LOCUS       40924_43593        6883 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTLR7-A-SEAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Mejia JE, Azar P, Guery J-C.
  TITLE     Functional study of the rs179008 single-nucleotide polymorphism of 
            human TLR7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Mejia JE, Azar P, Guery J-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2016) Centre de Physiopathologie de 
            Toulouse-Purpan, Inserm, CHU Purpan, BP 3028, Toulouse Cedex 3 
            31024, France
REFERENCE   3  (bases 1 to 6883)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6883)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-APR-2016) Centre de Physiopathologie de Toulouse-Purpan, Inserm,
            CHU Purpan, BP 3028, Toulouse Cedex 3 31024, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1124..2302
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     5'UTR           2308..2376
                     /label=derived from human TLR7
                     /note="derived from human TLR7"
     CDS             2410..3912
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="ILILFNIILISKLLGARWFPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    complement(3970..4091)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4256..4274)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4295..4311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4319..4335)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4343..4373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4388..4409)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4697..5285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5677..6333)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(6334..6436)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(6756..6860)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtta gttattacta gccgcgtgca aagcaccggc
      721 ggcggtagat gcggggtaag tactgaattt taattcgacc tatcccggta aagcgaaagc
      781 gacacgcttt tttttcacac atagcgggac cgaacacgtt ataagtatcg aactcctaaa
      841 gaagcagtgt agttttctgc agatgcaaaa aagatctcat gaaggaggaa cttgtgcagt
      901 ttacttaatc tgctttaaca cagctaaaag cagagaaaca gtccggaatc tgatggcaaa
      961 cacgctaaat gtaagagaag agtgtttgat gctgcagcca gctaaaattc gaggactcag
     1021 cgcagctcta ttctggttta gcttgcaaag atggataaag ttttaaacag agaggaatct
     1081 ttgcagctaa tggaccttgt aggtcttgaa aggagtggga attggctccg gtgcccgtca
     1141 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1201 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     1261 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     1321 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     1381 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     1441 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     1501 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     1561 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     1621 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     1681 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     1741 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     1801 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     1861 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     1921 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     1981 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2041 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2101 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2161 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     2221 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     2281 tcttccattt caggtgtcgt gaggaattct tggcacctct catgctctgc tctcttcaac
     2341 cagacctcta cattccattt tggaagaaga ctaaaaatgg tgtttccaat gtggacactg
     2401 aagagacaaa ttcttatcct ttttaacata atcctaattt ccaaactcct tggggctaga
     2461 tggtttcctg ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
     2521 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
     2581 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
     2641 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
     2701 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
     2761 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
     2821 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
     2881 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
     2941 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
     3001 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
     3061 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     3121 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     3181 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     3241 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     3301 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     3361 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     3421 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     3481 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     3541 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     3601 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     3661 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     3721 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     3781 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     3841 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     3901 ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg ccgcttcgag
     3961 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4021 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4081 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     4141 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     4201 cgacctcgag ggggggcccg gtacgcggcc gcaatggcgt acccagcttt tgttcccttt
     4261 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4321 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4441 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4501 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5101 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc ctttttgatc
     5401 agaaaaaaag gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc
     5461 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
     5521 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt
     5581 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg
     5641 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     5701 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     5761 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     5821 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     5881 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     5941 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     6001 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     6061 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgtaattc
     6121 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     6181 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     6241 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     6301 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     6361 cgacaactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     6421 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
     6481 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
     6541 ggtcgaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
