LOCUS       40924_43538        7481 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTKbeta, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   MacGregor GR, Caskey CT.
  TITLE     Construction of plasmids that express E. coli beta-galactosidase in 
            mammalian cells
  JOURNAL   Nucleic Acids Res. 17 (6), 2365 (1989)
  PUBMED    2495524
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7481)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 7481)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1989"; volume: "17"; issue: "6"; pages: "2365"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        169..919
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          989..1085
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             1286..4330
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     polyA_signal    complement(4620..4754)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4865..4881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4889..4905)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4913..4943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4958..4979)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5267..5855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6029..6886)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6887..6991)
                     /label=AmpR promoter
     primer_bind     7465..7481
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgtcg acccggacgg gacgctcctg cgcctgatac agaacgaatt gcttgcaggc
       61 atctcatgag tgtgtcttcc cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc
      121 gcctgctgcg cgacggcgag ctgctcacca cccactcgac cagatccaaa tgagtcttcg
      181 gacctcgcgg gggccgctta agcggtggtt agggtttgtc tgacgcgggg ggagggggaa
      241 ggaacgaaac actctcattc ggaggcggct cggggtttgg tcttggtggc cacgggcacg
      301 cagaagagcg ccgcgatcct cttaagcacc cccccgccct ccgtggaggt gggggtttgg
      361 tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac
      421 cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg
      481 gtcctaggct caatggggac cgtatacgtg gacaggctct ggagcatccg cacgactgcg
      541 gtgatattac cggagacctt ctgcgggacg agccgggtca cgcggctgac gcggagcgtc
      601 cgttgggcga caaacaccag gacggggcac aggtacacta tcttgtcacc cggaggcgcg
      661 agggactgca ggagcttcag ggagtggcgc agctgcttca tccccgtgac ccgttgccgc
      721 gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
      781 aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
      841 gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
      901 cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatctgctcg acggtactcg
      961 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
     1021 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact
     1081 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcggcc
     1141 gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa gaagtcacca tgtcgtttac
     1201 tttgaccaac aagaacgtga ttttcgttgc cggtctggga ggcattggtc tggacaccag
     1261 caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1321 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1381 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     1441 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     1501 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     1561 caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     1621 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     1681 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     1741 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     1801 agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca
     1861 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
     1921 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
     1981 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
     2041 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
     2101 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     2161 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     2221 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     2281 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     2341 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     2401 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     2461 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     2521 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     2581 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     2641 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     2701 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     2761 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     2821 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     2881 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     2941 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     3001 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     3061 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     3121 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     3181 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     3241 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     3301 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     3361 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     3421 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     3481 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     3541 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
     3601 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     3661 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     3721 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     3781 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     3841 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     3901 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     3961 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     4021 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
     4081 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     4141 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     4201 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
     4261 cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg
     4321 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
     4381 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta
     4441 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca
     4501 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt
     4561 ccaaccgctg tttggtctgc tttctgacaa actcggcctc gactctaggc ggccgcgggg
     4621 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     4681 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4741 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     4801 tgtgggaggt tttttcggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat
     4861 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4921 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4981 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7021 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7081 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7141 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7201 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7261 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7321 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7381 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7441 cagggttttc ccagtcacga cgttgtaaaa cgacggccag t
//
