LOCUS       40924_43738        8189 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTNAg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 8189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Extremophiles (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux, 
            Paris 75015, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1586..2236
                     /codon_start=1
                     /product="p24"
                     /label=p24
                     /protein_id="AVP12421.1"
                     /translation="MESGENTPYKKFICLHCGHVFDSDRDAPKCPNCGRRKVIPRETLG
                     ESLRKHKDKLKELGVLPEPDPEPQETEVGKAEVETAGQKDPETSKPEPIQVEQKVEELK
                     PESDQEPQNSESVDSPDDVPESDKLLAKYLEELEDVPAKPKPKKLRPKKSKRSITVPAG
                     PSFLEVLIIIGAIVLIWKWLSSRSSKSDDESNVPRPSETYYQIQRNLGHHVLG"
     CDS             2771..4696
                     /codon_start=1
                     /product="Rep74"
                     /label=Rep74
                     /protein_id="AVP12420.1"
                     /translation="MRLSNSSINSLLSSGSCDVVGSSPSGSPFLPQSTGSTQKTLSVYL
                     DISPTNEKGEPSVRGPTIRVSSHPLPAYAYYRSATGHLERQKFESQGRVKIDIYKLRKK
                     LFWIRRRLERVDLTDEERSSLEAERDRLVSLTEKLLRKIYSGSALYGDRFVIDVPVAYS
                     QLCKALGINPSDVGVSLFVRSAVMDLEFDDSSRSALKISYVSRLFAGKYHPAKGISKGF
                     KEARRVVRDLLVLQEFLDGSLLSYHKGDYVTSNSLLPMRFFVLTLPEEISYYIWSKLRE
                     GDDSALKLFKKISSQAIRDFLFYLAQKEGIPINKSYFVPGFLQNIHPTGDRDPFKPHFH
                     AHFSVVFVVYDKSSHTWYRLNPVLDEADLEKLREIWKALVVEAFSEILSGDTLTKDFNV
                     WVGDRYYSLPHDYVGVLFEIKYNARKMFVNYSSYYERNSFSDDFDRSFVSFIFDYKNRT
                     ERYGFLTNIKRYLSRLSISAVKKRLEELRELLDRIEADLLVVDSGRFPLLYQSLLDKRE
                     AVQSEISRLESVLNNPDDAFRVLYDEISRDVESMLSPRTVKENRIVSLLESLHGKRVVG
                     LSVEYIRYLTRDGEEVLDVPLHKFLEARRSVVLLSDRHKTVEFMLWWDPFWEDPPDVLE
                     LKIPNS"
     CDS             4923..5396
                     /codon_start=1
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /label=pyruvoyl-dependent arginine decarboxylase
                     /protein_id="AVP12419.1"
                     /translation="MSWTTPKRAFIGAATAEGGTKLNAFDNALLKLGIGNVNLVKLSSV
                     IPAHIEWMEKVHDVPIGMLLPTVYAHIESDEPGMTISAALGVGISKNNEGGLIYEYSGY
                     CTKEEAEEMVRKMVEEGFRQRGWELGEFKVASAEITVKDKPAAAIAVVVMFPY"
     promoter        complement(5409..5427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5434..5450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5591..6019
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             6363..7154
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7175..8032
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1021 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt
     1081 tttctgatat tctgattttc tgataatttg ccaattctgg caatttggca aattcgacaa
     1141 atttgccact gttatggcac tgttatggta ttgtgatacc aattttttgg ggtctggtac
     1201 cttactactt cttgccaact tctgccaagt tctgccaatt cttgccagtg ttgatgtgtc
     1261 cgttttgttc ctaattctcc agttttctgg ttcactgctt aaatactcct ctgccgtagc
     1321 tctgtctgac ggtgcctcac cgtctttgag ctaaaatcag gggtgagagt tgtggtcgag
     1381 ttggtgagac tggacggttc ggtgaacttt ggagacggtg ccgaggtagt gagtgtttgg
     1441 ttgaataagc ttagtgattt cgagaagctg aagtccacct tcggcggtat cttttcaacg
     1501 tcggagacgt cgaagggtgt tctttactcg ttgtttgttc cgtctttccg tgtgaggttc
     1561 ctttgtcttg tgcgaggtgg tggagatgga aagcggtgag aatacgcctt acaagaagtt
     1621 tatttgccta cactgtgggc acgtctttga cagtgatagg gacgcgccga agtgtccgaa
     1681 ctgtggccgt aggaaagtta ttccgcgtga gactcttggt gagtctctca ggaagcacaa
     1741 ggataagtta aaggaactcg gtgttcttcc tgagcctgac cctgagcctc aggagactga
     1801 ggttggaaag gctgaggttg agactgctgg ccagaaggac cctgagactt cgaagcctga
     1861 gcctatccag gttgagcaga aggttgagga gttaaaaccg gagtctgacc aggagcctca
     1921 aaactctgag tctgtggact ctccagatga tgttcctgag tcagataagc ttcttgctaa
     1981 atatctcgaa gagcttgagg acgttcctgc gaaacctaag cctaagaagt tgaggccaaa
     2041 gaaatctaag aggtccatca ctgttcccgc aggtccttcc ttcctcgaag tcctcatcat
     2101 catcggggct atagtcctca tatggaagtg gttatcttcg aggtcctcaa agtctgatga
     2161 tgagtccaac gttccgaggc cttctgagac ctattatcag attcagcgga atctcggtca
     2221 tcacgttctt gggtgatggt tatggccgaa gacaagaaga aatccaggag ctgggtagag
     2281 gttgctttct accttttcat cgctttggtt gttatctggg tgttcgtcag ggcactcaag
     2341 aagtctcaga acgtgatact atggaaccct gagcttggcc tcagactcct ggaaaaatct
     2401 ctctcaaaac ttggattctt ggggaaaagt ataaatacga tttgattctc actactattt
     2461 gcgggacgtg gtaccgatgc cgaagccgac caggagacca tcagaaatcc ttcagtggtt
     2521 ccgggaacac cctggcgaag ttacctctct caaggctcta tctctcaatc tcaatatccc
     2581 ttacaacact gtattcgttg ttgttaagaa gctggttgaa gagggtaagc ttaggaaggt
     2641 tggtcgtggt ctctacactt tggctgatga agaatcagat gctgacgata atagaagggg
     2701 aggcaccaac gcgggacgtg gtaccgatgc ctccccttcc taaagaatcc caacaggagg
     2761 ggattcgcgt atgaggttaa gtaattcctc tataaattct ttgctgtcat cggggtcttg
     2821 tgatgttgtg ggtagctcac cgagtggctc acccttccta cctcagtcta ctggttcgac
     2881 tcagaagacg cttagcgttt atcttgatat atctccaacc aatgaaaagg gtgagccatc
     2941 tgtaagaggt cctactatcc gggtttcttc tcatcctctc cctgcctatg cctactatcg
     3001 ctctgctact ggccatctcg aacgccagaa atttgaatcc cagggtcgcg tcaagattga
     3061 catctataaa cttcggaaga aactcttctg gattaggcgt agacttgagc gtgtggacct
     3121 tactgatgag gagcgctcct ctcttgaggc tgagcgagac cgtttggttt ctcttactga
     3181 gaagttgctc aggaaaatct actccggttc tgctctttat ggtgaccgct ttgttattga
     3241 tgtccccgta gcttattctc aactctgtaa agctcttggg attaatccgt ctgatgtagg
     3301 ggtctctcta tttgttcggt ctgcagttat ggatttggaa tttgatgact cctcccgttc
     3361 tgctcttaaa attagttatg tatcccgtct ttttgctggt aaataccatc ctgctaaggg
     3421 tatatctaag ggctttaagg aggctcgtag ggtagttcgc gatttacttg ttctccaaga
     3481 atttctcgat ggttctctcc tttcctacca taagggtgat tatgtcacct ctaatagtct
     3541 tctacctatg aggttctttg tcctgactct tcccgaagaa atcagctatt atatctggtc
     3601 caagcttcgt gagggggatg attcagctct aaagctcttt aagaaaatta gttctcaagc
     3661 gattagagac ttcctctttt accttgctca aaaagaggga atcccaatta acaaatccta
     3721 ttttgtcccc gggttccttc agaatattca tcccactgga gacagggacc cgtttaagcc
     3781 tcacttccac gctcacttct ctgttgtttt cgttgtttat gataagtcgt ctcatacgtg
     3841 gtaccgtttg aatcctgtcc ttgatgaggc ggatcttgag aagttgagag aaatctggaa
     3901 agctcttgta gttgaggctt tctctgaaat tctttcgggg gatactctca caaaggactt
     3961 caatgtttgg gtgggcgata ggtattactc tcttcctcac gactacgttg gagttctctt
     4021 cgaaataaag tataatgctc gcaagatgtt tgtgaactat tcgagttact atgagcgcaa
     4081 ttctttctcc gatgactttg ataggtcctt tgtgagtttc atctttgatt ataagaaccg
     4141 gactgagagg tatggtttcc tcacaaacat caaacgttat ctctctcgtc tttcaatttc
     4201 tgctgtcaag aaacgtcttg aggaacttcg ggagctttta gatagaatcg aggctgattt
     4261 gctcgttgtt gatagtggta ggttccctct cctttatcag tctctcttgg ataaacgaga
     4321 ggctgttcaa tctgaaattt cccgtcttga atctgtgttg aacaatccgg atgatgcttt
     4381 ccgtgttctc tatgatgaaa tctctcgtga tgttgagtct atgctttctc ctcgtactgt
     4441 taaagagaat cggattgttt ctttgttgga gagtcttcac ggaaaacggg ttgttggtct
     4501 ctctgttgag tatatccgtt accttacccg tgacggtgaa gaagttttgg atgttcctct
     4561 tcacaaattc cttgaggctc gtcgctcggt agttcttctt tcggatagac ataaaactgt
     4621 ggagtttatg ctgtggtggg acccgttctg ggaggaccca ccagatgtgt tagaactgaa
     4681 aattccaaat tcctgataag ggcgaattct gcagattcgt tctctactca atggcatcga
     4741 gaatgatgtt ttagcgcgtt ttccccccat gggccttcta tgctacaaaa acacccccaa
     4801 atcgcctctt ttagatatag cgaaacgttt ttaagctggt aaagaagtcg cgtttgatta
     4861 aacagtctgc tcatctaata acaggaggag agtataccta aagagaaaag gaggaggttg
     4921 agatgagctg gacaacccca aagagagctt ttataggtgc cgcaaccgct gagggaggga
     4981 ctaagctgaa cgcctttgac aacgcactcc tcaagctggg cataggaaac gttaacctgg
     5041 tcaagctaag cagcgttatt cccgcacata tcgagtggat ggagaaggta cacgacgttc
     5101 cgataggaat gctcctgccg acagtttacg cccacatcga gagcgacgag ccggggatga
     5161 cgataagcgc cgcactgggc gtcgggataa gcaagaacaa cgaaggcggt ctgatctacg
     5221 agtattcggg ctactgcacc aaggaagaag ccgaggaaat ggtcaggaag atggtcgaag
     5281 aaggcttcag gcagaggggc tgggagctcg gtgagttcaa ggttgcaagc gccgagataa
     5341 ccgtcaagga caagccagcc gccgcaatag cggtcgtcgt catgttcccc tactgaggcc
     5401 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     5461 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5521 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5581 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6001 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     6061 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     6121 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     6181 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     6241 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     6301 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     6361 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6421 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6481 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6541 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6601 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6661 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6721 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6781 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6841 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6901 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6961 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7021 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7081 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7141 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8161 gttccactga gcgtcagacc ccgtagaaa
//
