LOCUS       40924_43573        5793 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cosmid vector pTL5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Slightom JL, Siemieniak DR, Koop BF, Hood L.
  TITLE     The complete nucleotide sequence of cosmid vector pTL5: location and
            origin of its genetic components
  JOURNAL   Gene 147 (1), 77-79 (1994)
  PUBMED    8088551
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Sutcliffe JG.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid pBR322
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 43 (PT 1), 77-90 (1979)
  PUBMED    383387
REFERENCE   3  (bases 1 to 5793)
  AUTHORS   Daniels DL, Schroeder JL, Szybalski W, Sanger F, Blattner FR.
  TITLE     Appendix I: A Molecular Map of Coliphage Lambda
  JOURNAL   (in) Hendrix,R.W. (Ed.); LAMBDA II: 519-676; Cold Spring Harbor 
            Laboratory, Cold Spring Harbor, NY (1983)
REFERENCE   4  (bases 1 to 5793)
  AUTHORS   Lund T, Grosveld FG, Flavell RA.
  TITLE     Isolation of transforming DNA by cosmid rescue
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79 (2), 520-524 (1982)
  PUBMED    6210908
REFERENCE   5  (bases 1 to 5793)
  AUTHORS   Slightom JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-1994) Jerry L. Slightom, Molecular Biology, The 
            Upjohn Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA
REFERENCE   6  (bases 1 to 5793)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1994"; volume: "147"; issue: "1"; pages: "77-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cold Spring
            Harb. Symp. Quant. Biol. 43 (PT 1), 77-90 (1979)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "(in) 
            Hendrix,R.W. (Ed.)"; volume: " LAMBDA II"; pages: " 519-676; Cold 
            Spring Harbor Laboratory, Cold Spring Harbor, NY (1983"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1982"; volume: "79"; issue: "2"; pages: 
            "520-524"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-1994) Jerry L. Slightom, Molecular Biology, The Upjohn 
            Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /label=TcR
                     /note="tetracycline efflux protein"
     terminator      1537..1631
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2114..3448
                     /note="Sequence region obtained from bacteriophage Charon
                     4A, but originally from bacteriophage phi 80. phi 80 right 
                     arm region"
     misc_feature    3437..3448
                     /label=bacteriophage Charon 4A cohesive ends
                     /note="bacteriophage Charon 4A cohesive ends"
     misc_feature    3449..3854
                     /note="DNA region from Charon 4A, originally from
                     bacteriophage lambda, left arm region"
     misc_feature    3573
                     /note="left arm of bacteriophage lambda (GenBank Accession 
                     Number J02459) differs after this position as it contains 
                     one additional G nucleotide not found in pTL5"
     CDS             4102..4290
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4395..4535
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4721..5308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     1441 tcctggccac gggtgcgcat gatctgccac attacgctcc tgtccggcaa agttacctct
     1501 gccgaagttg agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa
     1561 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1621 ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc aacgaccccc
     1681 cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt ccacctgctg
     1741 atctgcgact tatcaacgcc cacagcttcc gctgtcttct cagttccaag cattgcgatt
     1801 ttgttaagca acgcactctc gattcgtaga gcctcgttgc gtttgtttgc acgaaccata
     1861 tgtaagtatt tccttagata acaattgatt gaatgtatgc aaataaatgc atacaccata
     1921 ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg gggttattta
     1981 tgctgttgtt tttttgttac tcgggaaggg ctttacctct tccgcataaa cgcttccatc
     2041 agcgtttata gttaaaaaaa tctttcggcc tgcatgaatg gccttgttga tcgcgctttg
     2101 atatacgccg agatcttggc agtcacagca tgcgcatatc catgcttcga ccatgcgctc
     2161 acaaagtagg tgaatgcgca atgtagtacc cacatcgtca tcgctttcca ctgctctcgc
     2221 gaataaagat ggaaaatcaa tctcatggta atagtccatg aaaatccttg tattcataaa
     2281 tcctccaggt agctatatgc aaattgaaac aaaagagatg gtgatctttc taagagatga
     2341 tggaatctcc cttcagtatc ccgatggtca atgcgctgga tatgggatag atgggaatat
     2401 gctgattttt atgggacaga gttgcgaact gttcccaact aaaatcattt tgcacgatca
     2461 gcgcactacg aactttaccc acaaatagtc aggtaatgaa tcctgatata aagacaggtt
     2521 gataaatcag tcttctacgc gcatcgcacg cgcacaccgt agaaagtctt tcagttgtga
     2581 gcctgggcaa accgttaact ttcggcggct ttgctgtgcg acaggctcac gtctaaaagg
     2641 aaataaatca tgggtcataa aattatcacg ttgtccggcg cggcgacgga tgttctgtat
     2701 gcgctgtttt tccgtggcgc gttgctgtct ggtgatctgc cttctaaatc tggcacagcc
     2761 gaattgcgcg agcttggttt tgctgaaacc agacacacag caactgaata ccagaaagaa
     2821 aatcacttta cctttctgac atcagaaggg cagaaatttg ccgttgaaca cctggtcaat
     2881 acgcgttttg gtgagcagca atattgcgct tcgatgacgc ttggcgttga gattgatacc
     2941 tctgctgcac aaaaggcaat cgacgagctg gaccagcgca ttcgtgacac cgtctccttc
     3001 gaacttattc gcaatggagt gtcattcatc aaggacgccg ctatcgcaaa tggtgctatc
     3061 cacgcagcgg caatcgaaac acctcagccg gtgaccaata tctacaacat cagccttggt
     3121 atccagcgtg atgagccagc gcagaacaag gtaaccgtca gtgccgataa gttcaaagtt
     3181 aaacctggtg ttgataccaa cattgaaacg ttgatcgaaa acgcgctgaa aaacgctgct
     3241 gaatgtgcgg cgctggatgt cacaaagcaa atggcagcag acaagaaagc gatggatgaa
     3301 ctggcttcct atgtccgcac ggccatcatg atggaatgtt tccccggtgg tgttatctgg
     3361 cagcagtgcc gtcgatagta tgcaattgat aattattatc atttgcgggt cctttccggc
     3421 gatccgcctt gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg
     3481 gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct
     3541 gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc
     3601 tctgtttttg tccgtggaat gaacaatgga agtcaacaaa aagcagctgg ctgacatttt
     3661 cggtgcgagt atccgtacca ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg
     3721 cggtggcaag ggtaatgagg tgctttatga ctctgccgcc gtcataaaat ggtatgccga
     3781 aagggatgct gaaattgaga acgaaaagct gcgccgggag gttgaagaac tgcggcaggc
     3841 cagcgaggca gatcgatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     3901 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     3961 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     4021 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     4081 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
     4141 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
     4201 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
     4261 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4321 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4381 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     4441 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     4501 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     4561 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4621 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4681 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5161 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5761 agttaatagt ttgcgcaacg ttgttgccat taa
//
