagccaa
     5941 aatgcgatac ggatcattat caaatagcac aacctcatta atttgccaca tagcgcaact
     6001 cctccatatt cactacttgg ctaatacaga gatctgcaca cttatttgcc ctgaaagact
     6061 tataaatatt gaacttaata aaaccatttg cggttaaggc tcgtatctca ctcaatgttt
     6121 tgcctaactc caaatcataa gcaatatcaa cctgcttaca gacattgata atgttttcat
     6181 ctcctttttc ttgcaggata tgggccaatg gggatagttg tgctaaaagc tccgcagaaa
     6241 cttcttctgt tttcactgaa taaagccatt caatattttc ttttactacg ggatttattt
     6301 ctttatcagt aaaaatgaac caaggaattt gcttttgctg ccaatagcga cgctctagtt
     6361 ctagtttttc taaggtacgc tcgtcttgta aggctgctgc aggtttgact tgaatagcaa
     6421 actgctcaaa aggaccatct ttgcagtcca ctaaaaaatc agtagacata acctgatcta
     6481 caccacgaat aacaggatgc ttaataccac tatctattgc aatctgcctg gtatcactag
     6541 gtaataaggg gaactgctcg cgtatatcta gcacgctgct ctcccactca agactgagaa
     6601 aaacagcaag ctctaagtca gatagcaaat gatggactcg tcccgtctta tgagaataaa
     6661 tacggtggga acgacctgaa gaaggaactt cttgtactgt tagccatgga atatagtctt
     6721 taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact tcagaaaatg
     6781 aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat aactggactg
     6841 atttcagttt acaactattc tgtctagttt aagactttat tgtcatagtt tagatctatt
     6901 ttgttccctt taagacttta ttgtccgccc acacccgctt acgcagggca tccatttatt
     6961 actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg cctttgatca
     7021 gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt gcaatcacct
     7081 cttccacatg cggcatctcg actctagagg atccccgggg ataacgcagg aaagaacatg
     7141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7621 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7921 atctaaagta tatatgagta aacttggtct gacaggaatt cgtaatcatg gtcatagctg
     7981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8281 tccacagaat caggggataa cgcaggaaag aacatgttaa ggtatacttt ccgctgcata
     8341 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
     8401 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
     8461 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     8521 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccga taagctctga
     8581 taacgcagga aagaacatgg gcagttcaac ctgttgatag tacgtactaa gctctcatgt
     8641 ttcacgtact aagctctcaa gtttaacgta ctaagctctc atgtttaacg aactaaaccc
     8701 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact
     8761 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
     8821 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac
     8881 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc
     8941 aattttgagt gacacaggaa cacttaacgg ctgacaaccg gctggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10381 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10441 tcgtc
//
