LOCUS       cloning_vector;        8565 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTL61T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    cloning vector; synthetic sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Linn T, St Pierre R.
  TITLE     Improved vector system for constructing transcriptional fusions that
            ensures independent translation of lacZ
  JOURNAL   J. Bacteriol. 172 (2), 1077-1084 (1990)
  PUBMED    2137119
REFERENCE   2  (bases 1 to 8565)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1990"; volume: "172"; issue: "2"; pages: 
            "1077-1084"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Draft entry and computer-readable sequence kindly submitted by 
            T.G.Linn, 15-NOV-1989.
            Authors indicate an uncharacterized mutation between bp 3274-3379, 
            which alters a EcoRI site normally present in the E.coli lacZ gene.
FEATURES             Location/Qualifiers
     source          1..8565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    116..192
                     /label=RNaseIII processing site
                     /note="RNaseIII processing site"
     misc_recomb     238..239
     CDS             256..3327
                     /label=lacZ
                     /note="beta-galactosidase"
     misc_recomb     3392..3393
     misc_recomb     3890..3891
     terminator      4091..4177
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4269..4296
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4589..4675
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4767..4794
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_recomb     4886..4887
     CDS             5378..5566
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5671..5811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5997..6585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6759..7616)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7617..7721)
                     /label=AmpR promoter
     variation       7824..7826
                     /note="a in pBR322; att in pTL61T"
     misc_recomb     7826..7827
     terminator      7846..7932
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8027..8113
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8208..8294
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(8392..8435)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcgg agctctcgag tcagcccggg cgaatcgata
       61 agcttgggct gcaggtcgac tctagaggat cctaactaac tagcgatccc gactcactat
      121 agagggacaa actcaaggtc attcgcaaga gtggccttta tgattgacct tcttccggtt
      181 aatacgaccg ggatcgagat cctaggtagg taggggcgcg gcattttaac tttctttatc
      241 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
      301 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      361 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      421 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
      481 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
      541 tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt
      601 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
      661 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
      721 aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc
      781 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc
      841 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg
      901 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat
      961 ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta
     1021 cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
     1081 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac
     1141 gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
     1201 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
     1261 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt
     1321 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
     1381 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac
     1441 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat
     1501 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg
     1561 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
     1621 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg
     1681 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
     1741 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
     1801 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc
     1861 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag
     1921 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
     1981 ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat
     2041 acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat
     2101 ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa
     2161 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg
     2221 atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca
     2281 caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc
     2341 tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc
     2401 agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc
     2461 cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag
     2521 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa
     2581 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc
     2641 gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc
     2701 cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg
     2761 ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa
     2821 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc
     2881 gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg
     2941 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt
     3001 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
     3061 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
     3121 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat
     3181 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
     3241 ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac
     3301 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
     3361 tttcgcgtaa ggaaatccat tatgtactat ttggcatggc ggccgacgcg ctgggctacg
     3421 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     3481 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     3541 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     3601 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     3661 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     3721 gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     3781 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     3841 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg ctgttttggc
     3901 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     3961 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     4021 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     4081 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     4141 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     4201 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     4261 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt
     4321 cgtcatatct acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag
     4381 gaaagcagct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     4441 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     4501 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     4561 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     4621 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     4681 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     4741 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     4801 tctacaaact cttcctgtcg tcatatctac aagccatccc cccacagata cggtaaacta
     4861 gcctcgtttt tgcatcagga aagcagtcgg gcagcgttgg gtcctggcca cgggtgcgca
     4921 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     4981 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     5041 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     5101 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     5161 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     5221 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     5281 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     5341 ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     5401 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     5461 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     5521 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5581 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5641 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     5701 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     5761 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     5821 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5881 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5941 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6001 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6061 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6121 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6181 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6241 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6301 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6361 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6421 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6481 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6541 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6601 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6961 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7021 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7081 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7141 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7201 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7261 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7321 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     7381 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7441 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7501 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7561 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7621 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7681 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7741 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7801 acgaggccct ttcgtcttca agaattaatt cccaattcca ggcatcaaat aaaacgaaag
     7861 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     7921 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     7981 cgggcaggac gcccgccata aactgccagg aattaattcc aggcatcaaa taaaacgaaa
     8041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     8101 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     8161 gcgggcagga cgcccgccat aaactgccag gaattaattc caggcatcaa ataaaacgaa
     8221 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     8281 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     8341 ggcgggcagg acgcccgcca taaactgcca ggaattaatt ccaggcatca aataaaacga
     8401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     8461 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     8521 tggcgggcag gacgcccgcc ataaactgcc aggaattggg gatcg
//
