LOCUS       40924_43598       11278 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTM117, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Merrell DS.
  TITLE     Expanding the Helicobacter pylori Genetic Toolbox: Modification of 
            an Endogenous Plasmid for Use as a Transcriptional Reporter and 
            Complementation Vector
  JOURNAL   Appl. Environ. Microbiol. 73 (23), 7506-7514 (2007)
  PUBMED    17921278
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Covacci A, Merrell DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2007) Microbiology and Immunology, Uniformed 
            Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
REFERENCE   3  (bases 1 to 11278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2007"; volume: "73"; issue: "23"; 
            pages: "7506-7514"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-2007) Microbiology and Immunology, Uniformed Services 
            University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..738)
                     /codon_start=1
                     /gene="r2"
                     /product="putative cAMP-induced filamentation protein"
                     /label=r2
                     /note="R2"
                     /protein_id="ABQ18301.1"
                     /translation="MKRQLHLSKLIEKLNHERKNAIKNGIYHLIQIKFSYNSNRIEGSG
                     LTYEQTAHIFDKSVLITEKNANIKLDDIFETINHFECVNYLLESYQESLSLEYFKTLHK
                     ILKKNCSDEVIGGFKKRPNFVGNSATTRPQLVESELTNLVKNYQRNLEVSLENIIDFHV
                     AFEKIHPFSDGNGRVGRLVMFKECLKNNIMPFIIENEHKAFYYRGVKEYHNTKGYLKDT
                     ILQSQDNFNEMVSYFFSK"
     gene            complement(16..738)
                     /gene="r2"
                     /label=r2
     CDS             complement(735..2339)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA replicase"
                     /label=repA
                     /note="RepA"
                     /protein_id="ABQ18302.1"
                     /translation="MEFDQLESQRSDLQKVLKELDTLPKTPQIELQKQEIQDRINKITD
                     TIIKELLSKHEIKKEELEPTLKEKPTPTKVPQTTPTPCKDLVVSTPKDKTYTTYHNNTN
                     KVNLGKLSEREANLLFAIFQMLKDQGNTLIRFEPQDLKRMLNIDISNERLSEVVIKLWD
                     SIKTADFWKISETETSIIQENYMLFSRCKIELNKPSKDLKYLEIQLNDNYQYLLNNLGM
                     GQYTSFNLLEFQRVRGKYAKTLYRLLKQYKSTGILSVEWGQFRELLDIPKDYDMRSIDN
                     FVLKIALKELHKIYPFEHLSYKKERRNSHDRRKVTHIDFYFEQLPKGETKKQKQADKQR
                     AKRDIKLIAWDIHKEIAKRNAKDTMEARFLELKTLIGYQFRHNDSGIVLQIDNTAFEKN
                     QMFLHVSYPKSKHNPQKFLVSNKTFALELLYINGYSLKKDNLLEEIDPPKIHPITNEPI
                     KEFAQYIGKTINITNFNVDQCPKGINNYLKITRIVKLNDNRICISVQDVDKPEKLLKPF
                     IAKDEKHLNNWFKKHYR"
     gene            complement(735..2339)
                     /gene="repA"
                     /label=repA
     CDS             1104..1490
                     /codon_start=1
                     /gene="f3"
                     /product="hypothetical protein"
                     /label=f3
                     /note="F3"
                     /protein_id="ABQ18303.1"
                     /translation="MLAFRVRNMQKHLILFKSGVVNLQNDPTVIVPELIADQSFQFKKP
                     SFHSVFGVSFSDFFVNIPCYELDVPFSALLVGLFLFLGFAFGQLLKVEINVRYLAPVVA
                     VSAFFFVTQVFKRVDFMEFFESDF"
     gene            1104..1490
                     /gene="f3"
                     /label=f3
     rep_origin      2666..2766
                     /label=from pHP666
                     /note="from pHP666"
     CDS             3420..4832
                     /codon_start=1
                     /gene="f4"
                     /product="putative replicase"
                     /label=f4
                     /note="F4"
                     /protein_id="ABQ18304.1"
                     /translation="MLKDYWECFNFLTYNDYEEWSNGEDFYSFIFPNCESKGEINKDFS
                     KPNAVFLYKDLKTTLNDTDKPALKRRIMLKDTWGDDYIDFVLENDLTLCSGLSYRGRHN
                     DLAHAQQMNALIFDLDGVGLKEITAFFEVIKYCEKNEPNKYFWPIPRPTFVVLSGVGLH
                     VYYVFDKPIDLFPNIKLQLKAYKYALTFNIWRYKETSKEKETQYQSINQSFRMVGSINE
                     KHGNKIIAFKTGDRVSLEYMNQYVKEDKRVDILKRFRPSKHTLAEAKLKFPEWYEKVII
                     QGQTSERKKWRIKRDVYEWWKKRCKEPKGGHRYFFMMCMAIYAYKCDVSKQELEKDMLE
                     VFEKLKDIPHTNPLTKRDVYSALESYDKGLACFKIKDIEILTALRIDRNRRNYRQQYLH
                     LKGIRALQQAINPTWRQGNGRPKNSGIKQSLIQEWRIKNPQGKKIDCHKELGLSRPTID
                     KWWNSKQLAMTT"
     gene            3420..4832
                     /gene="f4"
                     /label=f4
     CDS             complement(4806..5072)
                     /codon_start=1
                     /gene="r3"
                     /product="hypothetical protein"
                     /label=r3
                     /note="R3"
                     /protein_id="ABQ18305.1"
                     /translation="MLDIEYHNIFRKDYKKYLKNGFDSKLLDEVVLELRQQKPLAQKHK
                     DHILKGEWYPCRECHIRPDVLLVYIVKNNTLWLLRLGSHSELF"
     gene            complement(4806..5072)
                     /gene="r3"
                     /label=r3
     CDS             complement(5059..5337)
                     /codon_start=1
                     /gene="r4"
                     /product="hypothetical protein"
                     /label=r4
                     /note="R4"
                     /protein_id="ABQ18306.1"
                     /translation="MLKDVEVGVKFYKELGKLEKQLAKYQSKVLEIKKQMREIKKQYSQ
                     AKKDEKKAKNLETLQAIKDVDSGANFTKIDSLDELTNELKREIHAGY"
     gene            complement(5059..5337)
                     /gene="r4"
                     /label=r4
     CDS             5539..5883
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC mobilization protein"
                     /label=mobC
                     /protein_id="ABQ18307.1"
                     /translation="MPKLSDNKNITKRLRLNQTEWQIIQTQMQEKNLNFSQLVLNSLLT
                     QNSQTPTRSKKQKAIANKELVIELAKWGNNLNQIAKCLNTNKGAWDRLGLEQLIEISYQ
                     LEQIRSKYVG"
     gene            5539..5883
                     /gene="mobC"
                     /label=mobC
     CDS             5873..7888
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA mobilization protein"
                     /label=mobA
                     /note="MobA"
                     /protein_id="ABQ18308.1"
                     /translation="MLVKFWGINQGGGDGDGSVNYLLNERVKQGTAKVLKGDANLTKSL
                     LLSLTQKHKACVGCLSFEESNIDESLKYELMESFENALLTQEMQGRYNILWVEHTDKGR
                     LELNFVIPKIDLEKQKAFNPYYHKVDLKRIDTWKDCINLKHNFTNPKDLEKQQISNYHN
                     TKTPQGKAIFKNYKELDNYLYNAVTQGLLNSRAEIIELLQASNYEITRQGKDYISVKLP
                     NQQKAKRLKGIIYDEQFTEQRLIEQELREYSQNKDTSRERDTREISTAPNTTPRATQSL
                     IQELRDLEQQLHRHIEYKQRYYKKLHQSTSKDPREPKEQLPRVRDSQQRTQQNHANETT
                     RARETNPQEPSQEILTNAPNFHHRSSHHGGNISGGILSHHSTKQGQGIRAFTERSLSVK
                     TRNRPTKQRQSTENLQPNADQEIRGKNAGRILKQATERFNQRSRELTERERDLAQRKEQ
                     RNREFRERQKRAINQARESYSRISNAKQRLLEIRGRNLELERELRNAIPNAQHIISQIS
                     SFRESRESRIKSINANLRTERNHAQKRFTEHVRADFTTRITERSNQRARDFTSSIQRIE
                     NTIRDFRNNAQARVRTILHNLYARTRDAINTIKDIYRNAKERIRDFFQRADERLSKRME
                     QEARELHTKTTQLDRKIREHNQEIDDERSYSCGYGR"
     gene            5873..7888
                     /gene="mobA"
                     /label=mobA
     CDS             7949..8083
                     /codon_start=1
                     /gene="f2"
                     /product="hypothetical protein"
                     /label=f2
                     /note="F2"
                     /protein_id="ABQ18309.1"
                     /translation="MKEYKIFISHSWKYDNHYANLQDALKDLAEEIQPKVYEYGFLRE"
     gene            7949..8083
                     /gene="f2"
                     /label=f2
     CDS             complement(8131..8922)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9086..9110
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             9131..9844
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             10022..10210
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    10315..10455
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10641..11229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagattgaa acagactatt tagaaaagaa atagctaacc atttcattga aattgtcttg
       61 actttgcaaa atggtgtctt tcaagtagcc ttttgtattg tgatattctt tgacgcccct
      121 gtagtaaaag gctttgtgtt cgttttctat gatgaaaggc atgatattgt ttttcaaaca
      181 ttctttgaac atcactaatc gccccaccct accattgcca tcgctaaaag ggtggatctt
      241 ttcaaaggcc acatggaaat ctatgatgtt ttccaaactc acttcaaggt tgcgttggta
      301 atttttcaca agatttgtca attcgctttc aactaattgg ggtcttgttg tggcgctatt
      361 gcctacaaaa ttagggcgtt ttttgaaacc accaataact tcatcagaac aattcttttt
      421 caagattttg tgtaaagtct tgaagtattc taaactcaaa gattcttgat agctttcaag
      481 caagtaattc acgcattcaa aatgattgat agtttcaaaa atatcatcaa gtttgatatt
      541 ggcgtttttt tcagttatca gaacggattt gtcaaaaata tgagcggttt gttcataagt
      601 caaaccgctt ccctcaatgc gattagagtt gtaagaaaat ttgatttgga tcaagtggta
      661 aatgccattt ttgatagcat tctttctttc gtgattcagt ttttctatga gtttagataa
      721 atgcaactgt cttttcacct gtagtgtttc ttaaaccaat tattcaaatg tttttcatct
      781 ttagcgatga aaggtttgag cagtttctca ggcttgtcca catcttggac tgagatacag
      841 atccgattgt cattcagttt cacaattcta gtgattttca ggtaattgtt gatcccctta
      901 gggcattgat ccacattgaa gttggtgatg ttaatcgttt tgccgatgta ttgtgcaaat
      961 tccttgatag gttcgttggt gatggggtgg attttggggg gatcaatttc ttctagcaag
     1021 ttgtcttttt ttaggctgta tccattgata tatagcagtt ccaaagcgaa tgtcttgttg
     1081 gacacaagga atttttgggg gttatgcttg cttttagggt aagaaacatg caaaaacatt
     1141 tgattctttt caaaagcggt gttgtcaatc tgcaaaacga tcccactgtc attgtgcctg
     1201 aattgatagc cgatcaaagt tttcaattca agaaacctag cttccatagt gtctttggcg
     1261 tttcttttag cgatttcttt gtgaatatcc catgctatga gcttgatgtc ccttttagcg
     1321 cgttgcttgt cggcttgttt ttgtttcttg gtttcgcctt tgggcaattg ctcaaagtag
     1381 aaatcaatgt gcgttacctt gcgcctgtcg tggctgtttc ggcgttcttt tttgtaactc
     1441 aagtgttcaa aagggtagat tttatggagt tctttgagag cgatttttag gacaaaatta
     1501 tctatacttc tcatgtcata atcttttgga atgtctaaaa gttccctgaa ttgaccccat
     1561 tccacgctca aaatccctgt gcttttgtat tgcttgagca agcgatagag cgttttagcg
     1621 tatttacctc tcactctttg aaattctaag agattgaaag aagtgtattg acccatgcct
     1681 agattgttga gcaggtattg atagttatca ttgagttgga tttctaaata cttcaaatct
     1741 ttgctaggtt tgttcaattc aattttacag cggctaaaaa gcatgtaatt ttcttgaatg
     1801 attgaagttt cggtttcgct aattttccaa aaatcagcgg ttttaatgct atcccatagc
     1861 ttaataacca cttctgataa gcgctcatta gaaatatcta tgtttagcat gcgttttaaa
     1921 tcttgcggtt caaaacgaat gagggtattc ccttgatctt taagcatttg aaaaatagcg
     1981 aataaaagat tggcttccct ttcgctcaat ttccctagat tgaccttgtt agtgttgttg
     2041 tggtaggtgg tataggtttt atctttaggg gtgctaacca ctaaatcttt gcatggtgtg
     2101 ggggtggttt gtggcacttt tgttggtgtg ggtttttctt ttagagtggg ttctagttct
     2161 tcttttttga tttcatgctt tgatagtaat tctttaatga ttgtgtctgt tattttgttg
     2221 atgcggtctt gtatttcttg tttttgtagc tcaatttgtg gggtttttgg gagcgtatct
     2281 aattctttta acactttttg taagtctgat ctttgtgatt ctaattgatc aaattccact
     2341 atatactcct tgtttcggta taatagtgca agtcatgata gtatgtctat taatgagcca
     2401 acccccataa acaagggatt agcttttaat aaacctagtg aagtaaaggt gcgagaatta
     2461 caagcactag tactaacatg aataatagct tcatggtgtg atacctccta acgaggtgtc
     2521 gccctctcta tcctccaacc cactaactca accaagtcaa gctaatttct ccaaattttg
     2581 ccgcacatag gaaataccgc actcgtaaaa ctattagcac gctgttatta tagctaaaaa
     2641 ttgttaattg tgtagatatt gtaaaaagaa ctacacctag tgttggcaag aactacacct
     2701 agtgttggca agaactacac ctagtgttgg caagaactac acctagtgtt ggcaagaact
     2761 acacctttat tcattttcat caaaaaatga gaaaccatac agcttcgtta tttagggggt
     2821 tgcaaccacc aatccctcac gcgcgcgcat gcgagtaaaa catgtaaaaa caacatggtt
     2881 gttaaaacat gtaataaaaa caacgcgcat gacgcgctac atattttggg gttttagttt
     2941 ttatagattt tttagcctaa cgccaggttt gcgctatctt gtttgatttt gctaacgcaa
     3001 aaacagcttc ggctaacgcc tacgcttcca aactgcgatg cgcttttgtc catgctcgct
     3061 aacgcttcgc ttattggtgt ttgcttgtca gcaaacaatc ggtgcaaaac cgcttgtatg
     3121 cttcgctaac gctcgctagg cggttttagt atggggtaaa gcttaaaatt tgctaaattg
     3181 tgctaaaatg taaaaaaaaa tgtattgata aaaaataagc cccctttaag tgccagcttt
     3241 ggctggcggg aaatatgtag ctagagcgta gctatagtat tccgattaca tgggacttgt
     3301 aaggcaatat catgtggttt tttatttaat gatatttatt gttttaaaaa gttaaatttt
     3361 aagaaaaaat aacaataaaa gtgaattttt aaatgaaatg ataggttgga ggtcatggaa
     3421 tgttaaaaga ttattgggag tgttttaatt ttttgactta caatgattat gaagaatgga
     3481 gcaatgggga ggatttttat agttttattt ttcctaattg tgagagtaag ggtgaaataa
     3541 ataaggattt ttctaagcca aatgctgtgt ttttgtataa agacttaaag acaactctaa
     3601 atgataccga taaaccagct ttaaaacgcc gtataatgct taaagacact tggggcgatg
     3661 attatattga ttttgtgtta gaaaacgatt taacgctatg tagtggctta agttataggg
     3721 gtaggcataa tgacttagct catgctcaac aaatgaatgc tttaattttt gatttagatg
     3781 gggtagggtt aaaagaaatt accgcttttt ttgaagtgat taaatattgt gaaaaaaacg
     3841 agcctaacaa atatttttgg cctataccta gaccgacctt tgttgtttta agtggggtgg
     3901 gtttgcatgt gtattatgtc tttgataagc ctatagattt atttccaaat atcaaactcc
     3961 agcttaaagc ttataaatac gctcttacat tcaatatttg gcgttataaa gaaactagca
     4021 aagagaaaga aactcaatac caatctatca atcaatcttt tagaatggta gggagcatta
     4081 acgaaaagca tggcaataag attatagcct ttaaaacagg cgatagagtg agcttagaat
     4141 atatgaacca atatgtcaaa gaagataaga gagtggatat tttaaagcgt tttagaccga
     4201 gcaaacacac cttagcagaa gctaaactca aatttcctga gtggtatgaa aaagtcatta
     4261 ttcaagggca aacaagcgag agaaagaaat ggcgcattaa aagagatgtg tatgagtggt
     4321 ggaaaaagcg ttgcaaagag cctaagggag gacaccgata tttttttatg atgtgtatgg
     4381 ctatttatgc ttacaaatgc gatgtatcca agcaagagct agaaaaggat atgttagaag
     4441 tttttgaaaa attaaaagat ataccccaca ctaacccctt aactaagcga gatgtttata
     4501 gtgcgttaga gagctatgat aagggattgg cgtgttttaa gattaaagat attgaaatcc
     4561 ttacggcgtt aagaattgat aggaataggc gcaattacag acaacaatat ttgcatttaa
     4621 aggggataag agctttgcaa caagctatta atccgacttg gcgacaaggt aatggcagac
     4681 ctaaaaatag cggaataaaa caatctctta tacaagaatg gcgcattaaa aacccacaag
     4741 gcaaaaaaat tgattgtcat aaggaattag gtctatcgcg ccctactatt gataaatggt
     4801 ggaattcaaa acaactcgct atgactacct aacctaagca accaaagagt gttatttttg
     4861 actatataaa ccaacaaaac atcaggtcta atgtggcatt ctctacaagg ataccactcg
     4921 ccttttaata tgtggtcttt atgcttttga gctagtggtt tttgttgtct taattctaaa
     4981 actacttcat caagcagctt actatcaaag ccatttttga gatacttttt atagtctttt
     5041 ctaaatatat tgtggtattc aatatccagc atgtatctct ctttttaact catttgtaag
     5101 ctcatctaaa ctatcaattt ttgtaaaatt agccccacta tccacatctt taatagcttg
     5161 taaagtttct aaatttttag cttttttctc atctttctta gcttgtgagt attgcttttt
     5221 aatctccctc atttgttttt taatttctaa aactttactt tgatatttag ctaattgttt
     5281 ttctaatttg ccaagctcct tataaaattt aacccctact tctacatctt tcaacatctc
     5341 agctccttgt attaaacaat aattgtaaac ccattatacc taaactttaa ccaatctatg
     5401 ctaaactata attaaagggg caaggatact ttgtgtagct acaaagaacg gcgatgtgaa
     5461 aaatcgccta tccttgctag tgggacaccc cctagaaccc ccccataaat caaaattaag
     5521 ggataagtca aacaaaacat gccaaaactt tctgataata aaaacataac caaacgctta
     5581 cgactaaatc aaacagaatg gcaaattatc caaactcaaa tgcaagaaaa aaatctaaac
     5641 ttctcgcaat tagttttaaa ctccctttta actcaaaact cacaaactcc aacaagatct
     5701 aaaaaacaaa aagcgattgc taataaagaa ctagttattg aattagctaa gtggggaaac
     5761 aatttaaatc aaatcgctaa atgtctcaac actaataaag gagcatggga tagattaggt
     5821 ttagaacaac tcatagaaat cagttaccag ttagaacaaa taagatccaa gtatgttggt
     5881 taaattttgg ggcattaatc aagggggcgg cgatggtgat gggagcgtta attacttgct
     5941 taatgaaaga gtaaagcaag gtacggccaa agttttaaaa ggcgatgcta atctaactaa
     6001 aagtctttta ctctctctta ctcaaaaaca caaggcatgt gtagggtgct tatcctttga
     6061 agagtcaaat attgatgaga gtttaaaata cgaacttatg gaaagctttg aaaatgcttt
     6121 acttacacaa gaaatgcaag ggcgttataa cattttatgg gtagaacaca cagacaaggg
     6181 gcgtttggag ttaaattttg ttatacccaa aattgactta gagaaacaga aagcttttaa
     6241 cccttactat cacaaagtgg atttaaaacg cattgataca tggaaagatt gtatcaacct
     6301 aaaacacaat tttacaaacc ccaaagacct agaaaaacaa caaattagta actaccacaa
     6361 cacaaaaacc ccacaaggta aggctatctt taagaactac aaagagctag ataattacct
     6421 ttacaacgcc gtaacacaag gcttattaaa ttcaagggca gaaataatag agcttttaca
     6481 agcgagtaat tatgagatta ctagacaagg caaggattat atcagtgtca agctccctaa
     6541 ccaacaaaaa gccaaacgat tgaaaggaat aatttatgat gaacaattta ctgaacaaag
     6601 acttattgaa caagaactta gagaatatag ccagaacaaa gatacaagcc gagaaagaga
     6661 tacaagagaa atatcaacag ctcctaacac aacaccaaga gctacacaat ctctcataca
     6721 agagcttaga gacttggaac aacaactaca cagacacatt gaatacaaac aacgctacta
     6781 taaaaaatta catcaatcta cttcaaaaga cccaagagag cctaaagaac agctacctag
     6841 agttagagac agccaacaaa gaacacaaca aaaccatgca aacgaaacta caagagctag
     6901 agaaacaaat ccacaagaac ctagccaaga aatccttact aatgccccta actttcatca
     6961 taggtctagt catcatgggg gcaatattag tgggggcata ctttctcatc attctaccaa
     7021 gcaaggacaa ggaattagag ctttcacaga aagaagtctt tcagttaaaa cgagaaatag
     7081 accaactaaa caacgccaat ctacagagaa tttacaacca aatgccgacc aagaaataag
     7141 gggaaaaaat gcaggaagaa tacttaaaca agctacagag cgctttaatc agcgaagtag
     7201 agaacttaca gaacgagaaa gagatcttgc tcaaagaaaa gaacagcgaa atagagagtt
     7261 tagagaacga cagaaacgag ctattaacca agctagagaa agttacagca gaatttcaaa
     7321 cgctaagcaa cgattacttg aaattagagg cagaaacctt gaacttgaaa gagagctacg
     7381 aaacgctatc ccaaacgcac aacacattat tagccaaatt tcaagcttta gagagagcag
     7441 agaaagccga attaaatcaa ttaatgcaaa ccttagaaca gaaagaaatc acgctcaaaa
     7501 acgatttaca gagcatgtgc gagcagattt tacgacaaga attacagaac gctcaaatca
     7561 aagagcaaga gacttcacaa gctcaataca aagaattgaa aacacaatta gagattttag
     7621 aaacaacgct caagcaagag ttagaacaat cctacacaac ctttatgcaa gaacacgaga
     7681 cgcaattaac acaattaaag acatctacag aaacgcaaaa gaacgaatta gagacttctt
     7741 tcaacgagca gatgaacgac tttcaaaacg aatggaacaa gaagcaagag agcttcatac
     7801 aaaaacaaca cagctggata gaaagattag agaacacaat caagagatag acgacgagag
     7861 aagttatagt tgtgggtatg ggcggtaatt gcattattga tagctgtgtt ataatctaaa
     7921 aaacattaaa aaattataag gagtaataat gaaagaatat aagattttta ttagccattc
     7981 ttggaaatat gacaatcatt acgctaacct acaagacgct ttaaaagatc tcgccgaaga
     8041 aatacagcct aaagtctatg aatacgggtt tttgagagag tgagttagaa aatataatga
     8101 gcggccgcag acatctaaat ctaggtacta aaacaattca tccagtaaaa tataatattt
     8161 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     8221 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     8281 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     8341 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     8401 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     8461 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     8521 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     8581 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     8641 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     8701 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     8761 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     8821 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     8881 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctcc
     8941 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     9001 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     9061 aagttggcgt ataacatagt atcgaccgcg gtacccgggg atcctctaga tttaagaagg
     9121 agatatacat atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     9181 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     9241 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     9301 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     9361 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     9421 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     9481 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     9541 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     9601 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     9661 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     9721 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     9781 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     9841 caaataaatg tccagacctg cagttaactc ccgccgcatc cataccgcca gttgtttacc
     9901 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     9961 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
    10021 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
    10081 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
    10141 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
    10201 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10261 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    10321 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    10381 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gcggccgc
//
